2017
DOI: 10.1111/mpp.12590
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Random mutagenesis screen shows that Phytophthora capsici CRN83_152‐mediated cell death is not required for its virulence function(s)

Abstract: SummaryWith the increasing availability of plant pathogen genomes, secreted proteins that aid infection (effectors) have emerged as key factors that help to govern plant–microbe interactions. The conserved CRN (CRinkling and Necrosis) effector family was first described in oomycetes by their capacity to induce host cell death. Despite recent advances towards the elucidation of CRN virulence functions, the relevance of CRN‐induced cell death remains unclear. In planta over‐expression of PcCRN83_152, a CRN effec… Show more

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Cited by 9 publications
(14 citation statements)
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References 50 publications
(131 reference statements)
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“…While P. capsici effector CRN83_152 enhanced N. benthamiana susceptibility to P. capsici, in line with previous reports (Stam et al, 2013b) (Mafurah et al, 2015;Amaro et al, 2018) .Aphid effector Mp64 did not affect host susceptibility to P. capsici (Fig. S7), pointing to distinct virulence activities of these effectors.…”
Section: Nuclear Aphid Effector Mp64 Enhances Arabidopsis Susceptibil...supporting
confidence: 90%
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“…While P. capsici effector CRN83_152 enhanced N. benthamiana susceptibility to P. capsici, in line with previous reports (Stam et al, 2013b) (Mafurah et al, 2015;Amaro et al, 2018) .Aphid effector Mp64 did not affect host susceptibility to P. capsici (Fig. S7), pointing to distinct virulence activities of these effectors.…”
Section: Nuclear Aphid Effector Mp64 Enhances Arabidopsis Susceptibil...supporting
confidence: 90%
“…S2), we were unable to obtain yeast co-transformants expressing both CRN83_152 and full-length SIZ1 in repeated transformation experiments that included transformation controls. We also included a mutant of CRN83_152, called CRN83_152_6D10 (Amaro et al, 2018), which does not trigger CRN-cell death activation but retains virulence activity, in co-transformation experiments with similar results. To test for in planta effector-SIZ1 interactions, we co-expresssed GFP-Mp64 and GFP-CRN83_152_6D10 with either AtSIZ1-myc or NbSIZ1-myc in N. benthamiana (Fig.…”
Section: Resultsmentioning
confidence: 99%
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“…This result is consistent with the observation that PvRxLR16, when co-expressed with PvRxLR1, did not trigger cell death or immune responses in tobacco plants. Actually, effector-induced cell death can be suppressed even by variants of the effector itself; for example, four “no cell death” variants of PcCRN83_152 could suppress PcCRN83_152-mediated cell death (Amaro et al, 2018). Therefore, we speculate that there are interactions among effectors in the same pathogen isolate, suggesting that an isolate of P. viticola may not induce cell death or immune responses in grapevine if PvAvh74 and PvAvh8 coexist.…”
Section: Discussionmentioning
confidence: 99%
“…This effector, in addition, enhances tolerance to salinity and drought stress, extending the role of CRNs to the protection of plants from biotic and abiotic stresses ( Rajput et al, 2015 ). A very recent study has even shown that the virulence activity of the Phytophthora capsici CRN83_152 in planta is not related to its cell death inducing activity ( Amaro et al, 2018 ). All these results suggest that CRN proteins could have other functions besides cell death induction.…”
Section: Introductionmentioning
confidence: 99%