Raman spectroscopy for osteoarthritis severity and cartilage degradation assessment - defining optical biomarkers using an ex vivo model

Casal-Beiroa, Paula; Balboa‐Barreiro, Vanesa; Pértega-Díaz, Sonia; González, P.; Oreiro, N.; Blanco, F.J.; Magalhães, Joana

doi:10.1016/j.joca.2020.02.504

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“…Other ratios could be considered to assess cartilage quality and tissue mineralization processes, such as cartilage to bone (1063/958 cm -1 ), bone to collagen (960/920 cm -1 ), and carbonate to phosphate (1070/958 cm -1 ) ratios [27]. Some of these ratios have been lately validated against K-L grade, Mankin Score and biochemical assays [97]. Concomitantly, changes in bone during OA could be detected by hydroxyapatite to collagen (960/1245 cm -1 and 960/1270 cm -1 ), carbonate to phosphate (1070/960 cm -1 ) and collagen randomness (1270/1245 cm -1 ) ratios.…”

Section: Rs For Oa Candidate Biomarkersmentioning

confidence: 99%

Molecular analysis of the destruction of articular joint tissues by Raman spectroscopy

Casal-Beiroa

González

Blanco

et al. 2020

Expert Review of Molecular Diagnostics

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Introduction: Osteoarthritis (OA) is a highly heterogenous disease influenced by different molecular, anatomic and physiologic imbalances. Some of the bottlenecks for enhanced diagnosis and therapeutic assessment are the lack of validated biomarkers and early diagnosis tools. In this narrative review, we analyze the potential of Raman spectroscopy (RS) as a label-free optical tool for the characterization of articular joint tissues and its application as a diagnosis tool for OA.Areas covered: Raman spectra produce a unique "molecular fingerprint" providing rotational and vibrational molecular information, allowing the identification and follow-up of molecular changes associated with OA pathological mechanisms. Focusing on multiple joint tissues (cartilage, synovium, bone, tendons, ligaments and meniscus) and their contribution in disease incidence and progression, this review highlights the current knowledge on the application of RS in the characterization of organic and inorganic molecules present at these tissues and alterations that occur in the onset of OA.Expert Opinion: Vibrational spectroscopy techniques, such as RS, are low cost, rapid and minimally invasive approaches that offer high specificity in the assessment of the molecular composition of complex tissues. Combined with multivariate statistical methods, RS offers great potential for optical biomarkers discovery or disease diagnosis applications, and we hereby discuss clinical translational progresses on the field.

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Section: Rs For Oa Candidate Biomarkersmentioning

confidence: 99%