Raman microspectroscopy of intracellular cholesterol crystals in cultured bovine coronary artery endothelial cells

Hawi, Sharon R.; Nithipatikom, Kasem; Wohlfeil, Eric R.; Adar, Fran; Campbell, William B.

doi:10.1016/s0022-2275(20)37177-7

Cited by 32 publications

(8 citation statements)

References 21 publications

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“…The molecular information obtained from these type of studies can be important in understanding the pathogenesis of atherosclerosis. The importance of Raman microspectroscopy was shown by Hawi et al [39] who demonstrated that it was possible to characterize the exact chemical composition of intracellular crystals that were formed in cultured bovine endothelial cells in situ.…”

Section: Raman Spectroscopy Of Artery Tissuementioning

confidence: 99%

Raman Spectroscopy of Atherosclerosis

Poll

Römer

Puppels

et al. 2002

European Journal of Cardiovascular Prevention & Rehabilitat

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Raman spectroscopy can provide detailed, quantitative information about the chemical composition of an atherosclerotic plaque. Recent development of compact clinical Raman systems and specially developed Raman catheters allow the remote, intravascular application of the technique. This shows prospects for the detection of coronary plaques that are prone to rupture and for the quantitative study of atherosclerosis in terms of chemical composition. This review discusses the basic principles and advances of Raman spectroscopy in the field of atherosclerosis, and its future directions in cardiovascular medicine.

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Section: Raman Spectroscopy Of Artery Tissuementioning

confidence: 99%

Raman Spectroscopy of Atherosclerosis

Poll

Römer

Puppels

et al. 2002

European Journal of Cardiovascular Prevention & Rehabilitat

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“…Fewer publications have dealt with spectroscopy of the lipid molecules themselves; several representative articles are mentioned here. Raman microspectroscopy has been used for in situ characterization of cholesterol crystals in artery endothelial cells (635). The structure of lipid-cholesterol vesicles as a function of pressure has been investigated using Raman frequencies, band shapes, and splittings to characterize conformer population, reorientational fluctuations, acyl chain interactions, and phase transitions in the lipid bilayers (636).…”

Section: Biological Applications Of Ramanmentioning

confidence: 99%

Raman Spectroscopy

et al. 1998

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“…Raman spectroscopy has been used as a research tool for analysis of synovial aspirates for MSU, 13 CPPD 14 and cholesterol crystals. 15 In these studies, crystals had to be visually identied and spectral data were collected from individual crystals using high magnication objectives. Previous work from our lab demonstrated that Raman spectroscopy can identify crystals concentrated from synovial aspirates at clinically relevant concentrations, 16 and showed that the diagnostic performance of Raman analysis compared favorably over polarized microscopic diagnosis in a limited number of clinical samples.…”

Section: Introductionmentioning

confidence: 99%

A customized Raman system for point-of-care detection of arthropathic crystals in the synovial fluid

Yang

Akkuş

2014

Analyst

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Monosodium urate (MSU) and calcium pyrophosphate dihydrate (CPPD) are the most frequently observed crystals in joint space, leading to painful arthropathies. Correct diagnosis of the crystal identity is critical for the appropriate course of treatment. In this work, a custom Raman device in combination with a practical and efficient sample preparation method is used for chemically selective diagnosis of MSU and CPPD crystals in an automated fashion. The samples were prepared by a brief enzymatic digestion treatment of synovial fluid followed by a customized filtration process which was able to congregate crystals over a submillimeter sized spot. The data acquisition and collection was automated to collect multiple spectra distributed over the filtration spot. The performance of the cost-efficient Raman system was compared to a research-grade high fidelity Raman instrument. The custom-designed Raman device could detect MSU crystals at sub-clinical concentration of 0.1 μg/mL, and 1 μg/mL for CPPD crystals. This practical sample preparation approach in tandem with the low-cost customized Raman device has a potential to be a novel tool for point-and-shoot Raman diagnosis of arthritic crystals in synovial fluid at the point of care.

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Raman microspectroscopy of intracellular cholesterol crystals in cultured bovine coronary artery endothelial cells

Cited by 32 publications

References 21 publications

Raman Spectroscopy of Atherosclerosis

Raman Spectroscopy of Atherosclerosis

Raman Spectroscopy

A customized Raman system for point-of-care detection of arthropathic crystals in the synovial fluid

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