Raman microspectroscopy for the development and screening of recombinant cell lines

Brauchle, Eva; Schenke‐Layland, Katja

doi:10.1002/biot.201600412

Cited by 11 publications

(10 citation statements)

References 9 publications

(24 reference statements)

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“…PCA was employed as an unsupervised multivariate approach to analyse data and the effects of ACT in each cell localisation by identification and differentiation of different spectral groups using scatter plots and loadings showing a representation of spectral features responsible for the variance between data groups according to wavenumbers. The order of the PCs denotes their importance to the data set and PC1 describes the highest amount of variation .…”

Section: Methodsmentioning

confidence: 99%

An in vitro study of the interaction of the chemotherapeutic drug Actinomycin D with lung cancer cell lines using Raman micro‐spectroscopy

Farhane¹,

Bonnier

Byrne³

2017

Journal of Biophotonics

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The applications of Raman microspectroscopy have been extended in recent years into the field of clinical medicine, and specifically in cancer research, as a non-invasive diagnostic method in vivo and ex vivo, and the field of pharmaceutical development as a label-free predictive technique for new drug mechanisms of action in vitro. To further illustrate its potential for such applications, it is important to establish its capability to fingerprint drug mechanisms of action and different cellular reactions. In this study, cytotoxicity assays were employed to establish the toxicity profiles for 48 and 72 hours exposure of lung cancer cell lines, A549 and Calu-1, after exposure to Actinomycin D (ACT) and Raman micro-spectroscopy was used to track its mechanism of action at subcellular level and subsequent cellular responses. Multivariate data analysis was used to elucidate the spectroscopic signatures associated with ACT chemical binding and cellular resistances. Results show that the ACT uptake and mechanism of action are similar in the 2 cell lines, while A549 cells exhibits spectral signatures of resistance to apoptosis related to its higher chemoresistance to the anticancer drug ACT. The observations are discussed in comparison to previous studies of the similar anthracyclic chemotherapeutic agent Doxorubicin. A, Preprocessed Raman spectrum of ACT stock solution dissolved in sterile water and mean spectrum with SD of (B) nucleolus, (C) nucleus and (D) cytoplasm of A549 cell lines after 48 hours exposure to the corresponding IC 50 . K E Y W O R D SA549, Actinomycin D, binding signature, Calu-1, Raman micro-spectroscopy | INTRODUCTIONRaman micro-spectroscopy is a non-invasive analytical tool, whose potential in clinical applications to distinguish between normal and cancer cells and monitoring drug mechanisms of action has already been demonstrated [1][2][3][4][5]. Therefore, it can potentially be used in preclinical development for the prediction of chemotherapeutic efficacy and potentially ultimately as a companion diagnostic tool. In order to assure its application as a predictive tool, Raman spectroscopy should provide information about drug mechanisms of action and cellular reactions or resistance. Previous studies were made using Raman microspectroscopy and chemotherapeutic drugs such as Cisplatin [6], Vincristine [6], Erlotinib [7], Panitumumab [8] and Doxorubicin (DOX) [9] showing the potential of this

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Section: Methodsmentioning

confidence: 99%

An in vitro study of the interaction of the chemotherapeutic drug Actinomycin D with lung cancer cell lines using Raman micro‐spectroscopy

Farhane¹,

Bonnier

Byrne³

2017

Journal of Biophotonics

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“…Raman spectroscopy (RS) relies on inelastic scattering of light, a unique technique capable of label‐free and non‐destructive probing of cellular molecules, for example to determine highly specific diagnostic molecular fingerprints . RS has been widely used for clinical diagnosis of different types of cancers, such as oral, skin, breast, gastro intestinal tract, cervix, bladder, prostate and lung .…”

Section: Introductionmentioning

confidence: 99%

A comparative evaluation of diffuse reflectance and Raman spectroscopy in the detection of cervical cancer

Shaikh

Prabitha

Dora

et al. 2016

Journal of Biophotonics

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Optical spectroscopic techniques show improved diagnostic accuracy for non-invasive detection of cervical cancers. In this study, sensitivity and specificity of two in vivo modalities, i.e diffuse reflectance spectroscopy (DRS) and Raman spectroscopy (RS), were compared by utilizing spectra recorded from the same sites (67 tumor (T), 22 normal cervix (C), and 57 normal vagina (V)). Data was analysed using principal component - linear discriminant analysis (PC-LDA), and validated using leave-one-out-cross-validation (LOOCV). Sensitivity, specificity, positive predictive value and negative predictive value for classification between normal (N) and tumor (T) sites were 91%, 96%, 95% and 93%, respectively for RS and 85%, 95%, 93% and 88%, respectively for DRS. Even though DRS revealed slightly lower diagnostic accuracies, owing to its lower cost and portability, it was found to be more suited for cervical cancer screening in low resource settings. On the other hand, RS based devices could be ideal for screening patients with centralised facilities in developing countries.

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“…The presence of eumelanins and pheomelanins in human skin offer a potentially useful clinical method for noninvasively investigating the eyes [ 310,311 ] or the skin. [ 312,313 ] Indeed, the use of Raman spectroscopy to identify melanin within cells or tissues [ 314–317 ] is becoming more routine in biomedical fields as it is emerging that differences between normal and cancer cells can be detected [ 318–321 ] and as a tool to predict cancer cells response to various therapies [ 318 ] potentially allowing patients to receive specific treatments more likely to work for them as current treatment regimes are standardized with patients following on from one failed therapy to another based on the therapies generalized success rate; thereby highlighting the importance of fundamental analytical studies of melanins for red biotechnologies.…”

Section: Analysis Of Melaninsmentioning

confidence: 99%

Melanins as Sustainable Resources for Advanced Biotechnological Applications

et al. 2020

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Melanins are a class of biopolymers that are widespread in nature and have diverse origins, chemical compositions, and functions. Their chemical, electrical, optical, and paramagnetic properties offer opportunities for applications in materials science, particularly for medical and technical uses. This review focuses on the application of analytical techniques to study melanins in multidisciplinary contexts with a view to their use as sustainable resources for advanced biotechnological applications, and how these may facilitate the achievement of the United Nations Sustainable Development Goals.

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Raman microspectroscopy for the development and screening of recombinant cell lines

Cited by 11 publications

References 9 publications

An in vitro study of the interaction of the chemotherapeutic drug Actinomycin D with lung cancer cell lines using Raman micro‐spectroscopy

An in vitro study of the interaction of the chemotherapeutic drug Actinomycin D with lung cancer cell lines using Raman micro‐spectroscopy

A comparative evaluation of diffuse reflectance and Raman spectroscopy in the detection of cervical cancer

Melanins as Sustainable Resources for Advanced Biotechnological Applications

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