Random amplified polymorphic DNA fingerprinting of Campylobacter jejuni and C. coli isolated from human faeces, seawater and poultry products

Hernández, Javier; Fayos, A.; Ferrús, María Antonia; Owen, Robert J.

doi:10.1016/0923-2508(96)81065-5

Cited by 53 publications

(39 citation statements)

References 29 publications

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“…The chromosomal DNA was separated by Random Amplified Polymorphic DNA (RAPD) method with using random primer OPA-11 5'-CAATCGCCGT-3' (Hernandez et al 1995). The results were interpreted using GeneTool (Syngene).…”

Section: Methodsmentioning

confidence: 99%

Prevalence of Campylobacter jejuni and Campylobacter coli species in cats and dogs from Bydgoszcz (Poland) region

Andrzejewska¹,

Szczepańska²,

Klawe³

et al. 2013

Polish Journal of Veterinary Sciences

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The aim of this study was to investigate the role of cats and dogs as a potential reservoir of Campylobacter spp. Rectal swabs from 83 dogs and 71 cats were examined. Samples were obtained from the animals aged between 2 weeks and 24 months living in shelters, private households, farms and from veterinary clinics located in Bydgoszcz region during routine check-up.Campylobacter spp. were isolated from 4.81% dogs and 9.86% cats, respectively. C. jejuni was predominant in this study. All strains were isolated in autumn and winter from the animals living in farms and private houses. All the animals positive for Campylobacter prevalence had access to small water basins, accidental source of food and had contact with wild birds, poultry or their feaces.Isolates characterization revealed high prevalence of Campylobacter virulence genes-flaA, cadF and cdtB. 91% of isolated strains were susceptible to erythromycin. 81% among isolated strains were susceptible to azithromycin, 64% to tetracycline and 36% to ciprofloxacin. For 2 C. jejuni strains isolated from cats Random Amplified Polymorphic DNA (RAPD) profiling indicated 80% homology between them.

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Section: Methodsmentioning

confidence: 99%

Prevalence of Campylobacter jejuni and Campylobacter coli species in cats and dogs from Bydgoszcz (Poland) region

Andrzejewska¹,

Szczepańska²,

Klawe³

et al. 2013

Polish Journal of Veterinary Sciences

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“…Using RAPD-PCR, we analyzed 8 isolates having serotype B and NA-CPFXresistant C. jejuni of which 5 isolates were from humans and 3 from poultry, and 7 isolates having serotype C and NA-CPFX-TC-resistant C. jejuni of which 4 isolates were from humans and 3 from poultry. We used InstaGene matrix (BioRad, Hercules, Calif., U.S.A.) for extracting the DNA from C. jejuni strains and OPA-11 primer, 5'-CAATCGCCGT-3' for amplifying the DNA (11). The template DNA (1 µl) was subjected to PCR in a 25 µl (total volume) reaction mixture containing 5 µl of 5 ϫ Flexi Buffer (Promega, Madison, Wis., U.S.A.), 3 µl of MgCl 2 (25 mM), 2.5 µl of dNTPs (1.25 mM of each dNTP), 0.2 µl of Taq polymerase (5 units/µl, Promega), 1 µl of OPA-11 primer (30 pmol), and 12.3 µl of DNase-and RNase-free distilled water.…”

mentioning

confidence: 99%

Serotypes, Antimicrobial Susceptibility, and gyr A Gene Mutation of Campylobacter jejuni Isolates from Humans and Chickens in Thailand

Boonmar

Morita

Fujita

et al. 2007

Microbiology and Immunology

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In Thailand, 51% (36/70) Campylobacter jejuni isolates from humans and 68% (47/69) isolates from poultry were classified into 10 Penner serotypes (serotype B, C, R, E, G, A, K, D, I, and L) and 9 serotypes (serotype A, C, I, K, B, E, S, D, and L), respectively. The rate of antimicrobial drug resistance to nalidixic acid, ciprofloxacin, ampicillin, tetracycline, and erythromycin shown by human isolates were 96%, 96%, 29%, 57%, and 14%, while that shown by poultry isolates were 77%, 77%, 22%, 26%, and 17%, respectively. All quinolone‐resistant strains contained a mutation in the gyrA gene (T86→I86), suggesting that the strains were already widespread in Thailand.

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“…In total, 87 different types (42 from cattle and 45 from sheep) were defined in this analysis of 348 Campylobacter isolates of various origins. In a previous study that employed the same primer, the number of RAPD types obtained in the examination of 208 samples was reported to be even higher (n=118) (Hernandez et al, 1995). This might be expected as they were testing isolates from various sources, and in the current paper the isolates came from multiple sites in two ruminant species.…”

Section: Resultsmentioning

confidence: 79%

“…For the RAPD analysis of C. jejuni and C. coli isolates, the reaction mixture was prepared in a total volume of 50 ml, consisting of 5 ml DNA, 106 PCR buffer (750 mM Tris/HCl, 200 mM (NH 4 ) 2 SO 4 , 0?1 % Tween 20), 3?5 mM MgCl 2 , 200 mM deoxynucleoside triphosphates, 1?25 U Taq DNA polymerase (MBI Fermentas) and 1 mM OPA-11 primer (59-CAA TCG CCG T-39) (Hernandez et al, 1995). The RAPD assay was performed in a thermal cycler with an initial denaturation step at 94 uC for 1 min, followed by 45 cycles at 94 uC for 1 min, 36 uC for 1 min and 72 uC for 2 min, then, a last step of extension at 72 uC for 5 min.…”

Section: Methodsmentioning

confidence: 99%

“…An RAPD assay that is based on the amplification of random DNA fragments using a single primer of arbitrary sequence was developed for Campylobacter spp. (Welsh & McClelland, 1990;Hernandez et al, 1995), and has been reported to be of great asset in establishing genetic diversity among C. jejuni and C. coli isolates from different sources (Hilton et al, 1997). RAPD analysis of C. jejuni and C. coli has frequently used 10-mer primers (Madden et al, 1996;Ertas et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Random amplified polymorphic DNA analysis of Campylobacter jejuni and Campylobacter coli isolated from healthy cattle and sheep

Açık

Çetinkaya

2006

Journal of Medical Microbiology

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The genetic heterogeneity among Campylobacter jejuni and Campylobacter coli isolates obtained from apparently healthy cattle and sheep was investigated by random amplified polymorphic DNA (RAPD) analysis. A total of 348 Campylobacter isolates, consisting of C. jejuni (n=218) and C. coli (n=130), were analysed. All these isolates were successfully typed by RAPD analysis. The total numbers of band patterns defined by RAPD in cattle and sheep were 42 and 45, respectively. Of the 42 distinct types obtained from cattle, 37 types were observed in C. jejuni isolates (n=115), and the remaining 5 were in C. coli isolates (n=30). Of 45 distinct types obtained from sheep, 21 types were observed in C. jejuni isolates (n=103), and 24 were in C. coli isolates (n=100). It was concluded that a high degree of heterogeneity existed among the C. jejuni and C. coli isolates of healthy cattle and sheep.

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Random amplified polymorphic DNA fingerprinting of Campylobacter jejuni and C. coli isolated from human faeces, seawater and poultry products

Cited by 53 publications

References 29 publications

Prevalence of Campylobacter jejuni and Campylobacter coli species in cats and dogs from Bydgoszcz (Poland) region

Prevalence of Campylobacter jejuni and Campylobacter coli species in cats and dogs from Bydgoszcz (Poland) region

Serotypes, Antimicrobial Susceptibility, and gyr A Gene Mutation of Campylobacter jejuni Isolates from Humans and Chickens in Thailand

Random amplified polymorphic DNA analysis of Campylobacter jejuni and Campylobacter coli isolated from healthy cattle and sheep

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