Raman scattering of collagen, gelatin, and elastin

Frushour, Bruce G.; Koenig, Jack L.

doi:10.1002/bip.1975.360140211

Cited by 381 publications

(332 citation statements)

References 20 publications

(2 reference statements)

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“…Spectra from the internal surfaces of both the rock pigeon and chicken eggshells provided evidence of calcite and a matrix protein (e.g. collagen or albumin; Frushour and Koenig, 1975). Five spectral peaks attributed to protein were well resolved in analyses of the chicken eggshell (internal), and 17 protein-attributed peaks could be identified in spectra from the rock pigeon eggshell (internal).…”

Section: Identifying Other Eggshell Constituentsmentioning

confidence: 99%

Analysing avian eggshell pigments with Raman spectroscopy

Thomas

Hauber

Hanley

et al. 2015

Journal of Experimental Biology

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Avian eggshells are variable in appearance, including coloration. Here, we demonstrate that Raman spectroscopy can provide accurate diagnostic information about major eggshell constituents, including the pigments biliverdin and protoporphyrin IX. Eggshells pigmented with biliverdin showed a series of pigment-diagnostic Raman peaks under 785 nm excitation. Eggshells pigmented with protoporphyrin IX showed strong emission under 1064 nm and 785 nm excitation, whereas resonance Raman spectra (351 nm excitation) showed a set of protoporphyrin IX informative peaks characteristic of protoporphyrin IX. As representative examples, we identified biliverdin in the olive green eggshells of elegant crested tinamous (Eudromia elegans) and in the blue eggshells of extinct upland moa (Megalapteryx didinus). This study encourages the wider use of Raman spectroscopy in pigment and coloration research and highlights the value of this technique for non-destructive analyses of museum eggshell specimens.

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Section: Identifying Other Eggshell Constituentsmentioning

confidence: 99%

Analysing avian eggshell pigments with Raman spectroscopy

Thomas

Hauber

Hanley

et al. 2015

Journal of Experimental Biology

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“…Table 1 summarizes the Raman shift and the assignment of the bands, which are discussed in terms of riboflavin-UVA and collagen interactions. [26][27][28][29][30][31] Fig. 1 Raman spectra of the normal and cross-linked human sclera tissue in three spectral ranges of (a) 200 to 3200 cm − 1 , (b) 200 to 1800 cm − 1 , and (c) 2800 to 3200 cm − 1 , respectively.…”

Section: Histological Analysismentioning

confidence: 99%

Effect of cross-linking with riboflavin and ultraviolet A on the chemical bonds and ultrastructure of human sclera

et al. 2011

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Abstract. This study examined the effect of the cross-linking with riboflavin-ultraviolet A (UVA) irradiation on the chemical bonds and ultrastructural changes of human sclera tissues using Raman spectroscopy and atomic force microscopy (AFM). Raman spectroscopy of the normal and cross-linked human sclera tissue revealed different types of the riboflavin-UVA and collagen interactions, which could be identified from their unique peaks, intensity, and shape. Raman spectroscopy can prove to be a powerful tool for examining the chemical bond of collagenous tissues at the molecular level. After riboflavin-UVA treatment, unlike a regular parallel arrangement of normal collagen fibrils, the AFM image revealed interlocking arrangements of collagen fibrils. The observed changes in the surface topography of the collagen fibrils, as well as in their chemical bonds in the sclera tissue, support the formation of interfibrilar cross-links in sclera tissues. C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).

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“…Thus, it can provide critical data on triple helix to random coil transitions that may occur in dentin collagen as a result of heat, acid or other degrading reagents. In the Raman spectra of collagen, bands associated with amide I and III are particularly sensitive to conformational changes in the triple helix [73].…”

Section: Dentin Smear Layermentioning

confidence: 99%

Multiscale mechanics of hierarchical structure/property relationships in calcified tissues and tissue/material interfaces

Katz

Misra

Spencer

et al. 2007

Materials Science and Engineering: C

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This paper presents a review plus new data that describes the role hierarchical nanostructural properties play in developing an understanding of the effect of scale on the material properties (chemical, elastic and electrical) of calcified tissues as well as the interfaces that form between such tissues and biomaterials. Both nanostructural and microstructural properties will be considered starting with the size and shape of the apatitic mineralites in both young and mature bovine bone. Microstructural properties for human dentin and cortical and trabecular bone will be considered. These separate sets of data will be combined mathematically to advance the effects of scale on the modeling of these tissues and the tissue/biomaterial interfaces as hierarchical material/structural composites. Interfacial structure and properties to be considered in greatest detail will be that of the dentin/adhesive (d/a) interface, which presents a clear example of examining all three material properties, (chemical, elastic and electrical). In this case, finite element modeling (FEA) was based on the actual measured values of the structure and elastic properties of the materials comprising the d/a interface; this combination provides insight into factors and mechanisms that contribute to premature failure of dental composite fillings. At present, there are more elastic property data obtained by microstructural measurements, especially high frequency ultrasonic wave propagation (UWP) and scanning acoustic microscopy (SAM) techniques. However, atomic force microscopy (AFM) and nanoindentation (NI) of cortical and trabecular bone and the dentin-enamel junction (DEJ) among others have become available allowing correlation of the nanostructural level measurements with those made on the microstructural level.

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Raman scattering of collagen, gelatin, and elastin

Cited by 381 publications

References 20 publications

Analysing avian eggshell pigments with Raman spectroscopy

Analysing avian eggshell pigments with Raman spectroscopy

Effect of cross-linking with riboflavin and ultraviolet A on the chemical bonds and ultrastructure of human sclera

Multiscale mechanics of hierarchical structure/property relationships in calcified tissues and tissue/material interfaces

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