Raoultella ornithinolytica and Klebsiella oxytoca pyogenic liver abscess presenting as chronic cough

Surani, Asif; Slama, Eliza; Thomas, Samantha; Ross, Robert W.; Cunningham, Steven C.

doi:10.1016/j.idcr.2020.e00736

Cited by 14 publications

(10 citation statements)

References 12 publications

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“… A single bacterium was used to represent bacteria that were indistinguishable by the MBT 7854 MSP Library * E. anophelis is misidentified as E. meningoseptica by MALDI-TOF MS [ 115 ] ** R. ornithinolytica is misidentified as Klebsiella pneumonia or K. oxytoca [ 116 , 117 ] …”

Section: Methodsmentioning

confidence: 99%

Microbiota identified from preserved Anopheles

Silva

Matsena-Zingoni

Koekemoer

et al. 2021

Malar J

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Background Mosquito species from the Anopheles gambiae complex and the Anopheles funestus group are dominant African malaria vectors. Mosquito microbiota play vital roles in physiology and vector competence. Recent research has focused on investigating the mosquito microbiota, especially in wild populations. Wild mosquitoes are preserved and transported to a laboratory for analyses. Thus far, microbial characterization post-preservation has been investigated in only Aedes vexans and Culex pipiens. Investigating the efficacy of cost-effective preservatives has also been limited to AllProtect reagent, ethanol and nucleic acid preservation buffer. This study characterized the microbiota of African Anopheles vectors: Anopheles arabiensis (member of the An. gambiae complex) and An. funestus (member of the An. funestus group), preserved on silica desiccant and RNAlater® solution. Methods Microbial composition and diversity were characterized using culture-dependent (midgut dissections, culturomics, MALDI-TOF MS) and culture-independent techniques (abdominal dissections, DNA extraction, next-generation sequencing) from laboratory (colonized) and field-collected mosquitoes. Colonized mosquitoes were either fresh (non-preserved) or preserved for 4 and 12 weeks on silica or in RNAlater®. Microbiota were also characterized from field-collected An. arabiensis preserved on silica for 8, 12 and 16 weeks. Results Elizabethkingia anophelis and Serratia oryzae were common between both vector species, while Enterobacter cloacae and Staphylococcus epidermidis were specific to females and males, respectively. Microbial diversity was not influenced by sex, condition (fresh or preserved), preservative, or preservation time-period; however, the type of bacterial identification technique affected all microbial diversity indices. Conclusions This study broadly characterized the microbiota of An. arabiensis and An. funestus. Silica- and RNAlater®-preservation were appropriate when paired with culture-dependent and culture-independent techniques, respectively. These results broaden the selection of cost-effective methods available for handling vector samples for downstream microbial analyses.

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Section: Methodsmentioning

confidence: 99%

Microbiota identified from preserved Anopheles

Silva

Matsena-Zingoni

Koekemoer

et al. 2021

Malar J

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“…K. oxytoca is the second most prevalent Klebsiella in the clinic after K. pneumoniae ( Neog et al, 2021 ). Opportunistic K. oxytoca in hospitals mainly infects infants, the elderly, and patients with low immune function, and is the main cause of colitis, endocarditis, sepsis, and urinary and respiratory tract infections ( Beaugerie et al, 2003 ; Ménard et al, 2010 ; Egger et al, 2017 ; Tsubouchi et al, 2019 ; Soto-Hernández et al, 2020 ; Surani et al, 2020 ). The prevalence rate of K. oxytoca ranges from 2.3 to 24%, accounting for 13–24% of the total nosocomial bacteremia ( Watanakunakorn and Jura, 1991 ; Hansen et al, 1998 ; Manohar et al, 2017 ; Ahmad et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Characteristics of a Bacteriophage, vB_Kox_ZX8, Isolated From Clinical Klebsiella oxytoca and Its Therapeutic Effect on Mice Bacteremia

Zhang

Yan

et al. 2021

Front. Microbiol.

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Klebsiella oxytoca is an important nosocomial and community-acquired opportunistic pathogenic Klebsiella and has become the second most prevalent strain in the clinic after K. pneumoniae. However, there have been few reports of bacteriophages used for treating K. oxytoca. In this study, a novel bacteriophage, vB_Kox_ZX8, which specifically infects K. oxytoca AD3, was isolated for the first time from human fecal samples. The biological characteristics of vB_Kox_ZX8 showed an incubation period of 10 min, a burst size of 74 PFU/cell, and a stable pH range of 3–11. Genomic bioinformatics studies of vB_Kox_ZX8 showed that it belongs to the genus Przondovirus, subfamily Studiervirinae, family Autographiviridae. The genome of vB_Kox_ZX8 is 39,398 bp in length and contains 46 putative open reading frames encoding functional proteins, such as DNA degradation, packaging, structural, lysin-holin, and hypothetical proteins. We further investigated the efficacy of vB_Kox_ZX8 phage in the treatment of mice with bacteremia caused by K. oxytoca infection. The results showed that vB_Kox_ZX8 (5 × 109 PFU/mouse) injected intraperitoneally alone was metabolized rapidly in BALB/c mice, and no significant side effects were observed in the control and treatment groups. Importantly, intraperitoneal injection with a single dose of phage vB_Kox_ZX8 (5 × 107 PFU/mouse) for 1 h post-infection saved 100% of BALB/c mice from bacteremia induced by intraperitoneal challenge with a minimum lethal dose of K. oxytoca AD3. However, all negative control mice injected with PBS alone died. Owing to its good safety, narrow host infectivity, high lysis efficiency in vitro, and good in vivo therapeutic effect, phage vB_Kox_ZX8 has the potential to be an excellent antibacterial agent for clinical K. oxytoca-caused infections.

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“…This would be an extremely rare presentation, and there is only one prior report of HCC presenting with a cough [ 9 ]. Other cases of chronic cough thought to be secondary to diaphragm irritation from liver diseases have been described in the literature, including a case of a giant cavernous hemangioma, where the cough resolved after hepatic lobectomy [ 10 ], and one of a pyogenic liver abscess, where the cough resolved after percutaneous drainage [ 11 ].…”

Section: Discussionmentioning

confidence: 99%

A Chronic Cough and Something More: An Unusual Presentation of Hepatocellular Carcinoma

Cuartas-Mesa¹,

Noboa²,

Choroomi³

et al. 2023

Cureus

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Liver cancer is one of the main contributors to cancer-related death and is the fifth most frequent cancer worldwide. Hepatocellular carcinoma (HCC), the most common of liver cancers, is most frequently diagnosed incidentally during routine imaging in high-risk patients with cirrhosis. However, patients with advanced disease may present with upper abdominal pain, early satiety, weight loss, and a palpable upper abdominal mass. We describe a case of HCC in a 69-year-old male presenting exclusively with an intractable cough, which improved after transarterial chemoembolization-raising awareness of the importance of having a systematic and physiopathology-based approach to chronic cough to have an adequate diagnosis and treatment.

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Raoultella ornithinolytica and Klebsiella oxytoca pyogenic liver abscess presenting as chronic cough

Cited by 14 publications

References 12 publications

Microbiota identified from preserved Anopheles

Microbiota identified from preserved Anopheles

Characteristics of a Bacteriophage, vB_Kox_ZX8, Isolated From Clinical Klebsiella oxytoca and Its Therapeutic Effect on Mice Bacteremia

A Chronic Cough and Something More: An Unusual Presentation of Hepatocellular Carcinoma

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