Random amplified polymorphic DNA -RAPD analysis of human cervical cancer in Samwha city

Abstract

Objectives: The study intended to distinguish a likely hereditary variety in the genomic DNA of Iraqi patients corresponding with cervical disease and the possibility of getting an advantage of the potentially produced DNA fragment(s) as a molecular test for future related applications. Methods: Blood and tissue tests were collected from female patients with cervical malignant growth and solid females; DNA was extricated and purged from each example, and then "Irregular Amplification of Polymorphic DNA technique"(RAPD) was directed with oligonucleotide "OPA-20". Results: RAPD results examination with oligonucleotide “OPA-20” could identify four polymorphic DNA groups in the genome of patients females, in which these bands were missing in the majority of genomic DNA of healthy people. Keywords: Tumor, PCR, Iraq, gene polymorphism