Raman Spectroscopy of Biological Tissues

Movasaghi, Zanyar; Rehman, Shazza; Rehman, Ihtesham Ur

doi:10.1080/05704920701551530

Cited by 2,034 publications

(1,981 citation statements)

References 82 publications

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“…Figure 4 shows the peak intensity maps of (a) the Amide I (C ő O stretching vibration; 1669 cm -1 (Movasaghi et al, 2007)), which mainly corresponds to the collagenous matrix (Kazanci et al, 2007), (b) the Ȟ 1 PO 4 vibrational mode of the mineral compound (960 cm -1 ), (c) the C-H -bending (1458 cm í1 ), which corresponds to both collagen and NCPs (Kazanci et al, 2007) and finally (d) the ratio of Amide I / C-H -bending.…”

Section: Interlamellar Areas Are Collagen-deficient and Ncp-richmentioning

confidence: 99%

Load-bearing in cortical bone microstructure: Selective stiffening and heterogeneous strain distribution at the lamellar level

Katsamenis

Chong

Andriotis

et al. 2013

Journal of the Mechanical Behavior of Biomedical Materials

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An improved understanding of bone mechanics is vital in the development of evaluation strategies for patients at risk of bone fracture. The current evaluation approach based on bone mineral density (BMD) measurements lacks sensitivity, and it has become clear that as well as bone mass, bone quality should also be evaluated.The latter includes, among other parameters, the bone matrix material properties, which in turn depend on the hierarchical structural features that make up bone as well as their composition. Optimal load transfer, energy dissipation and toughening mechanisms have, to some extent, been uncovered in bone. Yet, the origin of these properties and their dependence upon the hierarchical structure and composition of bone are largely unknown. Here we investigate load transfer in the osteonal and subosteonal levels and the mechanical behaviour of osteonal lamellae and interlamellar areas during loading. Using cantilever-based nanoindentation, in situ microtensile testing during atomic force microscopy (AFM) and digital image correlation (DIC), we report evidence for a previously unknown mechanism. This mechanism transfers load and movement in a manner analogous to the engineered "elastomeric bearing pads" used in large engineering structures. ȝ-RAMAN microscopy investigations 2 showed compositional differences between lamellae and interlamellar areas. The latter have lower collagen content but an increased concentration of noncollagenous proteins (NCPs). Hence, NCPs enriched areas on the microscale might be similarly important for bone failure as on the nanoscale. Finally, we managed to capture stable crack propagation within the interlamellar areas in a time-lapsed fashion, proving their significant contribution towards fracture toughness.

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Section: Interlamellar Areas Are Collagen-deficient and Ncp-richmentioning

confidence: 99%

Load-bearing in cortical bone microstructure: Selective stiffening and heterogeneous strain distribution at the lamellar level

Katsamenis

Chong

Andriotis

et al. 2013

Journal of the Mechanical Behavior of Biomedical Materials

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“…Conversely, apart from the advantage of being able to use FT techniques with 1064-nm excitation, the lack of fluorescence, which often accompanies visible irradiation with biological samples and swamps the Raman spectrum, is an additional advantage. These advantages combined often outweigh the disadvantages and have resulted in the extensive use of FT-Raman studies on biological samples (Gierlinger and Schwanninger 2007;Moreira et al 2008;Movasaghi et al 2007;Parker 1994).…”

Section: Raman Spectroscopic Microprobesmentioning

confidence: 99%

“…Because of the rapid expansion of biological applications of vibrational spectroscopic mapping and imaging techniques, it is not possible to provide an extensive review of recent research within the space limitations of this article, but an overview of the diversity of applications in medicine and biology, including medical diagnostics, studies of physiological and diseases processes and treatments of diseases and plant biology, can be found in a number of recent reviews (Bailo and Deckert 2008;Bhargava 2007;Boskey and Mendelsohn 2005;Chan et al 2008;Gierlinger and Schwanninger 2007;Levin and Bhargava 2005;Moreira et al 2008;Movasaghi et al 2007;Petibois and Guidi 2008;Petter et al 2009;Srinivasan and Bhargava 2007;Swain and Stevens 2007). As outlined in these reviews and the current review, the increase in the speed of acquisition, sensitivity, discrimination and spatial resolution of the emerging techniques of vibrational spectroscopic microscopies has greatly expanded the horizons of what is possible in terms of an explosion of new knowledge that is being generated.…”

Section: Applications To Biological Systemsmentioning

confidence: 99%

Vibrational spectroscopic mapping and imaging of tissues and cells

et al. 2009

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“…By using light to interrogate a sample, and seeking to correlate a change in the observed optical signal to a biological phenomenon, optical methods allow minimally-invasive measurements to be performed on sensitive samples. Different sensing modalities have been utilized for measurements of biologically relevant parameters, such as fluorescence sensing (Tsiminis et al 2014), resonant techniques such as surface plasmon resonance (Francois et al 2011), Bragg gratings which can be utilized either for biological 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 sensing or physical parameters (Warren-Smith & Monro 2014), or alternative label-free methods such as Raman spectroscopy (Movasaghi et al 2007). …”

Section: Raman Spectroscopymentioning

confidence: 99%

Novel imaging tools for investigating the role of immune signalling in the brain

Jacobsen

Parker

Everest‐Dass

et al. 2016

Brain, Behavior, and Immunity

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In all cases accepted manuscripts should:  link to the formal publication via its DOI  bear a CC-BY-NC-ND license -this is easy to do, click here to find out how  if aggregated with other manuscripts, for example in a repository or other site, be shared in alignment with our hosting policy  not be added to or enhanced in any way to appear more like, or to substitute for, the published journal article AbstractThe importance of neuro-immune interactions in both physiological and pathophysiological states cannot be understated. As our appreciation for the neuroimmune nature of the brain and spinal cord grows, so does our need to extend the spatial and temporal resolution of our molecular analysis techniques. Current imaging technologies applied to investigate the actions of the neuroimmune system in both health and disease states have been adapted from the fields of immunology and neuroscience and are inherently limited by their semiquantitative nature, loss of spatial resolution, photobleaching and detection sensitivity. Thus, the development of innovative methods which overcome these limitations are crucial for imaging and quantifying acute and chronic neuroimmune responses. Therefore, this review aims to convey novel and complementary imaging technologies in a form accessible to medical scientists engaging in neuroimmune research.

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Raman Spectroscopy of Biological Tissues

Cited by 2,034 publications

References 82 publications

Load-bearing in cortical bone microstructure: Selective stiffening and heterogeneous strain distribution at the lamellar level

Load-bearing in cortical bone microstructure: Selective stiffening and heterogeneous strain distribution at the lamellar level

Vibrational spectroscopic mapping and imaging of tissues and cells

Novel imaging tools for investigating the role of immune signalling in the brain

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