Raman spectroscopic analysis and imaging in two cases of benign cementoma: Comparison with dental and bone tissues

Chappard, Daniel; Guillaume, Bernard; Teman, G.; Kün‐Darbois, Jean‐Daniel

doi:10.1002/jrs.5880

Cited by 7 publications

(3 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Statistical significance of these vibrational bands indicates a certain alteration in the organic matrix so we calculated and compared intensity ratios (PO 4 (960 cm −1 )/amid I (1665 cm −1 ) and PO 4 (960 cm −1 )/nCH 2 (2940 cm −1 )), which are the standard used for determination of minerals to matrix ratio [ 32 , 33 ]. For each recorded Raman spectrum, we read off intensities, calculated the aforementioned ratios, and then used a Student’s t -test test.…”

Section: Resultsmentioning

confidence: 99%

Possibility of Human Gender Recognition Using Raman Spectra of Teeth

et al. 2021

View full text Add to dashboard Cite

Gender determination of the human remains can be very challenging, especially in the case of incomplete ones. Herein, we report a proof-of-concept experiment where the possibility of gender recognition using Raman spectroscopy of teeth is investigated. Raman spectra were recorded from male and female molars and premolars on two distinct sites, tooth apex and anatomical neck. Recorded spectra were sorted into suitable datasets and initially analyzed with principal component analysis, which showed a distinction between spectra of male and female teeth. Then, reduced datasets with scores of the first 20 principal components were formed and two classification algorithms, support vector machine and artificial neural networks, were applied to form classification models for gender recognition. The obtained results showed that gender recognition with Raman spectra of teeth is possible but strongly depends both on the tooth type and spectrum recording site. The difference in classification accuracy between different tooth types and recording sites are discussed in terms of the molecular structure difference caused by the influence of masticatory loading or gender-dependent life events.

show abstract

Section: Resultsmentioning

confidence: 99%

Possibility of Human Gender Recognition Using Raman Spectra of Teeth

et al. 2021

View full text Add to dashboard Cite

show abstract

“…[26,38] Raman spectroscopy and imaging also allow evaluation of the hydration of the different area of the graft as MMA infiltrates microporosity and substitutes to water in hydrated tissues during histological embedding. [17,29,46] The most typical peaks is at 813 cm −1 (C O C) and does not overlap with peaks of bone or β-TCP. Hydration, defined at the ratio of 813 cm −1 /ν1 phosphate band, reflects the original water content of the tissue as, in spectroscopy, the ratio of two peak intensities is proportional to the relative amounts of the two corresponding species.…”

Section: Discussionmentioning

confidence: 99%

Bone grafted with β‐TCP granules in the rabbit: A microcomputed tomographic, histologic, Raman microspectrometric, and Raman imaging study

Aguado

Goyenvalle

Guintard

et al. 2020

J Raman Spectroscopy

Self Cite

View full text Add to dashboard Cite

Bone grafting with synthetic substitutes is now the preferred technique in maxillofacial surgery. The biocompatibility of biomaterials such as betatricalcium phosphate (β-TCP) necessitates animal experimentation. Here, six rabbits received β-TCP granules in a hole drilled in the femoral condyle and were compared with ungrafted holes with a similar critical size defect on the control-lateral side. Microcomputed tomography confirmed that new bone trabeculae had invaded the grafted zone after 28 days, whereas only minimal remodeling had occurred at the margins of the hole in control rabbits. Histological sections, after polymethylmethacrylate (pMMA) embedding, evidenced a direct apposition of bone onto the granules. In some areas, osteoid tissue remained unmineralized, sandwiched between the biomaterial and fully calcified bone. Raman analysis was used to characterize the differences between the calcium/phosphate biomaterial and the bone matrix containing hydroxyapatite. Luminescence of β-TCP was evidenced with a 785-nm laser used for biological analysis but not with a 532-nm laser. The ν1 phosphate allowed a clear differentiation of bone and the biomaterial as the subpeaks were not similar (960 cm −1 for bone, 949 and 970 cm −1 for β-TCP). Hydration (derived from identification of pMMA at 813 cm −1) and crystallinity could be determined. Raman imaging allowed the precise localization of these different peaks or ratios; luminescence peaks of β-TCP also allowed a clear differentiation of the material from bone.

show abstract

“…Raman spectroscopy can distinguish between different mineralised biological tissues, including enamel, dentine, cementum, and bone, and reveal vital information about the biological processes underpinning their formation and assembly 9 . Certain aspects of mineral composition, e.g., carbonate ion (CO 3 2− ) incorporation, provide insights into the pathways of bioapatite formation 10 .…”

Section: Introductionmentioning

confidence: 99%

High-resolution Raman spectroscopy reveals compositional differences between pigmented incisor enamel and unpigmented molar enamel in Rattus norvegicus

Shah

2023

Sci Rep

View full text Add to dashboard Cite

Dental enamel is a peculiar biological tissue devoid of any self-renewal capacity as opposed to bone. Thus, a thorough understanding of enamel composition is essential to develop novel strategies for dental enamel repair. While the mineral found in bone and dental enamel is generally viewed as the biologically-produced equivalent of hydroxy(l)apatite, the formation of these bioapatites is controlled by different organic matrix frameworks—mainly type-I collagen in bone and amelogenin in enamel. In lower vertebrates, such as rodents, two distinct types of enamel are produced. Iron-containing pigmented enamel protects the continuously growing incisor teeth while magnesium-rich unpigmented enamel covers the molar teeth. Using high-resolution Raman spectroscopy, scanning electron microscopy, and energy dispersive X-ray spectroscopy, this work explores the differences in acid phosphate (HPO42−), carbonate (CO32−), hydroxyl (OH−), iron, and magnesium content of pigmented incisor enamel and unpigmented molar enamel of Sprague Dawley rats. Bundles of hydroxy(l)apatite nanowires comprise the enamel prisms, where prisms in pigmented enamel are wider and longer than those in unpigmented molars. In contrast to magnesium-rich unpigmented enamel, higher mineral crystallinity, and higher HPO42− and OH− levels are hallmark features of iron-rich pigmented enamel. Furthermore, the apparent absence of iron oxides or oxy(hydroxides) indicates that iron is introduced into the apatite lattice at the expense of calcium, albeit in amounts that do not alter the Raman signatures of the PO43− internal modes. Compositional idiosyncrasies of iron-rich pigmented and nominally iron-free unpigmented enamel offer new insights into enamel biomineralisation supporting the notion that, in rodents, ameloblast function differs significantly between the incisors and the molars.

show abstract

Raman spectroscopic analysis and imaging in two cases of benign cementoma: Comparison with dental and bone tissues

Cited by 7 publications

References 29 publications

Possibility of Human Gender Recognition Using Raman Spectra of Teeth

Possibility of Human Gender Recognition Using Raman Spectra of Teeth

Bone grafted with β‐TCP granules in the rabbit: A microcomputed tomographic, histologic, Raman microspectrometric, and Raman imaging study

High-resolution Raman spectroscopy reveals compositional differences between pigmented incisor enamel and unpigmented molar enamel in Rattus norvegicus

Contact Info

Product

Resources

About