Raman imaging of hemozoin within the food vacuole of <i>Plasmodium falciparum</i> trophozoites

Wood, Bayden Robert; Langford, Steven J.; Cooke, Brian M.; Glenister, Fiona Kim; Lim, Janelle; McNaughton, Donald

doi:10.1016/s0014-5793(03)00975-x

Cited by 93 publications

(105 citation statements)

References 33 publications

(48 reference statements)

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“…At low laser power, visible lasers can also be used for measuring Raman spectra of viable cells, especially when the laser excites resonance Raman scattering of specific molecules (see Sections 2.4 and 4.2). Resonance Raman spectra of haemoglobin and hemozoin in malaria-infected erythrocytes have been reported using lasers with wavelength of 488 nm, 514 nm, 564 nm, 633 nm, and 780 nm (2 mW power and integration times of 10 s integration times) [179]. A 632.8 nm laser at 1 mW power with 2 min acquisition time (defocused for wide-field imaging) was also reported for studying the oxygenation process of a human erythrocyte [180].…”

Section: Laser Damagementioning

confidence: 99%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

258

189

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Raman spectroscopy is an increasingly popular technique in many areas including biology and medicine.It is based on Raman scattering, a phenomenon in which incident photons lose or gain energy via interactions with vibrating molecules in a sample. These energy shifts can be used to obtain information regarding molecular composition of the sample with very high accuracy. Applications of Raman spectroscopy in the life sciences have included quantification of biomolecules, hyperspectral molecular imaging of cells and tissue, medical diagnosis, and others. This review briefly presents the physical origin of Raman scattering explaining the key classical and quantum mechanical concepts. Variations of the Raman effect will also be considered, including resonance, coherent, and enhanced Raman scattering. We discuss the molecular origins of prominent bands often found in the Raman spectra of biological samples. Finally, we examine several variations of Raman spectroscopy techniques in practice, looking at their applications, strengths, and challenges. This review is intended to be a starting resource for scientists new to Raman spectroscopy, providing theoretical background and practical examples as the foundation for further study and exploration.

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Section: Laser Damagementioning

confidence: 99%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

258

189

View full text Add to dashboard Cite

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“…Raman spectroscopy was successfully used to compare healthy erythrocytes and erythrocytes infected with Plasmodium falciparum. 16 RMS was also used to monitor molecular changes in red blood cells during Plasmodium infection in mice, following malaria disease progression over the course of 7 days. 17 Changes in the Raman spectra of cells was associated with the loss of hemoglobin and increase of hemozoin 4 days PI.…”

Section: Raman Spectroscopy Of Live T Gondii During Infectionmentioning

confidence: 99%

“…For example, RMS has been utilized to study the protozoan pathogen Plasmodium falciparum, the agent of human malaria. 16,17 Also, we previously developed a new method of measuring spatially-resolved Raman spectra of human cells by selective-sampling Raman microscopy and employed this method for the detection of the protozoan Neospora caninum in human blood-brain-barrier endothelial cells. 18 Herein, we examined the feasibility of using RMS to analyze molecular changes during T. gondii infection at a single cell level.…”

Section: Introductionmentioning

confidence: 99%

Analysis of interaction between the apicomplexan protozoan Toxoplasma gondii and host cells using label-free Raman spectroscopy

Naemat

Elsheikha

Al-sandaqchi

et al. 2015

Analyst

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Label-free imaging using Raman micro-spectroscopy (RMS) was used to characterize the spatio-temporal molecular changes of T. gondii tachyzoites and their host cell microenvironment. Raman spectral maps were recorded from isolated T. gondii tachyzoites and T. gondii-infected human retinal cells at 6 hr, 24 hr and 48 hr postinfection. Principal component analysis (PCA) of the Raman spectra of paraformaldehyde-fixed infected cells indicated a significant increase in the amount of lipids and proteins in the T. gondii tachyzoites as the infection progresses within host cells. These results were confirmed by experiments carried out on live T. gondii-infected cells and were correlated with an increase in the concentration of proteins and lipids required for the replication of this intracellular pathogen. These findings demonstrate the potential of RMS to characterize time-and spatially-dependent molecular interactions between intracellular pathogens and the host cells. Such information may be useful for discovery of pharmacological targets or screening compounds with potential neuroprotective activity for eminent effects of changes in brain infection control practices. 2

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“…β-hematin, can be formed in vitro from hematin under acidic conditions. With FTIR spectrophotometer, shows that the bond between the iron-carboxylate ion of the two molecules have the same hemozoin heme in the analogues, namely β-hematin [12]. β-Hematin crystal can be further measured by Elisa reader absorption at a wavelength of 405 nm.…”

Section: Synthesis Of Antiplasmodium Of 110-phenanthroline Derivativementioning

confidence: 99%

Synthesis and Heme Polymerization Inhibitory Activity (HPIA) Assay of Antiplasmodium of (1)-N-(3,4-Dimethoxybenzyl)-1,10-Phenanthrolinium Bromide from Vanillin

Fitriastuti¹,

Mardjan²,

Jumina³

et al. 2014

Indones. J. Chem.

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The synthesis of (1)-N-(3,4-dimethoxy-benzyl h to yield veratraldehyde in the form of light yellow solid (79% yield). Methylation product was reduced using sodium borohydride (NaBH 4 ) with grinding method and yielded veratryl alcohol in the form of yellow liquid (98% yield). Veratryl alcohol was brominated using PBr 3 to yield yellowish black liquid (85% yield). The final step was benzylation of 1,10-phenanthroline monohydrate with the synthesized veratryl bromide under reflux condition in acetone for 14 h to afford (1)-N-(3,4-dimethoxy-benzyl)-1,10-phenanthrolinium bromide (84%) as yellow solid with melting point of 166-177°C. The structures of products were characterized by FT-IR, GC-MS and

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Raman imaging of hemozoin within the food vacuole of Plasmodium falciparum trophozoites

Cited by 93 publications

References 33 publications

Raman spectroscopy: techniques and applications in the life sciences

Raman spectroscopy: techniques and applications in the life sciences

Analysis of interaction between the apicomplexan protozoan Toxoplasma gondii and host cells using label-free Raman spectroscopy

Synthesis and Heme Polymerization Inhibitory Activity (HPIA) Assay of Antiplasmodium of (1)-N-(3,4-Dimethoxybenzyl)-1,10-Phenanthrolinium Bromide from Vanillin

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