Abstract:Bacillus subtilis is an attractive host for directed evolution of the enzymes whose substrates cannot be transported across the cell membrane. However, generation of mutant library in B. subtilis still suffers problems of small library size, plasmid instability and heterozygosity. Here, large library of random mutant was created through inserting error-prone PCR (epPCR) product to the chromosome of B. subtilis. Specifically, epPCR product was fused with flanking regions and antibiotic resistant marker using a … Show more
“…We would like to acknowledge Dr. Daniel R. Zeigler from the BGSC for providing the B. subtilis SCK6 strain. This manuscript has been released as a pre-print at Authorea (Ye et al, 2020).…”
“…We would like to acknowledge Dr. Daniel R. Zeigler from the BGSC for providing the B. subtilis SCK6 strain. This manuscript has been released as a pre-print at Authorea (Ye et al, 2020).…”
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