Flow development downstream of a spacer grid is dependent on the upstream conditions and the imposed interface topology, especially at inlet and outlet boundaries. In STAR-CCM+, all interfaces fall into two groups, direct and indirect. A direct interface directly joins together two boundaries composing the interface either permanently or temporarily, for the case of rigid body motion. An explicit connection is created between cells on each side of the interface, so that mass and energy or either of them will occur across the interface. Three options of interface topology namely, in-place, periodic and repeating are available to be imposed at the inlet-outlet boundaries for a flow problem. In the present work, computational fluid dynamic simulation using STAR-CCM+ was performed for the flow of water at a bundle's Reynolds number of Re1 = 3.4 × 10 4 through a 5 × 5 rod bundle geometry supported by spacer grid with and without split mixing vanes for which the rod-to-rod pitch to diameter ratio was 1.33 and the rod to wall pitch to diameter ratio was 0.74. The two-layer k-epsilon turbulence model with an all y+ automatic wall treatment function in STAR-CCM+ was adopted for an isothermal single phase (water) flow through the geometry with and without imposed cyclic periodic interface boundary condition of fully developed flow type at inlet and outlet boundaries. The objectives were to primarily investigate the extent of predictability of the experimental data by the Computational Fluid Dynamic (CFD) simulation as a measure of reliability on the CFD code employed, and