Raman micro-spectroscopy reveals the spatial distribution of fumarate in living cells (Conference Presentation)

Kamp, Marlous; Surmacki, Jakub; Zecchini, Vincent; Chrabąszcz, Karolina; Mondejar, Marc Segarra; Young, Tim; Frezza, Christian; Bohndiek, Sarah E.

doi:10.1117/12.2648523

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Not only is CRM useful in the identification of cell types based on their inherent characteristics but it also proves useful in differentiating between “normal” and metabolite-deficient cells. For example, one study used CRM to resolve fumarate spatially, an intermediate metabolite in the tricarboxylic acid cycle, within living murine kidney cells in vitro and tissues ex vivo [88] . This work demonstrates the capability of CRM in distinguishing between cells with normal and deficient levels of Fumarate hydratase (Fh1), an enzyme involved in fumarate metabolism, based on fumarate concentration.…”

Section: Raman Microscopymentioning

confidence: 99%

Imaging immunometabolism in situ in live animals

Molnar,

Miskolci

2024

Immunometabolism

View full text Add to dashboard Cite

Immunometabolism is a rapidly developing field that holds great promise for diagnostic and therapeutic benefits to human diseases. The field has emerged based on seminal findings from in vitro and ex vivo studies that established the fundamental role of metabolism in immune cell effector functions. Currently, the field is acknowledging the necessity of investigating cellular metabolism within the natural context of biological processes. Examining cells in their native microenvironment is essential not only to reveal cell-intrinsic mechanisms but also to understand how cross-talk between neighboring cells regulates metabolism at the tissue level in a local niche. This necessity is driving innovation and advancement in multiple imaging-based technologies to enable analysis of dynamic intracellular metabolism at the single-cell level, with spatial and temporal resolution. In this review, we tally the currently available imaging-based technologies and explore the emerging methods of Raman and autofluorescence lifetime imaging microscopy, which hold significant potential and offer broad applications in the field of immunometabolism.

show abstract

Section: Raman Microscopymentioning

confidence: 99%