Raman spectroscopic study of serum albumins: an effect of proton‐ and γ‐irradiation

Abstract

Raman spectroscopy was applied to analyse structural changes in serum albumins (bovine serum albumin, BSA; human serum albumin, HSA) following proton and g-irradiation (0.5, 5 and 50 Gy). Characteristic Raman bands of both polypeptide backbone and amino acid residues were sensitive to irradiation. Significant damage of HSA/BSA was observed only at the highest dose (50 Gy). Raman spectra confirmed radiation-induced denaturation, destruction of helical structures and aggregation of serum albumins. The differences in the dose-dependent effects of proton and g-radiation on studied proteins are discussed.

Characteristic of silicon doped diamond like carbon thin films on surface properties and human serum albumin adsorption

“…The bands of free HSA were located at 1614, 1634, 1654 cm -1 and 1669 cm -1 which is related to aromatic ring vibration, random coil, α-helix and β-sheet components, respectively, whilst the bands between 1680 -1690 cm -1 region reflects the contribution of β-turn [33]. Based on the analysis data, the free HSA protein consisted of 53.3% α-helix (1653 cm -1 ),…”

Raman spectroscopic study on sodium hyaluronate: an effect of proton and γ irradiation

“…Irradiation experiments with model biomolecules and tissues monitored by infrared (IR) and Raman spectroscopy have been used in the characterisation of possible mechanisms of radiation damage. [22 -26] In previous works [27,28] , we reported that proton and γ irradiation led to evident but dissimilar changes in the Raman markers of dsDNA and serum proteins. Observed differences reflect specific lesions of macromolecules caused by protons and γ -rays dependent on the mechanism of the radiation effect.…”

Recent advances in linear and nonlinear Raman spectroscopy II

“…Irradiation experiments using proton-and -irradiation with biomolecules such as calf thymus DNA and serum albumins have been performed by Synytsya et al 79,80 for the characterization of possible mechanisms of radiation damage. In the former system, 79 they found that these irradiations lead to dissimilar changes in the Raman markers of nucleic bases and in the sugar-phosphate backbone, whereas in serum albumin, 80 characteristic Raman bands of both polypeptide backbone and amino acid residues were sensitive to irradiation.…”

“…In the former system, 79 they found that these irradiations lead to dissimilar changes in the Raman markers of nucleic bases and in the sugar-phosphate backbone, whereas in serum albumin, 80 characteristic Raman bands of both polypeptide backbone and amino acid residues were sensitive to irradiation. The observed Raman spectra confirmed radiation-induced denaturation, destruction of helical structures and aggregation of serum albumins.…”