Randomized controlled trial on the impact of early-life intervention with bifidobacteria on the healthy infant fecal microbiota and metabolome

Bazanella, Monika; Maier, Tanja; Clavel, Thomas; Lagkouvardos, Ilias; Lucio, Marianna; Maldonado-Gómez, María X.; Autran, Chloe; Walter, Jens; Bode, Lars; Schmitt‐Kopplin, Philippe; Haller, Dirk

doi:10.3945/ajcn.117.157529

Cited by 132 publications

(162 citation statements)

References 54 publications

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“…Clear differences in the trajectory of microbial community development have been shown between breastfed and formula-fed infants [30,31]. The study by Bazanella et al was designed to evaluate the effects of a probiotic-supplemented formula intervention on the composition and function of the infant gut over the first year of life [32]. In this randomized, double-blind, placebo-controlled, clinical trial new-born infants were fed a standard whey-based formula supplemented with Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium longum and Bifidobacterium longum subspeces infantus or a control formula with no supplements.…”

Section: Infant and Pediatric Healthmentioning

confidence: 99%

The Application of Metabolomics to Probiotic and Prebiotic Interventions in Human Clinical Studies

O’Connell

2020

Metabolites

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There is an ever-increasing appreciation for our gut microbiota that plays a crucial role in the maintenance of health, as well as the development of disease. Probiotics are live bacteria that are consumed to increase the population of beneficial bacteria and prebiotics are dietary substrates intended to promote the propagation of beneficial bacteria. In order to optimize the use of probiotics and prebiotics, a more complete biochemical understanding of the impact that these treatments have on the community and functioning of the gut microbiota is required. Nucleic acid sequencing methods can provide highly detailed information on the composition of the microbial communities but provide less information on the actual function. As bacteria impart much of their influence on the host through the production of metabolites, there is much to be learned by the application of metabolomics. The focus of this review is on the use of metabolomics in the study of probiotic and prebiotic treatments in the context of human clinical trials. Assessment of the current state of this research will help guide the design of future studies to further elucidate the biochemical mechanism by which probiotics and prebiotics function and pave the way toward more personalized applications.

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Section: Infant and Pediatric Healthmentioning

confidence: 99%

The Application of Metabolomics to Probiotic and Prebiotic Interventions in Human Clinical Studies

O’Connell

2020

Metabolites

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“…Other studies have looked at adding probiotics to infant formula [24,39,40] . For example, supplementation of nonhuman primates with a formula containing B. animalis subsp.…”

Section: Human Milk and Infant Healthmentioning

confidence: 99%

“…bifidum , B. breve , B. longum , and B . infantis resulted in changes in both the structure and function (assessed through the fecal metabolome) of colonic bacteria during the period of supplementation but had no detectable long-term effects [39] . Another study where infant formula was supplemented with a probiotic/prebiotic combination of B. animalis subsp.…”

Section: Human Milk and Infant Healthmentioning

confidence: 99%

Metabolomics in Human Milk Research

Slupsky¹

2019

Nestlé Nutrition Institute Workshop Series

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The link between food and health is complex, particularly for the developing neonate, as the period after birth is the time when long-term programming is occurring notably in the neurologic, immune, and metabolic regulatory systems. Breastfeeding is known to have short-and long-term benefits, and yet the intricate relationship of this unique food with the neonate is not fully understood. Application of multi-omic approaches incorporating new bioinformatic tools will allow for better characterization of phenotypes over the traditional approaches that were limited to crude assessment of growth parameters and observation of clinical disease. Metabolomics has the capability of allowing for a relatively noninvasive assessment of phenotypes via the assessment of small molecules in biofluids such as serum or urine that provides an opportunity to assess metabolism systemically in the developing neonate. Metabolomics can also be used to assess the metabolic activities of gut microbes through measurement of microbial by-products in the stool. Understanding the composition of human milk, how its components work synergistically together, and how they change over time will provide insight into how immunity and metabolism is established in early life, and how it can potentially prevent the development of chronic diseases in later life.

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“…Bifidobacteria, most frequently isolated from the faeces of breast-fed infants, are involved in the maintenance of intestinal microbial balance and health. Bifidobacteria exert their biological activities through the production of vitamins and antimicrobial substances; further, they regulate the immune system and have anti-obesity and antiinflammatory activities 5,6 . Bifidobacteria are gram-positive, anaerobic, and saccharolytic, and have been reported to inhabit the intestinal tract of mammals and insects, the human oral cavity and sewage 7 .…”

Section: Introductionmentioning

confidence: 99%

Deciphering the metabolic capabilities of Bifidobacteria using genome-scale metabolic models

Devika

Raman

2019

Preprint

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Bifidobacteria, the initial colonisers of breastfed infant guts, are considered as the key commensals that promote a healthy gastrointestinal tract. However, little is known about the key metabolic differences between different strains of these bifidobacteria, and consequently, their suitability for their varied commercial applications. In this context, the present study applies a constraint-based modelling approach to differentiate between 36 important bifidobacterial strains, enhancing their genomescale metabolic models obtained from the AGORA (Assembly of Gut Organisms through Reconstruction and Analysis) resource. By studying various growth and metabolic capabilities in these enhanced genome-scale models across 30 different nutrient environments, we classified the bifidobacteria into three specific groups. We also studied the ability of the different strains to produce short chain fatty acids, finding that acetate production is niche-and strain-specific, unlike lactate. Further, we captured the role of critical enzymes from the bifid shunt pathway, which was found to be essential for a subset of bifidobacterial strains. Our findings underline the significance of analysing metabolic capabilities as a powerful approach to explore distinct properties of the gut microbiome. Overall, our study presents several insights into the nutritional lifestyles of bifidobacteria and could potentially be leveraged to design species/strainspecific probiotics or prebiotics.

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Randomized controlled trial on the impact of early-life intervention with bifidobacteria on the healthy infant fecal microbiota and metabolome

Cited by 132 publications

References 54 publications

The Application of Metabolomics to Probiotic and Prebiotic Interventions in Human Clinical Studies

The Application of Metabolomics to Probiotic and Prebiotic Interventions in Human Clinical Studies

Metabolomics in Human Milk Research

Deciphering the metabolic capabilities of Bifidobacteria using genome-scale metabolic models

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