Raoultella sp. SM1, a novel iron-reducing and uranium-precipitating strain

Skłodowska, Aleksandra; Mielnicki, Sebastian; Drewniak, Łukasz

doi:10.1016/j.chemosphere.2017.12.123

Cited by 10 publications

(6 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A Raoutella species was recently recorded as an iron‐reducing and uranium‐precipitating strain by Sklodowska et al . (). Pramanik et al .…”

Section: Discussionmentioning

confidence: 97%

“…Raoultella ornithinolytica and planticola strains are recorded as molybdate-reducing bacteria for the first time in this study. A Raoutella species was recently recorded as an iron-reducing and uranium-precipitating strain by Sklodowska et al (2018). Pramanik et al (2016) stated that Raoutella is useful in bioremediation of Cr(VI) in Crcontaminated agricultural soil and it might also enhance plant growth promotion.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Production of molybdenum blue by two novel molybdate‐reducing bacteria belonging to the genusRaoultellaisolated from Egypt and Iraq

2019

View full text Add to dashboard Cite

Aim The aim of this study was to isolate potential molybdate‐reducing bacteria to be used for bioremediation. Methods and results Two molybdate‐reducing bacteria (Mo1 and MoI) were isolated from polluted soil samples from Ismailia Canal, Egypt and Sallah Elddin Governorate, Iraq respectively. The isolates exhibited dark blue colonies when grown on solid medium containing sodium molybdate which indicated the reduction of molybdate to molybdenum (Mo) blue. The absorbance values at 865 nm were 0·743 ± 0·007 and 0·453 ± 0·005 for Mo1 and MoI respectively. The Mo blue produced showed characteristic absorption spectrum peak at 865 nm and a shoulder at 700 nm. The isolates were identified by 16S rRNA genes sequencing and were submitted to GenBank as Raoultella ornithinolytica strain Mo1 and Raoultella planticola strain MoI. The optimum conditions were glucose as electron donor, initial pH of 6 and incubation temperature of 30°C. Scanning electron micrographs were taken for both isolates in the presence and absence of molybdate source. Conclusion To the best of our knowledge, this is the first recordation of molybdate reduction by Raoultella sp. isolated from Egypt and Iraq. Significance and Impact of the Study The isolated bacteria belonging to the Raoultella could be used in in situ bioremediation.

show abstract

“…A Raoutella species was recently recorded as an iron‐reducing and uranium‐precipitating strain by Sklodowska et al . (). Pramanik et al .…”

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Production of molybdenum blue by two novel molybdate‐reducing bacteria belonging to the genusRaoultellaisolated from Egypt and Iraq

2019

View full text Add to dashboard Cite

show abstract

“…strains X13 [34] and SM1 [35] Delftia sp. BAs29 [36] and Delftia acidovorans [37] have all shown resistance along with metal reduction or biosorption capacity on cadmium, uranium, arsenate, and gold, respectively. In this study, however, these bacteria isolates Stenotrophomonas (A1 LY), Raoultella (Isolates 4 and 5), Achromobacter (Isolate 6), Delftia (A3SWS), and Cupriavidus (C3 SD) demonstrated very low resistance to the tested metals especially under combined acidic and metal-containing (i.e., copper, iron, and zinc) experimental conditions.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of an acid and metal tolerant Enterobacter cloacae NZS strain from former mining lake in Selangor, Malaysia

Mazalan

Oyeleye

Rahman

et al. 2020

Beni-Suef Univ J Basic Appl Sci

View full text Add to dashboard Cite

Background: Metal polluted environments have been found to harbor acid and metal tolerant bacterial communities. Metal oxidizing bacteria in particular are industrially important microorganisms that can be utilized for potential applications in biomining and bioremediation. However, some well-characterized strains are not readily culturable as they are obligate and fastidious chemolithotrophs requiring special techniques for their cultivation. Hence, this study was aimed at isolating, identifying, and characterizing indigenous metal tolerant heterotroph(s) from abandoned mines that can potentially be used for biomining or bioremediation processes in the future. Results: Seventeen bacteria from former mining lakes were isolated and identified using 16S rRNA. Minimal inhibition concentration (MIC) and growth study of isolated bacteria carried out in Luria-Bertani media containing three different metals ions, zinc (II), copper (II), and iron (II), showed that a particular isolate termed Enterobacter cloacae NZS was found to exhibit better growth and tolerance for copper (up to 90 mM), zinc (up to 200 mM), and iron (up to 170 mM). Growth of the strain was notably well in the presence of iron (II). Compared to all the isolates, only E. cloacae NZS was able to be enumerated at pH lower than 5 while other strains were culturable only at pH 7. Its capability in iron (II) oxidation was preliminary assessed based on the pH, cell count, glucose consumption, and amount of iron oxidized throughout incubation in 9K media. E. cloacae NZS strain was found to be capable of oxidizing iron (II) supplied in 9K media to iron (III). Conclusion: As preliminary investigation showed that E. cloacae NZS was able to oxidize iron (II) in 9K media at pH2, further optimization on the strain, medium, and culture conditions in future may be able to provide a better insight on this strain to be possibly used as an iron oxidizer for various applications.

show abstract

“…The roles of Raoultella spp. in degrading some pollutants, such as pesticides [ 19 ] or uranium [ 20 ], have been examined by many researchers. Our goal in this study was to identify local metal-resistant microorganisms and characterize the mechanisms of metal uptake by transmission electron microscope and EDS.…”

Section: Introductionmentioning

confidence: 99%

Raoultella ornithinolytica as a Potential Candidate for Bioremediation of Heavy Metal from Contaminated Environments

Salih,

Rasheed,

Muhammed

2023

J. Microbiol. Biotechnol.

View full text Add to dashboard Cite

Disposal of waste containing heavy metals into the environment is a major threat to human health and can result in toxic or chronic poisoning in aquatic life. In the current study, metal-resistant Raoultella ornithinolytica was isolated from metal-contaminated samples collected from the Tanjaro River, located southwest of Sulaymaniyah, Iraq. R. ornithinolytica was identified by partial amplification of 16S rRNA. The uptake potency of heavy metals was assessed using inductively coupled plasma-optical emission spectroscopy (ICP-OES) and indicated that R. ornithinolytica removed 67, 89, 63.4, 55.6, 56.5, 65, and 61.9% of Cd, Pb, Cr, Ni, Zn, Co, and Fe, respectively. These removal rates were influenced by temperature, pH, and contact time; at 35°C and pH 5 with a change in the incubation time, the reduction rate improved from 89 to 95% for Pb, from 36.4 to 45% for Cu, and from 55.6 to 64% for Ni. Gene analysis indicated that R. ornithinolytica contained pbrT , chrB , nccA , iroN , and czcA genes, but the pcoD gene was absent. Energy-dispersive X-ray spectroscopy (EDS) images showed evidence of metal ion binding on the cell wall surface with different rates of binding. Transmission electron microscopy (TEM) detected different mechanisms for metal particle localization; cell surface adsorption was the main mechanism for Pb, Zn, and Co uptake, while Cd, Ni, and Fe were accumulated inside the cell. The current study describes, for the first time, the isolation of R. ornithinolytica from metal-contaminated water, which can be used as an eco-friendly biological expedient for the remediation and detoxification of metals from contaminated environments.

show abstract

Raoultella sp. SM1, a novel iron-reducing and uranium-precipitating strain

Cited by 10 publications

References 15 publications

Production of molybdenum blue by two novel molybdate‐reducing bacteria belonging to the genusRaoultellaisolated from Egypt and Iraq

Production of molybdenum blue by two novel molybdate‐reducing bacteria belonging to the genusRaoultellaisolated from Egypt and Iraq

Isolation and characterization of an acid and metal tolerant Enterobacter cloacae NZS strain from former mining lake in Selangor, Malaysia

Raoultella ornithinolytica as a Potential Candidate for Bioremediation of Heavy Metal from Contaminated Environments

Contact Info

Product

Resources

About