RAPD analysis of genetic diversity and population structure of Elymus sibiricus (Poaceae) native to the southeastern Qinghai-Tibet Plateau, China

Ma, Xiao; Chen, S.-Y.; Bai, Shiqie; Zhang, X.-Q.; Li, D.-X.; Zhang, C.-B.; Yan, Jiajun

doi:10.4238/2012.june.27.5

Cited by 14 publications

(12 citation statements)

References 36 publications

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“…The average band per primer was 10.8. The percentage of polymorphic bands (91.91%) was higher than the PPB values previous studies of SRAP variation (PPB = 86.48%) [ 6 ], SSR variation (PPB = 86.88%) [ 20 ], ISSR variation (PPB = 77.20%) [ 5 ], and RAPD variation (PPB = 78.65%) [ 21 ]. Previous studies showed that environment parameters are highly correlated with the magnitude and distribution of genetic diversity [ 5 , 8 ].…”

Section: Resultsmentioning

confidence: 62%

Potential of Start Codon Targeted (SCoT) Markers to Estimate Genetic Diversity and Relationships among Chinese Elymus sibiricus Accessions

et al. 2015

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Elymus sibiricus as an important forage grass and gene pool for improving cereal crops, that is widely distributed in West and North China. Information on its genetic diversity and relationships is limited but necessary for germplasm collection, conservation and future breeding. Start Codon Targeted (SCoT) markers were used for studying the genetic diversity and relationships among 53 E. sibiricus accessions from its primary distribution area in China. A total of 173 bands were generated from 16 SCoT primers, 159 bands of which were polymorphic with the percentage of polymorphic bands (PPB) of 91.91%. Based upon population structure analysis five groups were formed. The cluster analysis separated the accessions into two major clusters and three sub-clusters, similar to results of principal coordinate analysis (PCoA). The molecular variance analysis (AMOVA) showed that genetic variation was greater within geographical regions (50.99%) than between them (49.01%). Furthermore, the study also suggested that collecting and evaluating E. sibiricus germplasm for major geographic regions and special environments broadens the available genetic base and illustrates the range of variation. The results of the present study showed that SCoT markers were efficient in assessing the genetic diversity among E. sibiricus accessions.

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Section: Resultsmentioning

confidence: 62%

Potential of Start Codon Targeted (SCoT) Markers to Estimate Genetic Diversity and Relationships among Chinese Elymus sibiricus Accessions

et al. 2015

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“…For example, a number of studies have been conducted on the genetic diversity of E. caninus [25,26], E. fibrosus [27][28][29], E. alaskanus [30][31][32][33], E. trachycaulus [34,35], and E. sibiricus [36,37]. These studies have shown that each species possesses a unique pattern of genetic variability, and they are useful for understanding the genetic diversity for similar outcrossing grass species.…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity of Northern Wheatgrass (Elymus lanceolatus ssp. lanceolatus) as Revealed by Genotyping-by-Sequencing

Bhattarai

Peterson

et al. 2018

Diversity

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Recent advances in next generation sequencing technologies make genotyping-by-sequencing (GBS) more feasible for the molecular characterization of plant germplasm with complex and unsequenced genomes. This study represents the first preliminary effort using GBS to discover genome-wide genetic variants of northern wheatgrass (Elymus lanceolatus ssp. lanceolatus (Scribn. and J. G. Sm.) Gould) plants and to assess the genetic diversity present in four cultivated and six wild accessions. The effort generated the first novel set of genomic resources and 5659 single nucleotide polymorphism (SNP) markers for this tetraploid grass. The diversity analysis revealed 8.8% of SNP variation residing among the 10 accessions and 1.9% SNP variation present between cultivated and wild accessions. The Bayesian analysis identified three major clusters of the assayed samples, and the principal coordinates analysis revealed the genetic distinctness of the two accessions collected from Nevada and Wyoming. The flow cytometry analysis confirmed the tetraploid nature of some of the assayed samples and estimated the average genome size to be 9.3-9.4 Gb for this species. These findings are useful for the genetic improvement of this native grass species for forage production and rangeland reclamation. The findings are also encouraging for the broad application of genotyping-by-sequencing in the characterization of genome-wide genetic variability in non-model polyploid plants.

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“…Elymus sibiricus (Siberian wildrye) is a perennial, caespitose grass, widely distributed around the world (Ma et al, 2012 ). It usually grows on arid or semiarid mountain or valley grasslands at altitudes from 1,000 to 4,000 m in northwestern China.…”

Section: Introductionmentioning

confidence: 99%

“…It usually grows on arid or semiarid mountain or valley grasslands at altitudes from 1,000 to 4,000 m in northwestern China. It has also played an important role in native grassland restoration on the Qinghai-Tibet Plateau of China as a pioneer grass species (Ma et al, 2012 ). E. sibiricus usually serves as an important forage grass, and has been widely employed in establishing sown grasslands to develop stock raising, due to its strong adaptability, excellent tolerance to drought and cold, high crude protein content, and good palatability (Yan et al, 2007 ).…”

Section: Introductionmentioning

confidence: 99%

The Effect of Seed-Borne Fungi and Epichloë Endophyte on Seed Germination and Biomass of Elymus sibiricus

et al. 2017

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The interactive effects of asexual Epichloë (formerly known as Neotyphodium) endophytes isolated from Hordeum brevisubulatum, Elymus tangutorum and Achnatherum inebrians, and seed-borne fungi on Elymus sibiricus seeds, were determined by an in vitro study using supernatants from liquid cultures of the endophyte strains. In an 8 week greenhouse study, the effects on the seedlings growth was measured. The in vitro study was carried out with the seed-borne fungi Alternaria alternata, Bipolaris sorokiniana, Fusarium avenaceum, and a Fusarium sp. isolated from E. sibiricus. Different concentrations and combinations of the liquid cultures of endophytic fungi enhanced the interim germination, germination rate, length of coleoptile and radicle, and seedling dry weight of E. sibiricus under stress from seed-borne fungi. In the greenhouse study, different concentrations of the supernatant of the endophytes from H. brevisubulatum and E. tangutorum but not A. inebrians, signficantly (P < 0.05) enhanced E. sibiricus seed germination. There was no significant (P > 0.05) increase of the tiller numbers after 2 weeks. However, later on, there were significant (P < 0.05) increases in tiller number (4–8 weeks), seedling height (2–8 weeks) and dry weight (2–8 weeks). The application of Epichloë endophyte culture supernatants was an effective strategy to improve seed germination and growth under greenhouse conditions.

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RAPD analysis of genetic diversity and population structure of Elymus sibiricus (Poaceae) native to the southeastern Qinghai-Tibet Plateau, China

Cited by 14 publications

References 36 publications

Potential of Start Codon Targeted (SCoT) Markers to Estimate Genetic Diversity and Relationships among Chinese Elymus sibiricus Accessions

Potential of Start Codon Targeted (SCoT) Markers to Estimate Genetic Diversity and Relationships among Chinese Elymus sibiricus Accessions

Genetic Diversity of Northern Wheatgrass (Elymus lanceolatus ssp. lanceolatus) as Revealed by Genotyping-by-Sequencing

The Effect of Seed-Borne Fungi and Epichloë Endophyte on Seed Germination and Biomass of Elymus sibiricus

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