Raman Spectroscopy Enables Non-Invasive Identification of Mycotoxins p. Fusarium of Winter Wheat Seeds

Moskovskiy, Maksim; Sibirev, Alexey; Gulyaev, Anatoly; Gerasimenko, Stanislav; Borzenko, Sergey; Godyaeva, Maria; Noy, Oleg V.; Nagaev, Egor I.; Matveeva, Tatiana; Sarimov, Ruslan M.; Simakin, Alexander V.

doi:10.3390/photonics8120587

Cited by 6 publications

(2 citation statements)

References 28 publications

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“…Each sample was measured three times. The Figures show typical spectra: with repeated measurements, the intensity maxima changes by several percent [ 51 ].…”

Section: Methodsmentioning

confidence: 99%

Effect of Laser-Induced Optical Breakdown on the Structure of Bsa Molecules in Aqueous Solutions: An Optical Study

et al. 2022

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The influence of laser radiation of a typical surgical laser on the physicochemical properties of the Bovine Serum Albumin (BSA) protein was studied. It was established that the physicochemical characteristics of optical breakdown weakly depend on the concentration of protein molecules. At the same time, the patterns observed for an aqueous solution of BSA irradiated with a laser for different time periods were extremely similar to the classical ones. It was established that after exposure to laser radiation, the optical density of protein solutions increases. At the same time, the intensity of BSA fluorescence due to aromatic amino acid residues decreases insignificantly after exposure to laser radiation. In this case, the position of the excitation and emission maximum does not change, and the shape of the fluorescence spot on 3D maps also does not change significantly. On the Raman spectrum after exposure to laser radiation, a significant decrease in 1570 cm−1 was observed, which indicates the degradation of α-helices and, as a result, partial denaturation of BSA molecules. Partial denaturation did not significantly change the total area of protein molecules, since the refractive index of solutions did not change significantly. However, in BSA solutions, after exposure to laser radiation, the viscosity increased, and the pseudoplasticity of aqueous solutions decreased. In this case, there was no massive damage to the polypeptide chain; on the contrary, when exposed to optical breakdown, intense aggregation was observed, while aggregates with a size of 400 nm or more appeared in the solution. Thus, under the action of optical breakdown induced by laser radiation in a BSA solution, the processes of partial denaturation and aggregation prevail, aromatic amino acid residues are damaged to a lesser extent, and fragmentation of protein molecules is not observed.

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“…Each sample was measured three times. The Figures show typical spectra: with repeated measurements, the intensity maxima changes by several percent [ 51 ].…”

Section: Methodsmentioning

confidence: 99%

Effect of Laser-Induced Optical Breakdown on the Structure of Bsa Molecules in Aqueous Solutions: An Optical Study

et al. 2022

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“…Some of the general applications of Raman spectroscopy include the detection of crop diseases and toxin components as well as the evaluation of structural and compositional analysis of agricultural products (Nadimi et al., 2021b). In the case of intact wheat, the applications of Raman spectroscopy have been shown on fungi and mycotoxin detection (Egging et al., 2018; Jia et al., 2020; Moskovskiy et al., 2021; Pandiselvam et al., 2021) and protein quality evaluation (Kłosok et al., 2021; Stawoska et al., 2021). MIR spectroscopy is often used for the quality evaluation of grains.…”

Section: Measurement Techniquesmentioning

confidence: 99%

Enhancing traceability of wheat quality through the supply chain

Nadimi

Hawley²,

Liu

et al. 2023

Comp Rev Food Sci Food Safe

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With the growing global population, the need for food is expected to grow tremendously in the next few decades. One of the key tools to address such growing food demand is minimizing grain losses and optimizing food processing operations. Hence, several research studies are underway to reduce grain losses/degradation at the farm (upon harvest) and later during the milling and baking processes. However, less attention has been paid to changes in grain quality between harvest and milling. This paper aims to address this knowledge gap and discusses possible strategies for preserving grain quality (for Canadian wheat in particular) during unit operations at primary, process, or terminal elevators. To this end, the importance of wheat flour quality metrics is briefly described, followed by a discussion on the effect of grain properties on such quality parameters. This work also explores how drying, storage, blending, and cleaning, as some of the common post-harvest unit operations, could affect grain's end-product quality. Finally, an overview of the available techniques for grain quality monitoring is provided, followed by a discussion on existing gaps and potential solutions for quality traceability throughout the wheat supply chain.

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The Effect of Surgical Laser Radiation on the Structure of Bovine Serum Albumin in vitro

Serov,

Nagaev,

Kuleshova

et al. 2023

BIOPHYSICS

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Raman Spectroscopy Enables Non-Invasive Identification of Mycotoxins p. Fusarium of Winter Wheat Seeds

Cited by 6 publications

References 28 publications

Effect of Laser-Induced Optical Breakdown on the Structure of Bsa Molecules in Aqueous Solutions: An Optical Study

Effect of Laser-Induced Optical Breakdown on the Structure of Bsa Molecules in Aqueous Solutions: An Optical Study

Enhancing traceability of wheat quality through the supply chain

The Effect of Surgical Laser Radiation on the Structure of Bovine Serum Albumin in vitro

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