RamA, a transcriptional regulator conferring florfenicol resistance in Leclercia adecarboxylata R25

Chen, Cong; Ying, Yuanyuan; Zhou, Dingfu; Zhu, Licheng; Lü, Jing; Li, Aifang; Bao, Qiyu; Zhu, Mei

doi:10.1007/s12223-020-00816-2

Cited by 7 publications

(7 citation statements)

References 33 publications

(35 reference statements)

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“…These two genes were also present of the genom of Leclercia sp. 29361 and also described to appear in this genus by other authors 81 . Lelliottia amnigena, previously classified as Enterobacter amnigena, itself…”

Section: Discussionsupporting

confidence: 56%

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Antimicrobial resistance determinants in silage

Nagy

Tóth

Papp

et al. 2021

Preprint

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Animal products may play a role in developing and spreading antimicrobial resistance in several ways. On the one hand, residues of antibiotics not adequately used in animal farming can enter the human body via food. But resistant bacteria may also be present in animal products, which can transfer the antimicrobial resistance genes (ARG) to the bacteria in the consumer's body by horizontal gene transfer. As previous studies have shown that fermented foods have a meaningful ARG content, it is indicated that such genes may also be present in silage used as mass feed in the cattle sector. In our study, we aspired to answer what ARGs occur in silage and what mobility characteristics they have? For this purpose, we have analyzed bioinformatically 40 freely available deep sequenced silage samples from shotgun metagenome next-generation sequencing. A total of 57 ARGs occurred 616 times in the samples. More than half of these ARGs are mobile because they can be linked to integrative mobile genetic elements, prophages or plasmids. We believe that our results point to a substantial source of ARG in the food chain.

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Section: Discussionsupporting

confidence: 56%

“…Sequence variants of marA have been described in Leclercia spp. 13 , while acrB and ermB have been mentioned related to this species 80,81 . In contrast, BacA, KpnH, msbA and oqxA has not been mentioned to appear within the genom of Leclercia spp.…”

Section: Discussionmentioning

confidence: 97%

Antimicrobial resistance determinants in silage

Nagy

Tóth

Papp

et al. 2021

Preprint

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“…One possible explanation is that the modification on florfenicol with COS impaired the drug-resistant machinery. Bacteria gain the resistance to florfenicol partially by the drug efflux pump system, leading to reduced drug concentration in the bacteria cells [ 28 ]. Our previous study showed that COS modification on streptomycin suppressed the MexX-MexY drug efflux pump system in P. aeruginosa [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

Chitosan Oligosaccharides Coupling Inhibits Bacterial Biofilm-Related Antibiotic Resistance against Florfenicol

Yuan

Liu

et al. 2020

Molecules

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The formation of bacterial biofilms has increased the resistance of bacteria to various environmental factors and is tightly associated with many persistent and chronic bacterial infections. Herein we design a strategy conjugating florfenicol, an antibiotic commonly used in the treatment of streptococcus, with the antimicrobial biomaterial, chitosan oligosaccharides. The results demonstrated that the florfenicol-COS conjugate (F-COS) efficiently eradicated the mature Streptococcus hyovaginalis biofilm, apparently inhibiting drug resistance to florfenicol. A quantity of 250 μg/mL F-COS showed effective inhibitory activity against planktonic cells and biofilm of the bacteria, and a 4-fold improvement of the F-COS compared to unmodified florfenicol was observed. Furthermore, the conjugate showed a broad-spectrum activity against both Gram-positive and Gram-negative bacteria. It suggested that F-COS might have a potential for application in the treatment of biofilm-related infections.

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“…S. aureus ATCC 29213 and Escherichia coli ATCC 25922 were used as quality control strains in antimicrobial susceptibility testing experiments. E. coli strain DH5α and pUCP24 plasmid were used as a recipient and vector in cloning experiments ( Cheng et al, 2020 ), respectively.…”

Section: Methodsmentioning

confidence: 99%

In vitro antimicrobial activity and resistance mechanisms of the new generation tetracycline agents, eravacycline, omadacycline, and tigecycline against clinical Staphylococcus aureus isolates

et al. 2022

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In this study, we investigated the in vitro activity and resistance mechanisms of the new generation tetracycline agents, namely eravacycline, omadacycline, and tigecycline, against Staphylococcus aureus isolates. A total of 1,017 non-duplicate S. aureus isolates were collected and subjected to susceptibility testing against eravacycline, omadacycline, and tigecycline using the broth microdilution method. Tetracyclines-resistant (eravacycline/omadacycline/tigecycline-resistant) isolates were selected to elucidate the resistance mechanisms using polymerase chain reaction (PCR), cloning experiment, efflux pump inhibition, and quantitative real-time PCR. The results of the antibacterial susceptibility testing showed that compared with omadacycline, eravacycline and tigecycline had superior antibacterial activity against S. aureus isolates. Among 1,017 S. aureus, 41 tetracyclines-resistant isolates were identified. These resistant isolates possessed at least one tetracycline resistance gene and genetic mutation in the MepRAB efflux pump and 30S ribosome units. A frameshift mutation in mepB was detected in most tetracyclines-resistant strains (except for JP3349) compared with tetracyclines-susceptible (eravacycline/omadacycline/tigecycline-susceptible) strains. This was first shown to decrease susceptibility to omadacycline, but not to eravacycline and tigecycline. After treatment with eravacycline, omadacycline or tigecycline, overexpression of mepA, tet38, tet(K) and tet(L) was detected. Moreover, multi-locus sequence typing showed a major clonal dissemination type, ST5, and its variant ST764 were seen in most tetracyclines-resistant strains. To conclude, eravacycline and tigecycline exhibited better activity against S. aureus including tetracycline-resistant isolates than omadacycline. The resistance to these new generation tetracyclines due to an accumulation of many resistance mechanisms.

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RamA, a transcriptional regulator conferring florfenicol resistance in Leclercia adecarboxylata R25

Cited by 7 publications

References 33 publications

Antimicrobial resistance determinants in silage

Antimicrobial resistance determinants in silage

Chitosan Oligosaccharides Coupling Inhibits Bacterial Biofilm-Related Antibiotic Resistance against Florfenicol

In vitro antimicrobial activity and resistance mechanisms of the new generation tetracycline agents, eravacycline, omadacycline, and tigecycline against clinical Staphylococcus aureus isolates

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