“…Surface-enhanced Raman scattering spectra from bacterial and eukaryotic cells in cell cultures add significantly to our understanding of the interaction of plasmonic nanostructures with the intracellular and extracellular environments. Therefore, by controlling or defining the interaction of the SERS substrate with the biological system, intrinsic SERS signals can be used to detect specific biomarkers or physiologically relevant biomolecular species inside or near individual cells, such as adenosine monophosphate (AMP), , important metabolites in normal and tumor cells, neurotransmitters, , or trehalose . Surface-enhanced Raman scattering microscopy always indicates the presence of an interaction of the substrate with the samples and enables mapping the position of plasmonic NPs. − An important aspect in SERS experiments of live cells, and when gold or silver NPs are used as plasmonic substrates rather than when cells are attached to solid sensors , or nanoelectrodes, or when substrates are inserted into cells in needle-type approaches, ,− is the control of the plasmonic properties, which are critical for SERS enhancement.…”