Raman Spectroscopy Study of Specific DNA Binding By Bis-Netropsins

Kryukov, E.; Ermishov, Mikhail A.; Sukhanova, Anna; Oleinikov, Vladimir; Grokhovsky, Sergey; Жузе, А. Л.; Jardillier, Jean‐Claude; Nabiev, Igor

doi:10.1007/978-94-011-4479-7_133

Cited by 2 publications

(3 citation statements)

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“…The doublet at 1404/1444 cm −1 , which can be used for evaluation of the access of the molecule to the SERS‐active surface,30 is particularly illustrative in a comparison of Raman and SERS spectra. For example, the ratio of the bands at 1404 and 1444 cm −1 is 0.5 in the Raman spectrum and 0.9 in the SERS spectrum.…”

Section: Resultsmentioning

confidence: 99%

“…For example, the ratio of the bands at 1404 and 1444 cm −1 is 0.5 in the Raman spectrum and 0.9 in the SERS spectrum. An increase in the intensity of the CN vibration of the pyrrole ring at 1404 cm −1 relative to the ring vibration at 1444 cm −1 ( I 1404 / I 1444 ) should be determined by reorientation of bis‐Nt molecules upon interaction with the SERS‐active surface 30. Consequently, this parameter ( I 1404 / I 1444 ) may be used for identification of the orientation of the pyrrole rings of bis‐Nts in their complexes with DNA.…”

Section: Resultsmentioning

confidence: 99%

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Raman and surface-enhanced Raman scattering spectroscopy of bis-netropsins and their DNA complexes

et al. 2000

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The interactions of three bis‐netropsins (bis‐Nts), which are potent catalytic inhibitors of DNA‐binding enzymes, with three double‐stranded oligonucleotides (OLIGs), which contain sites of different specific affinities for each bis‐Nt, were analyzed. Raman spectroscopy was performed for selective monitoring of modifications of the bis‐Nt or the OLIG structure upon bis‐Nt–DNA binding, and surface‐enhanced Raman scattering spectroscopy (SERS) was an additional tool for topology studies of ligand–DNA complexes. The spectral data showed conformational changes of both partners (bis‐Nt and OLIG) upon complexation. Structural variations of bis‐Nts appeared to be dependent on a bis‐Nt–OLIG binding constant and were found to be small in the specific DNA binding and highest for nonspecific binding of bis‐Nt with the corresponding OLIG. The conformational changes of the OLIGs were varied with a bis‐Nt–OLIG binding constant in the same manner. The bis‐Nts seemed to induce a perturbation in the OLIG's structure, as well as in the positions of their direct binding. These DNA structural modification effects may explain the inhibition of DNA‐binding enzymes in the variety of very distinct DNA–enzyme binding sites by bis‐Nts reported previously. © 2000 John Wiley & Sons, Inc. Biopolymers (Biospectroscopy) 57: 272–281, 2000

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Raman and surface-enhanced Raman scattering spectroscopy of bis-netropsins and their DNA complexes

et al. 2000

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“…Surface enhanced resonance Raman scattering, SERRS, has recently been developed for a wide number of analytical applications including detection of labelled DNA, [1][2][3][4] analysis of DNA groove binders, [5][6][7][8] analysis of porphyrins [9][10][11] and as an HPLC detection technique. 12 For a compound to be SERRS active it must adsorb onto the metal surface used to provide the surface enhancement and have an electronic transition with the frequency approximately coincident with the excitation frequency.…”

Section: Introductionmentioning

confidence: 99%