Raman Spectroscopy of Microbial Pigments

Jehlička, Jan; Edwards, Howell G. M.; Oren, Aharon

doi:10.1128/aem.00699-14

Cited by 148 publications

(125 citation statements)

References 126 publications

(129 reference statements)

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“…Recently, Raman spectroscopy was used extensively to monitor the spectroscopic and structural information of various pigments such as chlorophylls, carotenoids, and scytonemin found in the microbial samples including cyanobacteria [47,48]. Moreover, several other derivatives based on the scytoneman nucleus may be expected from different cyanobacteria.…”

Section: Westiliopsis Prolificamentioning

confidence: 99%

Cyanobacterial Sunscreen Scytonemin: Role in Photoprotection and Biomedical Research

Rastogi

Sonani

Madamwar

2015

Appl Biochem Biotechnol

107

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Cyanobacteria are the most promising group of photosynthetic microorganisms capable of producing an array of natural products of industrial importance. Scytonemin is a small hydrophobic alkaloid pigment molecules present in the extracellular sheath of several cyanobacteria as a protective mechanism against short wavelength solar ultraviolet (UV) radiation. It has great efficacy to minimize the production of reactive oxygen species and formation of DNA lesions. The biosynthesis of scytonemin is regulated by different physico-chemical stressors. Scytonemin display multiple roles, functioning as a potent UV sunscreen and antioxidant molecules, and can be exploited in cosmetic and other industries for the development of new cosmeceuticals. Herein, we review the occurrence, biosynthesis, and potential application of scytonemin in photoprotection, pharmaceuticals, and biomedical research.

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Section: Westiliopsis Prolificamentioning

confidence: 99%

Cyanobacterial Sunscreen Scytonemin: Role in Photoprotection and Biomedical Research

Rastogi

Sonani

Madamwar

2015

Appl Biochem Biotechnol

107

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“…Introduction origin can be followed in the frame of native colonizations or cultivated microorganisms with advantage using Raman spectroscopy (see mini review by Jehlička et al [50]). The advantage consists of the fast and direct mode of analysis of the biomass or stone colonization.…”

mentioning

confidence: 99%

Miniaturized Raman instrumentation detects carotenoids in Mars-analogue rocks from the Mojave and Atacama deserts

Vítek

Jehlička

Edwards

et al. 2014

Phil. Trans. R. Soc. A.

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This study is primarily focused on proving the potential of miniaturized Raman systems to detect any biomolecular and mineral signal in natural geobiological samples that are relevant for future application of the technique within astrobiologically aimed missions on Mars. A series of evaporites of varying composition and origin from two extremely dry deserts were studied, namely Atacama and Mojave. The samples represent both dry evaporitic deposits and recent evaporitic efflorescences from hypersaline brines. The samples comprise halite and different types of sulfates and carbonates. The samples were analysed in two different ways: (i) directly as untreated rocks and (ii) as homogenized powders. Two excitation wavelengths of miniaturized Raman spectrometers were compared: 532 and 785 nm. The potential to detect carotenoids as biomarkers on Mars compared with the potential detection of carbonaceous matter using miniaturized instrumentation is discussed.

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“…This method is ideally applicable for the Raman analysis of samples containing pigments like carotenoids using the 514 and 532 nm lasers as excitation source. This wavelength range mostly falls within the electronic absorption of most of the carotenoids resulting in resonance Raman enhancement of carotenoid signatures overlapping all other biomolecular signatures (Collins et al 2011;Jehlička et al 2014). There have been previous studies wherein an extended multiplicative scatter correction and spectral interference subtraction (EMSC-SIS) algorithm was used to remove the contribution of pigments in the spectra of five species of pigmented bacteria; however, these studies were performed using a laser of 785 nm excitation wavelength wherein the resonance conditions for carotenoids in the sample is not met and the Raman bands of other biomolecules are partly visible even at the first measurements (Scholtes-Timmerman et al 2009).…”

Section: Resultsmentioning

confidence: 98%

Classification and identification of pigmented cocci bacteria relevant to the soil environment via Raman spectroscopy

Kumar

Kampe

Rösch

et al. 2015

Environ Sci Pollut Res

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A soil habitat consists of a significant number of bacteria that cannot be cultivated by conventional means, thereby posing obvious difficulties in their classification and identification. This difficulty necessitates the need for advanced techniques wherein a well-compiled biomolecular database consisting of the already cultivable bacteria can be used as a reference in an attempt to link the noncultivable bacteria to their closest phylogenetic groups. Raman spectroscopy has been successfully applied to taxonomic studies of many systems like bacteria, fungi, and plants relying on spectral differences contributed by the variation in their overall biomolecular composition. However, these spectral differences can be obscured due to Raman signatures from photosensitive microbial pigments like carotenoids that show enormous variation in signal intensity hindering taxonomic investigations. In this study, we have applied laser-induced photobleaching to expel the carotenoid signatures from pigmented cocci bacteria. Using this method, we have investigated 12 species of pigmented bacteria abundant in soil habitats belonging to three genera mainly Micrococcus, Deinococcus, and Kocuria based on their Raman spectra with the assistance of a chemometric tool known as the radial kernel support vector machine (SVM). Our results demonstrate the potential of Raman spectroscopy as a minimally invasive taxonomic tool to identify pigmented cocci soil bacteria at a single-cell level.

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Raman Spectroscopy of Microbial Pigments

Cited by 148 publications

References 126 publications

Cyanobacterial Sunscreen Scytonemin: Role in Photoprotection and Biomedical Research

Cyanobacterial Sunscreen Scytonemin: Role in Photoprotection and Biomedical Research

Miniaturized Raman instrumentation detects carotenoids in Mars-analogue rocks from the Mojave and Atacama deserts

Classification and identification of pigmented cocci bacteria relevant to the soil environment via Raman spectroscopy

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