Raman spectroscopy‐based detection of <scp>RNA</scp> viruses in saliva: A preliminary report

Desai, Sanket; Mishra, Saket V.; Joshi, Asim; Sarkar, Debashmita; Hole, Arti; Mishra, Rohit; Dutt, Shilpee; C, Murali Krishna; Gupta, Sudeep; Dutt, Amit

doi:10.1002/jbio.202000189

Cited by 46 publications

(47 citation statements)

References 15 publications

(16 reference statements)

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“…The technique offers an opportunity to assess the viral decay in the wastewater treatment plants and the tracking of SARS-CoV-2 in the pipe network. Viral RNA detection from saliva was also reported in a recent study using Raman spectroscopy with a sensitivity of 7.05 × 10 7 transduction units per ml viral particles ( Desai et al, 2020 ).…”

Section: Raman Spectroscopymentioning

confidence: 69%

Optical technologies for the detection of viruses like COVID-19: Progress and prospects

Lukose

Chidangil

George

2021

Biosensors and Bioelectronics

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Section: Raman Spectroscopymentioning

confidence: 69%

Optical technologies for the detection of viruses like COVID-19: Progress and prospects

Lukose

Chidangil

George

2021

Biosensors and Bioelectronics

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“…Therefore, the authors believe that it is important to check the presence of SARS-CoV-2 in the tear sample along with other systematic fluids. A recent report from Tata Memorial Center (TMC) group showed the presence of RNA in saliva by using Raman Spectroscopy [14] . Thus, it is worth collecting and analysing tear and saliva sample along with the nasal and throat swabs for the detection of SARS-CoV-2.…”

Section: Statement To Hypothesismentioning

confidence: 99%

Development of integrated microfluidic platform coupled with Surface-enhanced Raman Spectroscopy for diagnosis of COVID-19

et al. 2021

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Corona Virus Disease 19 (COVID-19) pandemic has created an alarming situation across the globe. Varieties of diagnostic protocols are being developed for the diagnosis of COVID-19. Many of these diagnostic protocols however, have limitations such as for example unacceptable no of false-positive and false-negative cases, particularly during the early stages of infection. At present, the real-time (quantitative) reverse transcriptase-polymerase chain reaction (RT-PCR) is considered the gold standard for COVID-19 diagnosis. However, RT-PCR based tests are complex, expensive, time consuming and involve pre-processing of samples. A swift, sensitive, inexpensive protocol for mass screening is urgently needed to contain this pandemic. There is urgent need to harness new powerful technologies for accurate detection not only of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) but also combating the emergence of pandemics of new viruses as well. To overcome the current challenges, the authors propose a diagnostic protocol based on surface-enhanced Raman Spectroscopy (SERS) coupled with microfluidic devices containing integrated microchannels functionalized either with vertically aligned Au/Ag coated carbon nanotubes or with disposable electrospun micro/nano-filter membranes. These devices have the potential to successfully trap viruses from diverse biological fluids/secretions including saliva, nasopharyngeal, tear etc. These can thus enrich the viral titre and enable accurate identification of the viruses from their respective Raman signatures. If the device is successfully developed and proven to detect target viruses, it would facilitate rapid screening of symptomatic as well as asymptomatic individuals of COVID-19. This would be a valuable diagnostic tool not only for mass screening of current COVID -19 pandemic but also in viral pandemic outbreaks of future.

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“…They achieved a prediction accuracy of 91.6%. 46 However, despite its promise, little has been studied on the use of Raman spectroscopy to develop clinical tests for COVID-19 diagnostics.…”

Section: Omics-based Technology Platform For Covid-19 Researchmentioning

confidence: 99%

Role of Multiomics Data to Understand Host–Pathogen Interactions in COVID-19 Pathogenesis

et al. 2021

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Human infectious diseases are contributed equally by the host immune system’s efficiency and any pathogens’ infectivity. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the coronavirus strain causing the respiratory pandemic coronavirus disease 2019 (COVID-19). To understand the pathobiology of SARS-CoV-2, one needs to unravel the intricacies of host immune response to the virus, the viral pathogen’s mode of transmission, and alterations in specific biological pathways in the host allowing viral survival. This review critically analyzes recent research using high-throughput “omics” technologies (including proteomics and metabolomics) on various biospecimens that allow an increased understanding of the pathobiology of SARS-CoV-2 in humans. The altered biomolecule profile facilitates an understanding of altered biological pathways. Further, we have performed a meta-analysis of significantly altered biomolecular profiles in COVID-19 patients using bioinformatics tools. Our analysis deciphered alterations in the immune response, fatty acid, and amino acid metabolism and other pathways that cumulatively result in COVID-19 disease, including symptoms such as hyperglycemic and hypoxic sequelae.

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Raman spectroscopy‐based detection of RNA viruses in saliva: A preliminary report

Cited by 46 publications

References 15 publications

Optical technologies for the detection of viruses like COVID-19: Progress and prospects

Optical technologies for the detection of viruses like COVID-19: Progress and prospects

Development of integrated microfluidic platform coupled with Surface-enhanced Raman Spectroscopy for diagnosis of COVID-19

Role of Multiomics Data to Understand Host–Pathogen Interactions in COVID-19 Pathogenesis

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