RAPD analysis of a variant of banana (Musa sp.) cv. grande naine and its propagation via shoot tip culture

Study of Genetic and Phenotypic Variability among Somaclones Induced by BAP and TDZ in Micropropagated Shoot Tips of Banana (Musa spp.) Using RAPD Markers

“…There was a significant increment in variation rate between the lowest and the highest concentrations of BAP and the results indicated that the variability index among regenerated somaclones significantly increased with increasing concentration of BAP up to 44.4 µM for all cultivars (Table 3), which is also generally in agreement with the findings reported by Venkatachalam et al (2007). Martin et al (2006) also reported the development of numerous morphological variations caused by BAP in banana cultivar 'Grande Naine' (AAA) which further supports our findings. Ray et al (2006) reported no morphological variation among the regenerated clones during in vitro micropropagation, acclimatization and transfer to the field conditions.…”

“…Ray et al (2006) showed that percentage of polymorphism by RAPD were 1.75 and 0.83 in 'Robusta' and 'Giant Governor', respectively. This significant reduction in percentage of polymorphism reported by these authors could be due to the only one concentration of BAP applied in the medium in a way that this concentration has been previously approved as optimum BAP concentration for banana micropropagation (Kalimuthu et al, 2007;Venkatachalam et al, 2007;Bairu et al, 2008;and Shirani et al, 2009) The effect of BAP on genetic variation has been previously reported for other cultivars of banana (Bairu et al, 2006;Martin et al, 2006;Ray et al, 2006;EL-Dougdoug et al, 2007;Mohamed, 2007;and Sheidai et al, 2008). Bairu et al (2006) studied the correlation between multiplication rate and somaclonal variation, their results showed that treatments with high multiplication rate caused more variation.…”

“…Somaclonal variation results in the production of new genotypes with a limited change in the original genome and molecular markers such as RAPD, RFLP, AFLP and microsatellites are ideal to identify genetic and epigenetic somaclones (Jain, 2001). Somaclonal variation has been studied to assess the potential of variability among the regenerated shoots of banana cultivars (Israeli et al, 1991;Sahijram et al, 2003;Bairu et al, 2006;Harirah and Khalid, 2006;Martin et al, 2006;Ray et al, 2006;El-Dougdoug et al, 2007;Jain et al, 2007;Mohamed, 2007;Oh et al, 2007;Venkatachalam and Sreedhar, 2007;and Sheidai et al, 2008). Although somaclonal variation is undesirable in the case of micropropagation as its primary objectives is to produce true -to -type clonal planting material, it can be useful for genetic improvement of banana (Sahijram et al, 2003;and Hwang, 2004).…”

Analysis of genetic stability of in vitro propagated potato microtubers using DNA markers

“…Extensive research has been conducted on genetic and epigenetic changes that may be attributed to the somaclonal variations during tissue culture. It was observed that presence or absence of variations during tissue culture depends upon the source of explant and the mode of regeneration (Goto et al 1998), including levels of growth substances that are used (Martin et al 2006). Plants regenerated from axillary buds or from other meristematic tissue showed the lowest tendency for genetic variation (Joshi and Dhawan 2007).…”

Genetic Fidelity Analysis in the Micropropagated Banana Derived from Immature Primordial Male Flower Bud

“…It has been reported that genetic variation in a culture line could be affected more by the genotype than by the period in culture (Smith, 1988;Vuylsteke et al, 1991;Vendrame et al, 1999). Extent of instability in Musa was found to depend on the interactions between the genotype and the tissue culture conditions by other researchers too (Vuylsteke et al, 1991;Martin et al, 2006) Table.1 Culture media used for somatic embryogenesis of Grand Naine and Rasthali As the plantlets derived from ECS of cv. Rasthali did not show any variation in the present study with respect to the ten ISSR markers, it can be inferred that either the long duration in culture environment or the recurrent subculturing had any negative influence in this AAB genotype as compared to Grand Naine which belongs to AAA genomic group.…”