Raman‐controlled pyruvate feeding to control metabolic activity and product quality in continuous biomanufacturing
Patrick Romann,
Sebastian Schneider,
Daniela Tobler
et al.
Abstract:BackgroundDespite technological advances ensuring stable cell culture perfusion operation over prolonged time, reaching a cellular steady‐state metabolism remains a challenge for certain manufacturing cell lines. This study investigated the stabilization of a steady‐state perfusion process producing a bispecific antibody with drifting product quality attributes, caused by shifting metabolic activity in the cell culture.Main methodsA novel on‐demand pyruvate feeding strategy was developed, leveraging lactate as… Show more
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