2002
DOI: 10.1002/cphc.200390004
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Raman Spectroscopy—A Prospective Tool in the Life Sciences

Abstract: Although the physics of Raman spectroscopy and its application to purely chemical problems is long established, it offers a noninvasive, nondestructive, and water-insensitive probe to problems in the life sciences. Starting from the principles of Raman spectroscopy, its advantages, and methods for signal enhancement, the bulk of the review highlights recent applications. Structural investigations of a hormone receptor, testing the biocompatibility of dental implants, probing soil components and plant tissue al… Show more

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Cited by 325 publications
(205 citation statements)
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“…This enables the ''visualisation'' of the vibrational frequencies within the molecule of interest and effective suppression of unwanted background signal. Intense fluorescence emission associated with complex samples usually occurs in the same spectral region as the resonance Raman spectra and irretrievably swamps weaker Raman signals (Petry et al 2003). The fluorescence problem can also be circumvented by probing well below the fluorescence emission in the ultraviolet (UV) (Halttunen et al 2001), an approach that offers also the benefit of a higher signal strength compared to NIR excitation.…”
Section: Principles Of Infrared Raman and Uv Microspectroscopy With mentioning
confidence: 99%
“…This enables the ''visualisation'' of the vibrational frequencies within the molecule of interest and effective suppression of unwanted background signal. Intense fluorescence emission associated with complex samples usually occurs in the same spectral region as the resonance Raman spectra and irretrievably swamps weaker Raman signals (Petry et al 2003). The fluorescence problem can also be circumvented by probing well below the fluorescence emission in the ultraviolet (UV) (Halttunen et al 2001), an approach that offers also the benefit of a higher signal strength compared to NIR excitation.…”
Section: Principles Of Infrared Raman and Uv Microspectroscopy With mentioning
confidence: 99%
“…Raman spectroscopy belongs to molecular vibration spectrum likes as infrared spectroscopy, and can reflect the characteristics of molecular structure. But Raman signal is very weak; the intensity of the incident light intensity is only about 10 -10 [26].…”
Section: Surface-enhanced Raman Scattering (Sers)mentioning
confidence: 99%
“…Kim et al [29] published high-harmonic spectra that they had produced in the traditional strong field process by making use of field enhancing plasmonics in metallic nano-structures. While the near limitless spatial shaping of optical near fields in plasmonics [30] has led to a fantastic range of fundamental scientific as well as real world applications [31,32], its combination with classical strong field effects is a very recent development [33,34]. A conceptual sketch of the experiment that led to the publication in [29] is shown in Fig.…”
Section: Theory Of Plasmon-assisted Hhgmentioning
confidence: 99%