Rapana thomasiana hemocyanin modified with ionic liquids with enhanced anti breast cancer activity

Guncheva, Maya; Paunova, Krasimira; Ossowicz, Paula; Rozwadowski, Zbigniew; Januś, Ewa; Idakieva, Krassimira; Todinova, Svetla; Raynova, Yuliana; Uzunova, Veselina; Apostolova, Sonia; Tzoneva, Rumiana; Yancheva, Denitsa

doi:10.1016/j.ijbiomac.2015.10.031

Cited by 13 publications

(10 citation statements)

References 34 publications

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“…Renewed interests in Hc structural complexities and the assemblages of associated sugars and lipids aim to exploit the vast therapeutic potential of these megamolecules (Table 1). Especially from molluscs, Hcs are tested for application as bio-adjuvants (viral and bacterial antigens/haptens), immune-stimulants for treatment of cancers such as melanoma, and carrier molecules for vaccines (Table 1) [37–40]. …”

Section: The Oxygen-transport Proteinsmentioning

confidence: 99%

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Immunological properties of oxygen-transport proteins: hemoglobin, hemocyanin and hemerythrin

Coates

Decker

2016

Cell. Mol. Life Sci.

123

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It is now well documented that peptides with enhanced or alternative functionality (termed cryptides) can be liberated from larger, and sometimes inactive, proteins. A primary example of this phenomenon is the oxygen-transport protein hemoglobin. Aside from respiration, hemoglobin and hemoglobin-derived peptides have been associated with immune modulation, hematopoiesis, signal transduction and microbicidal activities in metazoans. Likewise, the functional equivalents to hemoglobin in invertebrates, namely hemocyanin and hemerythrin, act as potent immune effectors under certain physiological conditions. The purpose of this review is to evaluate the true extent of oxygen-transport protein dynamics in innate immunity, and to impress upon the reader the multi-functionality of these ancient proteins on the basis of their structures. In this context, erythrocyte–pathogen antibiosis and the immune competences of various erythroid cells are compared across diverse taxa.Electronic supplementary materialThe online version of this article (doi:10.1007/s00018-016-2326-7) contains supplementary material, which is available to authorized users.

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Section: The Oxygen-transport Proteinsmentioning

confidence: 99%

“…Especially from molluscs, Hcs are tested for application as bio-adjuvants (viral and bacterial antigens/haptens), immune-stimulants for treatment of cancers such as melanoma, and carrier molecules for vaccines (Table 1) [37–40]. …”

Section: The Oxygen-transport Proteinsmentioning

confidence: 99%

Immunological properties of oxygen-transport proteins: hemoglobin, hemocyanin and hemerythrin

Coates

Decker

2016

Cell. Mol. Life Sci.

123

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“…efficient therapeutic agents and strategies have important clinical values. 5,6 Traditional herbal medicines have long been used to treat various diseases. 7 Some critical components of medical herbs have been identified and show attractive anti-cancer effects in many cancers, including breast cancer.…”

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confidence: 99%

Baicalein inhibits breast cancer growth via activating a novel isoform of the long noncoding RNA PAX8‐AS1‐N

Cao

Tang

et al. 2018

J of Cellular Biochemistry

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Baicalein, a natural flavonoid, has fascinating anti-cancer properties in breast cancer. Long noncoding RNAs (lncRNAs), a class of transcripts with no protein-coding potential, also exhibit critical roles in breast cancer. However, the molecular mechanisms mediating the anti-cancer properties of baicalein and whether lncRNAs are involved in the anti-cancer effects are still unclear. In this study, we identified a novel isoform of lncRNA PAX8-AS1 (PAX8-AS1-N), which is activated by baicalein in a dose- and time-dependent manner. Functional assays showed that PAX8-AS1-N reduced cell viability, inhibited cell-cycle progression, and induced apoptosis of breast cancer cells in vitro. Depletion of PAX8-AS1-N promoted breast xenograft tumor growth in vivo. Furthermore, depletion of PAX8-AS1-N attenuated the suppressive roles of baicalein on cell viability, the apoptosis induced by baicalein, and also the suppressive roles of baicalein on tumor growth in vivo. Mechanistically, PAX8-AS1-N bound to miR-17-5p, and up-regulated miR-17-5p targets, such as PTEN, CDKN1A, and ZBTB4. In addition, PAX8-AS1-N was down-regulated in breast cancer and reduced expression of PAX8-AS1-N indicated poor survival of breast cancer patients. In conclusion, our results demonstrated that PAX8-AS1-N activation mediated the anti-cancer effects of baicalein via regulating miR-17-5p, and suggested that baicalein and enhancing PAX8-AS1-N would be potential therapeutic strategies against breast cancer.

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“…We have found that ILs with cholinium or 1-ethyl-3-methylimidazolium cation and anions polar or hydrophobic amino acids induce changes in the secondary structure of Hcs from Rapana thomasiana and Helix pomatia. However, the IL-Hcs complexes are characterized by enhancing antiproliferative effects against hormone-dependent (MCF-7) and hormone-independent (MDA-MB-231) cell lines, and, in some cases, an enhanced selectivity has been observed [29][30][31]. The effect of ILs on the stability and structure of Hcs has not been studied in detail.…”

Section: Introductionmentioning

confidence: 99%

Structural, Thermal, and Storage Stability of Rapana Thomasiana Hemocyanin in the Presence of Cholinium-Amino Acid-Based Ionic Liquids

et al. 2021

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Novel biocompatible compounds that stabilize proteins in solution are in demand for biomedical and/or biotechnological applications. Here, we evaluated the effect of six ionic liquids, containing mono- or dicholinium [Chol]1or2 cation and anions of charged amino acids such as lysine [Lys], arginine [Arg], aspartic acid [Asp], or glutamic acid [Glu], on the structure, thermal, and storage stability of the Rapana thomasiana hemocyanin (RtH). RtH is a protein with huge biomedicinal potential due to its therapeutic, drug carrier, and adjuvant properties. Overall, the ionic liquids (ILs) induce changes in the secondary structure of RtH. However, the structure near the Cu-active site seems unaltered and the oxygen-binding capacity of the protein is preserved. The ILs showed weak antibacterial activity when tested against three Gram-negative and three Gram-positive bacterial strains. On the contrary, [Chol][Arg] and [Chol][Lys] exhibited high anti-biofilm activity against E. coli 25213 and S. aureus 29213 strains. In addition, the two ILs were able to protect RtH from chemical and microbiological degradation. Maintained or enhanced thermal stability of RtH was observed in the presence of all ILs tested, except for RtH-[Chol]2[Glu].

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Rapana thomasiana hemocyanin modified with ionic liquids with enhanced anti breast cancer activity

Cited by 13 publications

References 34 publications

Immunological properties of oxygen-transport proteins: hemoglobin, hemocyanin and hemerythrin

Immunological properties of oxygen-transport proteins: hemoglobin, hemocyanin and hemerythrin

Baicalein inhibits breast cancer growth via activating a novel isoform of the long noncoding RNA PAX8‐AS1‐N

Structural, Thermal, and Storage Stability of Rapana Thomasiana Hemocyanin in the Presence of Cholinium-Amino Acid-Based Ionic Liquids

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