Raman spectroscopic study of calf thymus DNA: an effect of proton‐ and γ‐irradiation

Abstract

Raman spectroscopy was applied to analyse structural changes in calf thymus double-stranded deoxyribonucleic acid (dsDNA) after proton-and g-irradiation (0.5, 5 and 50 Gy). Characteristic Raman bands of phosphodiester linkages, nucleic bases and deoxyribose moieties were sensitive to irradiation. A significant damage of the macromolecules was observed only at the highest dose (50 Gy) of both types of radiations. Spectral changes confirmed a radiation-induced alteration of the native structure of dsDNA. Nucleic bases, especially pyrimidines, were the most sensitive to radiation, while some alterations in the sugar-phosphate backbone were also detected. The differences in the dose-dependent effects of proton vs g-irradiation on studied biomolecule are discussed.

Raman spectroscopic study on sodium hyaluronate: an effect of proton and γ irradiation

“…Irradiation experiments with model biomolecules and tissues monitored by infrared (IR) and Raman spectroscopy have been used in the characterisation of possible mechanisms of radiation damage. [22 -26] In previous works [27,28] , we reported that proton and γ irradiation led to evident but dissimilar changes in the Raman markers of dsDNA and serum proteins. Observed differences reflect specific lesions of macromolecules caused by protons and γ -rays dependent on the mechanism of the radiation effect.…”

The changing biochemical composition and organisation of the murine oocyte and early embryo as revealed by Raman spectroscopic mapping

“…The mean spectra derived for the light blue cluster are presented in Fig. 6 and, while contributions arising from proteins and lipids are evident in all, prominent bands around 1370 and 1410 cm −1 , which are known to arise in nucleic acid from pyrimidine ring breathing and CH 2 scissoring in the backbone, respectively, [11] are also apparent in the spectrum of the immature oocyte.…”

Specific Applications of Vibrational Spectroscopy in Biomedical Engineering

“…Collected in Table 5 are the main Raman lines observed during the melting process and the B→A (Z) transitions. This technique also enables examination of the structural changes of DNA caused by radiation (Synytsya et al, 2007), temperature and pH change (Duguid et al, 1996;Erfurth & Peticolas, 1975;O'Connor et al, 1982) and the addition of metal ions or organic compounds (Duguid et al, 1993;Langlais et al, 1990;Martin et al, 1978;Martin et al, 1982). The Raman spectrum can be divided into characteristic spectral regions.…”