Random amplified polymorphic DNA marker molecular and physiological characteristics in tomato plants induced by chemical mutagen

Abstract

Mutations are one method used to enhance the quality of tomato plants. Three distinct ethyl methane sulfonate (EMS) concentrations (0%, 0.3%, and 0.5%) and three potassium fertilizer doses (0 g, 2.5 g, and 5 g) per plant were used to treat local tomatoes. The complete genome DNA of immature tomato plant leaves was isolated using a DNA isolation kit. For DNA amplification, OPA primers 07, 08, 11, and 20 were applied. The analysis's findings utilizing RAPD demonstrated that genetic variation existed. The administration of EMS at various doses, specifically at 0.3% and 0.5% EMS without the inclusion of potassium, caused genetic modifications in the preliminary yield of tomato plants. Fertilizer and EMS had varied effects on lowering sugar and vitamin levels, total protein as well as fruit's Brix content. Different potassium dosages showed little impact on the genetic alterations in tomato plants. With four DNA samples amplified from four primers, the expression of those primers created two clusters. From a total of 12 DNA bands, the primers utilized produced a polymorphism percentage of 62.3%. There was no previously known process for the induction of mutations with concentrations.Previous research has been successful in developing RAPD markers gene mapping using tomato plants as a source of material [13][14][15]. Using the RAPD approach, some tomato cultivars have been thoroughly investigated [16,17]. Potassium is a nutrient that plants absorb in high quantities, particularly in tomato plants. Fruits and vegetables are