Raman Spectroscopy as a Biomarker-Investigative Tool in Bone Metabolism

Bosser, C; Ogier, Agathe; Imbert, Laurianne; Hoc, Thierry

doi:10.1007/978-94-007-7693-7_31

Cited by 1 publication

(2 citation statements)

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“…For determining the quality of bone, physicochemical properties of mineralized crystals and the organic matrix were characterized by using Raman spectral analysis . Raman spectra of bone tissue samples collected from different treated groups are shown (Figure C–G).…”

Section: Resultsmentioning

confidence: 99%

“…For determining the quality of bone, physicochemical properties of mineralized crystals and the organic matrix were characterized by using Raman spectral analysis. 42 , respectively. Organic phase molecular species are detected at the following peaks: the peak at 1003 cm −1 represents phenylalanine ring breathing, while the peaks at 1246 and 1667 cm −1 can be attributed to the C−N−H stretch vibration of amide III and the C−C−N stretching vibration of amide I, respectively.…”

Section: αPdd and Rn1734 Containing Cmt-hy Surfaces Regulate Mitochon...mentioning

confidence: 97%

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TRPV4 Activator-Containing CMT-Hy Hydrogel Enhances Bone Tissue Regeneration In Vivo by Enhancing Mitochondrial Health

Kumar,

Acharya,

Kumar

et al. 2024

ACS Biomater. Sci. Eng.

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Treating different types of bone defects is difficult, complicated, time-consuming, and expensive. Here, we demonstrate that transient receptor potential cation channel subfamily V member 4 (TRPV4), a mechanosensitive, thermogated, and nonselective cation channel, is endogenously present in the mesenchymal stem cells (MSCs). TRPV4 regulates both cytosolic Ca 2+ levels and mitochondrial health. Accordingly, the hydrogel made from a natural modified biopolymer carboxymethyl tamarind CMT-Hy and encapsulated with TRPV4-modulatory agents affects different parameters of MSCs, such as cell morphology, focal adhesion points, intracellular Ca 2+ , and reactive oxygen species-and NO-levels. TRPV4 also regulates cell differentiation and biomineralization in vitro. We demonstrate that 4α-10-CMT-Hy and 4α-50-CMT-Hy (the hydrogel encapsulated with 4αPDD, 10 and 50 nM, TRPV4 activator) surfaces upregulate mitochondrial health, i.e., an increase in ATP-and cardiolipin-levels, and improve the mitochondrial membrane potential. The same scaffold turned out to be nontoxic in vivo. 4α-50-CMT-Hy enhances the repair of the bone-drill hole in rat femur, both qualitatively and quantitatively in vivo. We conclude that 4α-50-CMT-Hy as a scaffold is suitable for treating large-scale bone defects at low cost and can be tested for clinical trials.

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Section: Resultsmentioning

confidence: 99%

Section: αPdd and Rn1734 Containing Cmt-hy Surfaces Regulate Mitochon...mentioning

confidence: 97%