Random amplified polymorphic DNA for the specific detection of bubaline Echinococcus granulosus by hybridization assay

Reddy, Y.A.; Rao, J. R.; Butchaiah, G.; Sharma, Bhaskar

doi:10.1016/s0304-4017(98)00176-9

Cited by 14 publications

(10 citation statements)

References 18 publications

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“…These techniques are now being applied successfully to discriminate species and strains of E. granulosus (Lightowlers 1990;Thomson and Lymbery 1996). To date, there are nine distinct genotypes (G1-G9) of E. granulosus that have been identified using different techniques, such as the random-amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) and DNA sequence homology (Bowles and McManus 1993;McManus et al 1994;Reddy et al 1998). In addition, Gasser and Chilton (1995) used polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to identify camel and horse isolates of E. granulosus showing distinct enzymatic digestion sites.…”

Section: Introductionmentioning

confidence: 99%

Canine echinococcosis in northern Jordan: increased prevalence and dominance of sheep/dog strain

Al-Qaoud

Abdel‐Hafez

Craig³

2003

Parasitol Res

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A total of 112 stray and semi-stray dogs (Canis familiaris) from four different geographical areas in northern and middle Jordan were necropsied to evaluate the prevalence and intensity of intestinal helminthiasis. Of these, 33 dogs (29.5%) were infected with Echinococcus granulosus and 61 (54.5%) with other Taenia species. Other cestodes found included Dipylidium caninum in 36 dogs (32.1%), Diplopylidium in 6 dogs (5.4%), Mesocestoides sp. in 3 dogs (2.7%) and Joyuexiella in 1 dog (0.9%). Toxocara nematodes were found in 10 dogs (9.2%) and only 1 dog was positive for acanthocephalans. Among the dogs infected with E. granulosus, 8 dogs (24.2%) had a worm load higher than 1,000 worms. The ratio of infected male to female dogs was 1.9:1.0. Strain analysis of E. granulosus using random primers revealed the dominance of the G1 strain (sheep/dog strain) in the region. Only one dog harbored another E. granulosus strain, which resembled the G4 strain pattern.

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Section: Introductionmentioning

confidence: 99%

Canine echinococcosis in northern Jordan: increased prevalence and dominance of sheep/dog strain

Al-Qaoud

Abdel‐Hafez

Craig³

2003

Parasitol Res

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“…These variants were assigned to genotypes, as defined, e.g. according to mitochondrial gene sequencing (Bowles et al 1992;Bowles and McManus 1993), restriction fragment length polymorphism of the ribosomal ITS1 fragment (Bowles and McManus 1993) and comparative analyses of DNA sequence homologies (Bowles and McManus 1993;McManus et al 1994;Reddy et al 1998;Lavikainen et al 2003) In Bulgaria, the most important factors influencing the persistence, re-emergence and spread of E. granulosus infection have recently been described by Todorov and Boeva (1999). E. granulosus infection was highly endemic during the period between 1950 and 1962, with a total of 6,469 new surgical cases of CE in humans, equivalent to an annual incidence rate of 6.5 per 100,000 people.…”

Section: Introductionmentioning

confidence: 99%

Echinococcus granulosus strain typing in Bulgaria: the G1 genotype is predominant in intermediate and definitive wild hosts

Breyer

Georgieva

Kurdova

et al. 2004

Parasitology Research

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Addressing the genetic variability in Echinococcus granulosus is epidemiologically important, as strain characteristics may influence the local transmission patterns of zoonotic cystic echinococcosis. To classify the genotype(s) present in intermediate (pig, cattle and sheep) and definitive (jackal and wolf) hosts in Bulgaria, a DNA-based approach was used to assess parasite protoscoleces or strobiles. Genes corresponding to coding and non-coding regions of the nuclear and mitochondrial genome (ND-1, HBX, Act II, AgB-1) were amplified by PCR and subsequently sequenced. The sequences resolved were all found to be identical to those published for the common sheep strain of E. granulosus, indicating that the G1 genotype is predominant in Bulgaria. One microvariant for ND-1 was found in the pig isolates; however no epidemiological significance was attributed to this finding.

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“…For example, API and AP4 were found to generate high intense bands of 344 and 338 bp, respectively, which were more pronounced than other co-amplified DNA fragments. The more intense bands were probably due to priming within the repeated regions of the Fasciola genome, which could result in more copies being produced during PCR (MacPherson and Gajadhar 1993; Reddy et al 1998). Three possible explanations have been presented by Williams et al (1990) for the observation of polymorphic DNA fragments between or within species in the RAPD assays.…”

Section: Discussionmentioning

confidence: 99%

“…(Neto et al 1993), Echinococcus spp. (Siles-Lucas et al 1993;Reddy et al 1998), Trichinella spp. (Wu et al 1998), Echinostoma spp.…”

Section: Introductionmentioning

confidence: 99%

Elucidation of genetic variability among different isolates of Fasciola gigantica (giant liver fluke) using random-amplified polymorphic DNA polymerase chain reaction

Gunasekar¹,

Tewari

Sreekumar

et al. 2008

Parasitol Res

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The present communication focuses on molecular characterization of Fasciola gigantica isolates derived from cattle, buffalo, and goat using random-amplified polymorphic DNA (RAPD)-polymerase chain reaction (PCR) analysis to elucidate genetic variability between the three isolates. Seventeen random oligonucleotide primers of 10-11 bases with GC content varying from 50-81.8% were used in the study. Depending upon the F. gigantica isolate-primer combination, one to five fragments in the range of 327-1,973 bp were amplified. It was significant to observe that, out of the 17 primers directing amplification of DNA fingerprints, only two designated as AP9 and AP14 were found to be of potential interest in the generation of polymorphic DNA. On the basis of similarity coefficient data, it may be suggested that cattle and buffalo isolates of F. gigantica show 100% homogeneity against 92.68% similarity coefficient observed between goat and cattle/buffalo isolates. In other words, 7.32% divergence was observed between goat and cattle/buffalo isolates while the primers AP1, AP4, AP10, AP13, and AP17 were able to generate monomorphic DNA fingerprints. Primers AP9 and AP14 are potentially informative in terms of the polymorphic nature of the fingerprints generated in RAPD assays. The finding of the absence of 626-bp DNA fragment in the goat and the uniqueness of the AP14 in generating a single RAPD-PCR product of 1,211 bp as against the product size of 1,162 bp in cattle/buffalo seem to be significant. This is the first report of elucidation of RAPD-PCR based molecular variability in the DNA fingerprinting pattern of F. gigantica isolated from cattle, buffalo, and goat.

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Random amplified polymorphic DNA for the specific detection of bubaline Echinococcus granulosus by hybridization assay

Cited by 14 publications

References 18 publications

Canine echinococcosis in northern Jordan: increased prevalence and dominance of sheep/dog strain

Canine echinococcosis in northern Jordan: increased prevalence and dominance of sheep/dog strain

Echinococcus granulosus strain typing in Bulgaria: the G1 genotype is predominant in intermediate and definitive wild hosts

Elucidation of genetic variability among different isolates of Fasciola gigantica (giant liver fluke) using random-amplified polymorphic DNA polymerase chain reaction

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