Raman spectroscopy – A potential new method for the intra-operative assessment of axillary lymph nodes

Horsnell, Jonathan; Smith, J.; Sattlecker, Martina; Sammon, Alastair M.; Christie-Brown, Jonathan; Kendall, Catherine; Stone, Nick

doi:10.1016/j.surge.2011.02.004

Cited by 44 publications

(33 citation statements)

References 20 publications

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“…Among the four standards in the study, if a node was classified based on the simple majority of the five random spectra, the sensitivity and specificity were higher than those in the other groups. Thereafter, to study the effect of the number of spectra, Horsnell et al compared five-and 10-point probe analyses [40]. The assessment of the lymph node based on spectra from five points achieved 71% [42,43].…”

Section: Raman Spectroscopy In Tissuesmentioning

confidence: 99%

The Clinical Application of Raman Spectroscopy for Breast Cancer Detection

Gao

Han

et al. 2017

Journal of Spectroscopy

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Raman spectroscopy has been widely used as an important clinical tool for real-time in vivo cancer diagnosis. Raman information can be obtained from whole organisms and tissues, at the cellular level and at the biomolecular level. The aim of this paper is to review the newest developments of Raman spectroscopy in the field of breast cancer diagnosis and treatment. Raman spectroscopy can distinguish malignant tissues from noncancerous/normal tissues and can assess tumor margins or sentinel lymph nodes during an operation. At the cellular level, Raman spectra can be used to monitor the intracellular processes occurring in blood circulation. At the biomolecular level, surface-enhanced Raman spectroscopy techniques may help detect the biomarker on the tumor surface as well as evaluate the efficacy of anticancer drugs. Furthermore, Raman images reveal an inhomogeneous distribution of different compounds, especially proteins, lipids, microcalcifications, and their metabolic products, in cancerous breast tissues. Information about these compounds may further our understanding of the mechanisms of breast cancer.

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Section: Raman Spectroscopy In Tissuesmentioning

confidence: 99%

The Clinical Application of Raman Spectroscopy for Breast Cancer Detection

Gao

Han

et al. 2017

Journal of Spectroscopy

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“…SPIOs on the other hand have been cleared for clinical use and their lymphatic metastasis detection potential has been verified by several clinical MRI studies [7,8] making them potentially valuable for intra-operative lymph node staging. Based on this, intra-operative staging of lymph nodes as researched by several other optical techniques like Optical Coherence Tomography (OCT) [32] and Raman spectroscopy [33], could possibly also be performed using the combination of photoacoustic imaging and SPIO nanoparticles. This would mean that freshly resected nodes could be scanned during the operation, offering direct diagnostic information to the surgical team.…”

Section: Introductionmentioning

confidence: 99%

Intra‐operative ex vivo photoacoustic nodal staging in a rat model using a clinical superparamagnetic iron oxide nanoparticle dispersion

Grootendorst

Fratila

Visscher

et al. 2013

Journal of Biophotonics

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The ability to accurately detect tumor metastases in lymph nodes is essential for intra-operative staging of various malignancies. Histopathological assessment of nodes has the drawback of a time delay before results are available to the surgeon and a likelihood of missing metastases. Photoacoustic (PA) imaging has been shown to possess the potential to detect melanoma metastases in resected in toto lymph nodes based on intrinsic contrast. To extend application of the method to other malignancies, extrinsic contrast for lymphatic mapping is important. We investigate in a metastatic animal model whether clinically approved superparamagnetic iron oxide (SPIO) nanoparticles, applied for MRI, can help PA imaging for staging in an intra-operative ex vivo setting. Imaging results are compared with 14 Tesla MR images and histology. We observe that irregularities in SPIO distribution in PA images of the nodes and a decrease in contrast correlate with metastatic involvement as seen in MR images and histology. The results show that a PA based imaging technique may be valuable for nodal staging in the field of surgical oncology.

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“…Sampling from a relatively large volume of the lymph node will be appropriate for diagnostic purposes [29,30].…”

Section: Discussionmentioning

confidence: 99%

“…The needle probe design will allow for some form of bulk sampling within the lymph node of interest which is ideal for studying the macro-chemistry. Lymph nodes can be highly heterogeneous, and a bulk sampling provides a mean spectrum of the lymph node with a higher probability of contribution to the signal from diseased tissue over point spectra collected from a small area [29,30].…”

Section: Introductionmentioning

confidence: 99%

Characterisation of a fibre optic Raman probe within a hypodermic needle

et al. 2015

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General rightsThis document is made available in accordance with publisher policies. Please cite only the published version using the reference above. AbstractHere we demonstrate the first use of a multifibre Raman probe that fits inside the bore of a hypodermic needle. A Raman probe utilising multiple collection fibres provides improved signal collection efficiency in biological samples compared with a previous 2-fibre design. Furthermore, probe performance (signal-to-noise ratios (SNRs)) compared favourably with those achieved in previous Raman microscope experiments able to distinguish benign, primary malignancies and secondary malignancies in lymph nodes.Experimental measurements presented in this paper give an indication of the sampling volume of the Raman needle probe in lymphoid tissues. Liquid tissue phantoms were used, containing scattering media encompassing a range of scattering properties similar to a variety of tissue types, including lymph node tissues. To validate the appropriateness of phantoms, the sampling depth of the probe was also measured in excised lymph node tissue. Greater than 50% of Raman photons collected were found to originate between the tip of the needle and a depth of 500 µm into the tissue.The needle probe presented here achieves comparable spectral quality to numerous studies previously demonstrating Raman disease discrimination. It is expected that this approach could achieve targeted subcutaneous tissue measurements and be viable for use for the in vivo Raman diagnostics of solid organs located within a few cm below the skin's surface.

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Raman spectroscopy – A potential new method for the intra-operative assessment of axillary lymph nodes

Cited by 44 publications

References 20 publications

The Clinical Application of Raman Spectroscopy for Breast Cancer Detection

The Clinical Application of Raman Spectroscopy for Breast Cancer Detection

Intra‐operative ex vivo photoacoustic nodal staging in a rat model using a clinical superparamagnetic iron oxide nanoparticle dispersion

Characterisation of a fibre optic Raman probe within a hypodermic needle

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