RamA and RamB are global transcriptional regulators in Corynebacterium glutamicum and control genes for enzymes of the central metabolism

Auchter, Marc; Cramer, Annette; Hüser, Andrea T.; Rückert, Christian; Emer, Denise; Schwarz, Peggy; Arndt, Annette; Lange, Christian; Kalinowski, Jörn; Wendisch, Volker F.; Eikmanns, Bernhard J.

doi:10.1016/j.jbiotec.2010.07.001

Cited by 67 publications

(66 citation statements)

References 68 publications

(76 reference statements)

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“…Cell samples for total RNA preparation were taken from two independently grown C. glutamicum cultures of each strain. The isolation of total RNA was carried out as described previously (6,36). The respective RNA preparations were used in two independent DNA microarray hybridization assays by applying label swapping.…”

Section: Methodsmentioning

confidence: 99%

“…The respective RNA preparations were used in two independent DNA microarray hybridization assays by applying label swapping. The labeling of probes and DNA microarray hybridization were carried out as described previously (6,36). To minimize the number of false-positive signals, the data were stringently filtered to obtain genes with at least six statistically significant values out of eight technical replicates present on the two microarrays along with an error probability of less than 5% for the Student t test.…”

Section: Methodsmentioning

confidence: 99%

“…C. glutamicum is also able to use ethanol as a sole carbon and energy source, using alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) for NAD-dependent oxidations to acetate, which is then activated to acetyl coenzyme A (acetyl-CoA) and channeled into the citric acid and glyoxylate cycles (2,4,5,44). The ADH gene (adhA) of C. glutamicum has been shown to be subject to complex, carbon source-dependent regulation by the global transcriptional regulators RamA, RamB, and GlxR (2,6,43). In the course of further studies on the transcriptional regulation of the adhA gene, we recently identified the DeoR-type transcriptional regulator Cg0146 as the fourth regulator involved in the expression control of adhA (7).…”

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confidence: 99%

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Arabitol Metabolism of Corynebacterium glutamicum and Its Regulation by AtlR

Laslo

Zaluskowski

Gabris

et al. 2011

Journal of Bacteriology

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Expression profiling of Corynebacterium glutamicum in comparison to a derivative deficient in the transcriptional regulator AtlR (previously known as SucR or MtlR) revealed eight genes showing more than 4-fold higher mRNA levels in the mutant. Four of these genes are located in the direct vicinity of the atlR gene, i.e., xylB, rbtT, mtlD, and sixA, annotated as encoding xylulokinase, the ribitol transporter, mannitol 2-dehydrogenase, and phosphohistidine phosphatase, respectively. Transcriptional analysis indicated that atlR and the four genes are organized as atlR-xylB and rbtT-mtlD-sixA operons. Growth experiments with C. glutamicum and C. glutamicum ⌬atlR, ⌬xylB, ⌬rbtT, ⌬mtlD, and ⌬sixA derivatives with sugar alcohols revealed that (

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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Arabitol Metabolism of Corynebacterium glutamicum and Its Regulation by AtlR

Laslo

Zaluskowski

Gabris

et al. 2011

Journal of Bacteriology

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“…Intriguingly, C. glutamicum PrpR was noted to have moderate similarities to the regulator of acetate metabolism, RamB [19]. However, whereas RamB can act as both a repressor and an activator [20], PrpR acts exclusively as an activator. Nevertheless, the similarity in the amino acid sequence between RamB and PrpR hinted at a common ancestry, with each regulator apparently evolving separately to eventually control degradation of the structurally closely related but different carboxylic acids, acetate or propionate [18].…”

Section: Regulation Of Methylcitrate Cycle Gene Expression -Prprmentioning

confidence: 99%

Loving the poison: the methylcitrate cycle and bacterial pathogenesis

et al. 2018

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Propionate is an abundant catabolite in nature and represents a rich potential source of carbon for the organisms that can utilize it. However, propionate and propionate-derived catabolites are also toxic to cells, so propionate catabolism can alternatively be viewed as a detoxification mechanism. In this review, we summarize recent progress made in understanding how prokaryotes catabolize propionic acid, how these pathways are regulated and how they might be exploited to develop novel antibacterial interventions.

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“…GlxR, a cAMP-responsive regulator, and RamA, a LuxR-type regulator, were first identified as the transcriptional repressor and activator, respectively, of the aceB gene, encoding malate synthase of the glyoxylate cycle (14,38). Both regulators not only activate gapA expression (15,31) but are also involved in expression of a number of genes for various physiological functions, including carbon and nitrogen metabolism, respiration, SOS and stress responses, and cell division (15,(39)(40)(41). However, in contrast to the mechanism by which SugR switches gene expression, how GlxR and RamA switch expression of genes involved in glucose and acetate metabolism in response to a carbon source provided is not fully understood.…”

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Involvement of Regulatory Interactions among Global Regulators GlxR, SugR, and RamA in Expression of ramA in Corynebacterium glutamicum

et al. 2013

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The central carbon metabolism genes in Corynebacterium glutamicum are under the control of a transcriptional regulatory network composed of several global regulators. It is known that the promoter region of ramA, encoding one of these regulators, interacts with its gene product, RamA, as well as with the two other regulators, GlxR and SugR, in vitro and/or in vivo. Although RamA has been confirmed to repress its own expression, the roles of GlxR and SugR in ramA expression have remained unclear. In this study, we examined the effects of GlxR binding site inactivation on expression of the ramA promoter-lacZ fusion in the genetic background of single and double deletion mutants of sugR and ramA. In the wild-type background, the ramA promoter activity was reduced to undetectable levels by the introduction of mutations into the GlxR binding site but increased by sugR deletion, indicating that GlxR and SugR function as the transcriptional activator and repressor, respectively. The marked repression of ramA promoter activity by the GlxR binding site mutations was largely compensated for by deletions of sugR and/or ramA. Furthermore, ramA promoter activity in the ramA-sugR double mutant was comparable to that in the ramA mutant but was significantly higher than that in the sugR mutant. Taken together, it is likely that the level of ramA expression is dynamically balanced by GlxR-dependent activation and repression by RamA along with SugR in response to perturbation of extracellular and/or intracellular conditions. These findings add multiple regulatory loops to the transcriptional regulatory network model in C. glutamicum.

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RamA and RamB are global transcriptional regulators in Corynebacterium glutamicum and control genes for enzymes of the central metabolism

Cited by 67 publications

References 68 publications

Arabitol Metabolism of Corynebacterium glutamicum and Its Regulation by AtlR

Arabitol Metabolism of Corynebacterium glutamicum and Its Regulation by AtlR

Loving the poison: the methylcitrate cycle and bacterial pathogenesis

Involvement of Regulatory Interactions among Global Regulators GlxR, SugR, and RamA in Expression of ramA in Corynebacterium glutamicum

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