Raman Imaging of Plant Cell Walls

Mateu, Batirtze Prats; Bock, P.; Gierlinger, Notburga

doi:10.1007/978-1-0716-0621-6_15

Cited by 11 publications

(4 citation statements)

References 88 publications

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“…In addition to these, some mango components were observed in these samples, as indicated by the arrows in Figure 10. PLA‐K‐1 and PLA‐K‐2 presented approximately the same mango bands, (1719–1708 cm −1 ), related to CO stretching of tannic acid; cutin 53–55 ; (1617–1610 cm −1 ) regarding CC stretching of gallic acid, tannic acid related to kernel, 54,58 phenylalanine, ferulic acid related to integument 48,55 (479–476 cm −1 ), related to CCC bending; CO torsion of alanine (τ(NH3)); starch 48,52 . PLA‐I‐1 and PLA‐I‐2 samples presented only the band at 1614–1602 cm −1 related to mango.…”

Section: Resultsmentioning

confidence: 89%

Influence of the microstructure in the biodegradability process of eco‐friendly materials based on polylactic acid and mango seed for food packaging to minimize microplastic generation

Lima,

Middea,

Reis

et al. 2024

J of Applied Polymer Sci

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In this research, mango parts (kernel and integument) were loaded into a polylactic acid (PLA) matrix by a casting process to develop biocomposite materials. These materials should present improved mechanical and thermal properties and have the potential to be used as environmentally friendly, biodegradable food packaging material with extended shelf life. For this purpose, the research was divided into two parts: step 1, ground materials of a certain particle size from a previous work1 and step 2, which are the materials from Step 1 ground again. The samples were characterized by x‐ray diffraction, scanning electron microscopy (SEM), laser granulometry, texture tests, and thermal analysis. The difference between them was particle size distribution, impacting the materials' mechanical strength and degradation rate. Three biocomposites were made (PLA/kernel, PLA/integument, and PLA/kernel/integument) in both steps. SEM was used to observe the size distribution of the mango seed and sample morphology. In addition, resistance values increased with the addition of kernel or integument particles. Biocomposite biodegradability was also evaluated. Based on the results of this study, the biocomposites showed high mechanical properties and thermal stability, making them suitable for applications such as food packaging and structural components to help reduce environmental loads and microplastic generation.

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Section: Resultsmentioning

confidence: 89%

Influence of the microstructure in the biodegradability process of eco‐friendly materials based on polylactic acid and mango seed for food packaging to minimize microplastic generation

Lima,

Middea,

Reis

et al. 2024

J of Applied Polymer Sci

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“…These last three peaks were related to lignin. The 1,600 cm –1 was assigned to aromatic ring mode, the 1,630 cm –1 to ring conjugated C=C stretching of coniferaldehyde, and the 1,660 cm –1 to ring conjugated C=C stretching of coniferyl alcohol ( Hänninen et al, 2011 ; Mateu et al, 2020 ). It is noteworthy that these three peaks appeared in all phenolic compounds.…”

Section: Resultsmentioning

confidence: 99%

“…Researchers discovered that phenolic compounds show defense responses characterized by rapid and early accumulation at the infection site, which prevents the pathogen from spreading ( Chérif et al, 1991 ). The formation of lignin was one of the responses to these conditions ( Zhao et al, 2019 ; Mateu et al, 2020 ; Saletnik et al, 2021 ). In bundle sheath cells, cellulose was distributed similarly to lignin.…”

Section: Discussionmentioning

confidence: 99%

“…Raman microspectroscopy technique has shown a great deal of promise in finding out compositional, structural and spatial information about cellular polymers, due to its high spatial resolution and spectral fingerprint response characteristics ( Zhao et al, 2019 ; Mateu et al, 2020 ; Saletnik et al, 2021 ). A remarkable perspective on visualizing cellular walls can be gained by Raman microspectroscopy, which provides detailed information about the physical properties and chemical composition of the cell wall in plants ( Pohling et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

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A Spatial-Temporal Analysis of Cellular Biopolymers on Leaf Blight-Infected Tea Plants Using Confocal Raman Microspectroscopy

Sanaeifar

et al. 2022

Front. Plant Sci.

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The objective of the present study was to characterize the temporal and spatial variation of biopolymers in cells infected by the tea leaf blight using confocal Raman microspectroscopy. We investigated the biopolymers on serial sections of the infection part, and four sections corresponding to different stages of infection were obtained for analysis. Raman spectra extracted from four selected regions (circumscribing the vascular bundle) were analyzed in detail to enable a semi-quantitative comparison of biopolymers on a micron-scale. As the infection progressed, lignin and other phenolic compounds decreased in the vascular bundle, while they increased in both the walls of the bundle sheath cells as well as their intracellular components. The amount of cellulose and other polysaccharides increased in all parts as the infection developed. The variations in the content of lignin and cellulose in different tissues of an individual plant may be part of the reason for the plant’s disease resistance. Through wavelet-based data mining, two-dimensional chemical images of lignin, cellulose and all biopolymers were quantified by integrating the characteristic spectral bands ranging from 1,589 to 1,607 cm–1, 1,087 to 1,100 cm–1, and 2,980 to 2,995 cm–1, respectively. The chemical images were consistent with the results of the semi-quantitative analysis, which indicated that the distribution of lignin in vascular bundle became irregular in sections with severe infection, and a substantial quantity of lignin was detected in the cell wall and inside the bundle sheath cell. In serious infected sections, cellulose was accumulated in vascular bundles and distributed within bundle sheath cells. In addition, the distribution of all biopolymers showed that there was a tylose substance produced within the vascular bundles to prevent the further development of pathogens. Therefore, confocal Raman microspectroscopy can be used as a powerful approach for investigating the temporal and spatial variation of biopolymers within cells. Through this method, we can gain knowledge about a plant’s defense mechanisms against fungal pathogens.

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Strategies for Measuring Cytosolic Sugar Concentrations in Plant Cells

Li¹,

Liesche²

2022

Annual Plant Reviews Online

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The primary products of plant photosynthesis are soluble sugars such as glucose, fructose, and sucrose. These form the basis of every cell's carbon and energy metabolism. One major limitation for the investigation of soluble sugar metabolism is the difficulty associated with measuring their concentrations in different subcellular compartments. This applies especially to the cytosol, the site of many important enzymatic reactions, as it typically only fills a thin area between the vacuole and plasma membrane. In this article, we discuss the advantages and disadvantages of methods that have been used to probe cytosolic sugar concentrations, including microautoradiography, plasmolysis, non‐aqueous fractionation, Fourier transform infrared (FTIR) microspectroscopy, Raman microspectroscopy, mass spectrometry imaging, Förster resonance energy transfer (FRET) nanosensors, direct sampling, and theoretical modelling. Perspectives regarding different use cases and future developments are provided.

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Raman Imaging of Plant Cell Walls

Cited by 11 publications

References 88 publications

Influence of the microstructure in the biodegradability process of eco‐friendly materials based on polylactic acid and mango seed for food packaging to minimize microplastic generation

Influence of the microstructure in the biodegradability process of eco‐friendly materials based on polylactic acid and mango seed for food packaging to minimize microplastic generation

A Spatial-Temporal Analysis of Cellular Biopolymers on Leaf Blight-Infected Tea Plants Using Confocal Raman Microspectroscopy

Strategies for Measuring Cytosolic Sugar Concentrations in Plant Cells

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