Raman spectroscopy enables noninvasive biochemical identification of the collagen regeneration in cutaneous wound healing of diabetic mice treated with MSCs

Abstract

Mesenchymal stem cells (MSCs) had been reported as a novel therapeutic strategy for non-healing diabetic cutaneous wound mainly by promoting the formation of extracellular matrix (ECM) and neovasculature. Collagen regeneration is one of the key processes of ECM remodeling in wound healing. Accordingly, rapid assessment of the collagen content in a noninvasive manner can promptly provide objective evaluation for MSC therapy of cutaneous wound healing and strength evidence to adjust therapeutic regimen. In the present study, noninvasive Raman microspectroscopy was used for tracing the regeneration status of collagen during diabetic wound healing with MSCs. Wound tissues of normal mice, diabetic mice, and MSC-treated diabetic mice were subjected to Masson trichrome staining assay and submitted to spectroscopic analysis by Raman microspectroscopy after wounding 7, 14, and 21 days. Masson trichrome staining demonstrated that there was more collagen deposition in diabetic + MSCs group relative to diabetic group. The relative intensity of Raman collagen peak positions at 937, 1004, 1321, 1452, and 1662 cm increased in MSC-treated diabetic group compared to diabetic group, although normal mice group had the highest relative intensity of collagen peak bands. Correlation analysis suggested that the spectral bands had a high positive correlation with the collagen intensity detected by Masson trichrome staining in wound tissues of three groups. Our results demonstrate that Raman microspectroscopy has potential application in rapidly and quantitatively assessing diabetic wound healing with MSCs by monitoring collagen variation, which may provide a novel method for the study of skin regeneration.

Use of Raman spectroscopy in the assessment of skin after CO2ablative fractional laser surgery on acne scars

“…All nine patients completed the treatment with a remarkable reduction in acne scarring, and a wound healing of AFL induced injury without acne relapse. Raman spectrum of type 1 collagen (Figure ) used as a reference shows six characteristic bands (Table ) with intensities related to collagen and previously reported …”

“…Type 1 collagen is the most abundant component of human skin (about 80% of total collagen content in adult skin), and it is well established that a variation of its presence could be used as a biomarker for the assessment of skin regeneration . In this work, the presence of type 1 collagen is assessed noninvasively by Raman spectroscopy in order to evaluate the wound healing process of patients after being exposed to the ablative fractional laser surgery.…”

Wound Healing Process After Thermomechanical Skin Ablation

“…A limitation when using CLSM is incapability for measuring the collagen content in the skin. Collagen is the most abundant protein in human skin and it has been described to be possibly used as biomarker for skin regeneration 14. Moreover, this proof‐of‐concept study lacks information about cytokine levels and expression of heat shock proteins and other factors that affect wound‐healing and have already been examined following CO 2 fractional laser ablation 15.…”

Mesenchymal Stem Cell Therapies for Skin Repair and Regeneration

“…Bone marrow MSCs have been explored as a treatment option [15][16][17]. In one study, allogeneic MSCs were compared to the MSCs' acellular derivatives in a non-obese diabetic (NOD) mouse model [15].…”

“…Bone marrow MSCs have been known to differentiate into a wide variety of mesenchymal cell types, including bone, cartilage and fat cells [9-11] and even into cells beyond its originating phenotype, such as cardiomyocytes and neurons [12,13]. In applications to the skin, bone marrow aspirate, bone marrow mononuclear cells, and a combination of cells and biomaterial have been used [14][15][16][17]. In a study utilizing autologous bone marrow aspirate [14], 75 patients with chronic wounds were enrolled.…”