RAPD analysis in the parasitoid wasp Psyttalia concolor reveals Mediterranean population structure and provides SCAR markers

Karam, N.; Guglielmino, C. R.; Bertin, Sabrina; Gomulski, Ludvik M.; Bonomi, Andrea; Baldacchino, Ferdinando; Simeone, Vito; Malacrida, Anna R.

doi:10.1016/j.biocontrol.2008.06.008

Cited by 16 publications

(9 citation statements)

References 23 publications

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“…This complex comprises several closely related taxa, most of which are difficult to distinguish morphologically, and some can hybridize (Billah et al, 2007). A strong genetic differentiation has been observed between geographically distant populations of part of these taxa (Cheyppe-Buchmann et al, 2011;Karam et al, 2008;Rugman-Jones et al, 2009), and local adaptation of some taxa to the host or to environmental conditions may occur (Rugman-Jones et al, 2009). Thanks to this diversity, it may still be possible to find appropriate biological control agents combining high levels of successful parasitism with adaptation to local environmental conditions in the target area.…”

Section: Introductionmentioning

confidence: 99%

A PCR-based method for estimating parasitism rates in the olive fly parasitoids Psyttalia concolor and P. lounsburyi (Hymenoptera: Braconidae)

et al. 2013

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Several parasitoids of the genus Psyttalia have been repeatedly introduced as biological control agents against the principal pest of olive, the fly Bactrocera oleae. However, few of the parasitoids released have become established and proved effective against B. oleae. It may however still be possible to find effective biological control agents adapted to local environmental conditions among the highly diverse Psyttalia species and populations infesting B. oleae worldwide. For this purpose, we have developed a rapid, sensitive molecular method based on the polymerase chain reaction (PCR) for estimating and comparing the parasitism success of Psyttalia parasitoids through the detection of eggs and larvae within the host. This method was tested and shown to be appropriate for two Psyttalia species (Psyttalia concolor and Psyttalia lounsburyi). The possible detection of DNA was also demonstrated for several populations of these species and for other Psyttalia species, namely Psyttalia humilis and Psyttalia ponerophaga. For P. concolor and P. lounsburyi, a strong correlation was observed between the parasitism rates estimated by PCR, host larva dissection and counts of emerging parasitoids. No significant difference was found between the rates of parasitism estimated by host larva dissection and PCR, whereas the rates of parasitism estimated by PCR were significantly higher than those estimated from emergence, suggesting occurrence of mortality during the parasitoid development. This PCR method is thus highly reliable and provides an objective criterion for estimating the efficacy of biological control agent candidates from diverse taxa and populations of Psyttalia.⇑ Corresponding author.

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Section: Introductionmentioning

confidence: 99%

A PCR-based method for estimating parasitism rates in the olive fly parasitoids Psyttalia concolor and P. lounsburyi (Hymenoptera: Braconidae)

et al. 2013

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“…The origin of P. concolor used in most of the early European field releases was Tunisia (Rugman-Jones et al 2009) and, for this reason, material collected throughout much of Europe today comprises relatively similar genetic groupings of P. concolor (Karam et al 2008). Psyttalia humilis (Silvestri) is a close relative of P. concolor, first described with material reared from Medfly collected in South Africa (Rugman-Jones et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Comparative evaluation of two olive fruit fly parasitoids under varying abiotic conditions

et al. 2010

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Psyttalia lounsburyi (Silvestri) and P. humilis (Silvestri) (Hymenoptera: Braconidae) were evaluated in California for their potential to control the invasive olive fruit fly, Bactrocera oleae (Rossi) (Diptera: Tephritidae). Psyttalia lounsburyi is a specialist on B. oleae while P. humilis also attacks other tephritid species. Field cage trials, conducted from 2006 to 2009, were used to compare P. lounsburyi and two populations of P. humilis (Kenya and Namibia) in California's interior valley and coastal regions. Both parasitoid species reproduced on B. oleae in all trials. Under similar abiotic conditions, offspring production per female was higher in P. humilis than in P. lounsburyi, suggesting that host specificity by P. lounsburyi does not confer a higher efficiency on B. oleae in cultivated olives. Two abiotic factors were shown to impact parasitoid efficiency. First, adult parasitoid survival was poor during periods of high summer temperatures, common to the olive production areas in California's interior valleys. Second, parasitism levels were lower on B. oleae larvae feeding in larger Ascolano cv. fruit than in smaller Manzanillo cv. fruit. Results are discussed relative to biological control of B. oleae in commercial olives and the usefulness of natural enemies specialized to attack fruit flies in wild olives compared with the larger cultivated olive fruit.

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“…Greater differences may exist among geographically distinct natural populations within the P. concolor complex, which are widely distributed in Africa (Wharton et al 2000, Mkize et al 2008. Recent molecular analyses showed clear separation of the northern Africa and sub-Saharan populations (Karam et al 2008, Rugman-Jones et al 2009). Most of the early European programs used P. concolor from northern Africa, speciÞcally Tunisia, Libya, and Morocco (Clausen 1978, Wharton 1989a.…”

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Response of <I>Psyttalia humilis</I> (Hymenoptera: Braconidae) to Olive Fruit Fly (Diptera: Tephritidae) and Conditions in California Olive Orchards

Yokoyama

Rendón²,

Wang

et al. 2011

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RAPD analysis in the parasitoid wasp Psyttalia concolor reveals Mediterranean population structure and provides SCAR markers

Cited by 16 publications

References 23 publications

A PCR-based method for estimating parasitism rates in the olive fly parasitoids Psyttalia concolor and P. lounsburyi (Hymenoptera: Braconidae)

A PCR-based method for estimating parasitism rates in the olive fly parasitoids Psyttalia concolor and P. lounsburyi (Hymenoptera: Braconidae)

Comparative evaluation of two olive fruit fly parasitoids under varying abiotic conditions

Response of <I>Psyttalia humilis</I> (Hymenoptera: Braconidae) to Olive Fruit Fly (Diptera: Tephritidae) and Conditions in California Olive Orchards

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