“…In the hydrated form, this mixture does not demonstrate phase coexistence. 8,9,28 However, we detected phase coexistence in dry films for all the studied concentrations, including this one (Fig. 4A and B).…”
Biomimetic phospholipid mixtures are actively used as models of biological membranes and materials for drug delivery in biomedical tasks. One of the essential properties of membranes formed from complex phospholipid...
“…In the hydrated form, this mixture does not demonstrate phase coexistence. 8,9,28 However, we detected phase coexistence in dry films for all the studied concentrations, including this one (Fig. 4A and B).…”
Biomimetic phospholipid mixtures are actively used as models of biological membranes and materials for drug delivery in biomedical tasks. One of the essential properties of membranes formed from complex phospholipid...
“…Figure 4 shows these parameters for the BS geometry versus the DPPC molar concentration. The region of the expected phase coexistence 49 is depicted with the dashed lines in these figures.Figure 4.Parameters of the Brillouin peak in the BS geometry for the DPPC–DOPC mixtures vs. the DPPC mole fraction. (a) The frequency of the peak maximum.…”
Section: Resultsmentioning
confidence: 99%
“…Figure 4 shows these parameters for the BS geometry versus the DPPC molar concentration. The region of the expected phase coexistence 49 is depicted with the dashed lines in these figures.…”
Section: Dppc-dopc Mixturesmentioning
confidence: 99%
“…The mixtures of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-palmitoyl-sn-glycero-3-phosphocholine (DPPC) have a composition range, where the coexistence of the So and Ld domains is well documented. 49 The mixtures of DPPC and cholesterol (Chol) are often described with the coexistence of the So and Lo domains. At the same time, there are some controversies in this description, and very recent studies, 50,51 which exploited the properties of THz acoustic waves, concluded about the bilayer homogeneity on a scale above several nanometers.…”
Multicomponent lipid bilayers are used as models for searching the origin of spatial heterogeneities in biomembranes called lipid rafts, implying the coexistence of domains of different phases and compositions within the lipid bilayer. The spatial organization of multicomponent lipid bilayers on a scale of a hundred nanometers remains unknown. Brillouin spectroscopy providing information about the acoustic phonons with the wavelength of several hundred nanometers has an unexplored potential for this problem. Here, we applied Brillouin spectroscopy for three binary bilayers composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-palmitoyl-sn-glycero-3-phosphocholine (DPPC), and cholesterol. The Brillouin experiment for the oriented planar multibilayers was realized for two scattering geometries involving phonons for the lateral and normal directions of the propagation. The DPPC-DOPC mixtures known for the coexistence of the solid-ordered and liquid-disordered phases had bimodal Brillouin peaks, revealing the phase domains with sizes more than a hundred nanometers. Analysis of the Brillouin data for the binary mixtures concluded that the lateral phonons are preferable for testing the lateral homogeneity of the bilayers, while the phonons spreading across the bilayers are sensitive to the layered packing at the mesoscopic scale.
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