Randomly amplified polymorphic DNA analysis of four different populations of the Indian major carp, Labeo rohita (Hamilton)

Islam, M. S.; Alam, Samsul

doi:10.1111/j.1439-0426.2004.00588.x

Cited by 39 publications

(46 citation statements)

References 15 publications

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“…The second clade was further separated into two subgroups, Ado-Ekiti and Esaodo populations in one sub-group, and Akure, Ilesa and Owena populations in the other subgroup. The genetic distance and identity of C. gariepinus from six populations ( (Sultana et al, 2010), C. catla (Rahman et al, 2009) and L. rohita (Islam and Alam, 2004) using RAPD marker analysis. High quality bands with good reproducibility generated Garg et al (2010) who reported 172 to 1677bp in C. batrachus.…”

Section: Resultsmentioning

confidence: 99%

GENETIC VARIABILITY IN CULTURED AND WILD POPULATIONS OF Clarias gariepinus (Osteichthys: Clariidae) USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKER

Popoola

Fasakin

Awopetu

2014

CJF

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Three wild populations of Clarias gariepinus from Esaodo (River Osun), Owena (River Owena), and Agbabu (River Oluwa), and three farmed populations, viz: Akure, Ilesa and Ado-Ekiti, in Southwest Nigeria, were analysed for their genetic differences using Random amplified polymorphic DNA (RAPD) analysis. Live specimens comprising 40 individuals (680 ± 3.28 g) from each location were collected and kept in six concrete tanks (2x1x1m). Altogether 435 reproducible bands were obtained from six populations for the nine RAPD primers used. The Analysis of Molecular Variance (AMOVA) indicated that the sampled populations are significantly different from each other, and that 99% of the total variation resided within the population. The percentage of genetic identity (GI) of RAPD-PCR profile among six populations ranged from 74.6% to 83.5%, while Genetic distance of the six populations based on RAPD-PCR profile ranged from 0.180 to 0.293. Estimates of genetic variation in wild and cultured populations of C. gariepinus were made, and total and mean number of segregating fragments were 71 (89.9%), 34.5 and 59 (74.7%), 35.4, respectively. Total gene diversity within wild and cultured populations (Ht) was 0.3419 and 0.3010, respectively. The study established that there is genetic variability in both wild and cultured C. gariepinus. RAPD showed that samples within the wild and cultured populations under study were closer to each other than between the two habitats. With reference to total gene diversity values and total number of segregating fragments, the wild population was considerably more diverse than the cultured population.

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Section: Resultsmentioning

confidence: 99%

GENETIC VARIABILITY IN CULTURED AND WILD POPULATIONS OF Clarias gariepinus (Osteichthys: Clariidae) USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKER

Popoola

Fasakin

Awopetu

2014

CJF

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“…Approximately 30-40 mg of tissue (pleopod/uropod) from each collected sample was cut into small pieces with scissors, homogenised by a micro-tissue-grinder in a 1.5-ml microfuge tube and the total DNA was extracted by Proteinase-K digestion, phenol:chloroform:isoamyl alcohol extraction and the ethanol precipitation method, as described by Islam and Alam (2004). The DNA quality was checked by electrophoresis in 1% agarose gel and quantified using a spectrophotometer (Spectronic 1 Genesis TM , Spectronic Instruments Inc., USA).…”

Section: Extraction Of Dna From Shrimp Tissuementioning

confidence: 99%

Prevalence of white spot syndrome virus infection detected by one-step and nested PCR in selected tiger shrimp (Penaeus monodon) hatcheries

2007

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White spot syndrome (WSS) is considered as a great threat to commercial farming of the tiger shrimp (Penaeus monodon). The causal agent of WSS is a DNA virus called white spot syndrome virus (WSSV). The prevalence of this dreadful virus infection has been studied in five randomly selected hatcheries located in the Cox's Bazar district of Bangladesh. Both one-step and nested polymerase chain reaction (PCR) involving two pairs of primers, namely, 146F1/146R1 and 146F2/146R2, amplifying the 1447 bp and 941 bp fragments, respectively, were conducted to detect the WSSV. Out of 60 randomly collected shrimps, 12 (20%) were found to be positive by one-step PCR, while 18 (30%) were found to be positive by nested PCR. The nested PCR was found to be much more sensitive than the one-step PCR. The shrimp specimens showing clinical signs of WSS were positive for WSSV by both one-step and nested PCR. Some of the apparently healthy samples were also found to be positive for WSSV by nested PCR. Among the two primer-pairs, the inner pair amplifying the 941 bp fragment was more sensitive than the outer primer pair amplifying the 1447 bp fragment when used in onestep PCR.

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“…Barman et al (2003) reported 45% of polymorphic loci in four Indian major carp population using RAPD primers. Islam and Alam (2004) observed 46.5% of polymorphic loci in four different Labeo rohita population. In the present study, relatively high level of genetic polymorphism was observed due to the small sampling size.…”

Section: Polymerase Chain Reactionmentioning

confidence: 89%

Genetic Diversity Analysis of Lates calcarifer (Bloch 1790) in Captive and Wild Populations Using RAPD Markers

et al. 2012

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Lates calcarifer (Bloch 1790) is one of the major economically important cultivable fish species in India. In this study, three populations of L. calcarifer was selected to assess the genetic diversity. Of which, two wild (Mudaslodai, Muthupettai) and one captive (Mutukadu) population. The genetic diversity of three populations of this species was studied using Random Amplified Polymorphic DNA (RAPD) markers. Ten random primers were used for the assessment of their genetic diversity and construction of the dendrogram. A total of 589 scorable bands were obtained, 93.12% of them were polymorphic. The Nei's gene diversity (H) of two wild populations were more (0.0504 ± 0.0670 and 0.0519 ± 0.0953) than the captive population (0.0489 ± 0.0850). The clustering pattern obtained by UPGMA method emphasized the wild populations were clustered in one clade and captive population was deviated into another clade. This study proved that RAPD analysis has the ability to discriminate L. calcarifer populations. Further molecular studies, comprising a higher number of molecular tools are still required to precisely evaluate the genetic structure of all seabass populations along the Indian coast.

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Randomly amplified polymorphic DNA analysis of four different populations of the Indian major carp, Labeo rohita (Hamilton)

Cited by 39 publications

References 15 publications

GENETIC VARIABILITY IN CULTURED AND WILD POPULATIONS OF Clarias gariepinus (Osteichthys: Clariidae) USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKER

GENETIC VARIABILITY IN CULTURED AND WILD POPULATIONS OF Clarias gariepinus (Osteichthys: Clariidae) USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKER

Prevalence of white spot syndrome virus infection detected by one-step and nested PCR in selected tiger shrimp (Penaeus monodon) hatcheries

Genetic Diversity Analysis of Lates calcarifer (Bloch 1790) in Captive and Wild Populations Using RAPD Markers

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