Ralstonia solanacearum induces soluble amine-oxidase activity in Solanum torvum stem calli

Aribaud, Marcel; Jégo, Sylvaine; Wicker, Emmanuel; Fock, Isabelle

doi:10.1016/j.plaphy.2010.06.005

Cited by 4 publications

(3 citation statements)

References 43 publications

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“…When polyamines contribute to plant defense, it is often because their catabolism releases the plant defense signal H 2 O 2 (Marina et al ., ; Jiménez‐Bremont et al ., ; Gupta et al ., ). Although R. solanacearum induces putrescine catabolism in a resistant host (Aribaud et al ., ), we found that the activity of the putrescine catabolism enzyme diamine oxidase was 2.3‐fold repressed in R. solanacearum ‐infected versus healthy tomato plants. Conversely, polyamines can also reduce H 2 O 2 levels directly as antioxidants and indirectly by increasing host catalase activity (Gupta et al ., ).…”

Section: Discussionmentioning

confidence: 68%

Metabolomics of tomato xylem sap during bacterial wilt reveals Ralstonia solanacearum produces abundant putrescine, a metabolite that accelerates wilt disease

Lowe‐Power

Hendrich

Roepenack‐Lahaye

et al. 2017

Environmental Microbiology

116

133

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Ralstonia solanacearum thrives in plant xylem vessels and causes bacterial wilt disease despite the low nutrient content of xylem sap. We found that R. solanacearum manipulates its host to increase nutrients in tomato xylem sap, enabling it to grow better in sap from infected plants than in sap from healthy plants. Untargeted GC/MS metabolomics identified 22 metabolites enriched in R. solanacearum-infected sap. Eight of these could serve as sole carbon or nitrogen sources for R. solanacearum. Putrescine, a polyamine that is not a sole carbon or nitrogen source for R. solanacearum, was enriched 76-fold to 37 µM in R. solanacearum-infected sap. R. solanacearum synthesized putrescine via a SpeC ornithine decarboxylase. A ΔspeC mutant required ≥ 15 µM exogenous putrescine to grow and could not grow alone in xylem even when plants were treated with putrescine. However, co-inoculation with wildtype rescued ΔspeC growth, indicating R. solanacearum produced and exported putrescine to xylem sap. Intriguingly, treating plants with putrescine before inoculation accelerated wilt symptom development and R. solanacearum growth and systemic spread. Xylem putrescine concentration was unchanged in putrescine-treated plants, so the exogenous putrescine likely accelerated disease indirectly by affecting host physiology. These results indicate that putrescine is a pathogen-produced virulence metabolite.

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Section: Discussionmentioning

confidence: 68%

Metabolomics of tomato xylem sap during bacterial wilt reveals Ralstonia solanacearum produces abundant putrescine, a metabolite that accelerates wilt disease

Lowe‐Power

Hendrich

Roepenack‐Lahaye

et al. 2017

Environmental Microbiology

116

133

View full text Add to dashboard Cite

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“…In another study, it was found that the PP2Ac-silenced plants exhibited an accelerated hypersensitive response to effector protein from the fungal pathogen, Cladosporium fulvum and developed localized cell death [50]. The inoculation of Solanum torvum with Ralstonia solanacearum induced amine oxidase-like activity and the plants showed hypersensitive response to bacterial wilt [51]. In the present study, the target prediction of novel miRNAs, nta-mir5 and, nta-miR11 revealed that PP2A and amine oxidase-like genes are regulated by these novel miRNAs.…”

Section: Cr Responsive Novel Mirnas and Their Targetsmentioning

confidence: 99%

Genome‐wide identification of chromium stress‐responsive micro RNAs and their target genes in tobacco (Nicotiana tabacum) roots

Bukhari

Shang

Zhang

et al. 2015

Enviro Toxic and Chemistry

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Tobacco easily accumulates certain heavy metals in leaves and thus poses a potential threat to human health. To systematically dissect Cr-responsive microRNAs (miRNAs) and their targets at the global level, 4 small RNA libraries were constructed from the roots of Cr-treated (Cr) and Cr-free (control) for 2 contrasting tobacco genotypes,Yunyan2 (Cr-sensitive) and Guiyan1 (Cr-tolerant). Using high-throughput-sequencing-technology, the authors identified 53 conserved and 29 novel miRNA families. Comparative genomic analysis of 41 conserved Cr-responsive miRNA families revealed that 11 miRNA families showed up-regulation in Guiyan1 but unaltered in Yunyan2, and 17 miRNA families were up-regulated only in Yunyan2 under Cr stress. Only 1 family, miR6149, was down-regulated in Yunyan2 but remained unchanged in Guiyan1. Of the 29 novel miRNA families, 14 expressed differently in the 2 genotypes under Cr stress. Based on a high-throughput degradome sequencing homology search, potential targets were predicted for the 41 conserved and 14 novel Cr-responsive miRNA families. Clusters of Orthologous Groups functional category analysis revealed that some of these predicted target transcripts of miRNAs are responsive to biotic and abiotic stresses. Furthermore, the expression patterns of many Cr-responsive miRNAs were validated by stem-loop real-time transcription polymerase chain reaction. The results of the present study provide valuable information and a framework for understanding the function of miRNAs in Cr tolerance.

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“…bacterial, fungal wilts and root-knot nematodes) [1]. However, despite the fact that identification of the effectors of Torvum resistance would be extremely valuable for other solanaceous crops, particularly eggplant, very little studies have been conducted to date regarding Torvum resistance mechanism and have mainly dealt with its resistance to bacteria [2]. This scenario is mostly attributable to the paucity of database information on Torvum which, in turn is a consequence of the lack of high-throughput genomic tools (e.g.…”

Section: Introductionmentioning

confidence: 99%

Solanum torvum responses to the root-knot nematode Meloidogyne incognita

et al. 2013

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BackgroundSolanum torvum Sw is worldwide employed as rootstock for eggplant cultivation because of its vigour and resistance/tolerance to the most serious soil-borne diseases as bacterial, fungal wilts and root-knot nematodes. The little information on Solanum torvum (hereafter Torvum) resistance mechanisms, is mostly attributable to the lack of genomic tools (e.g. dedicated microarray) as well as to the paucity of database information limiting high-throughput expression studies in Torvum.ResultsAs a first step towards transcriptome profiling of Torvum inoculated with the nematode M. incognita, we built a Torvum 3’ transcript catalogue. One-quarter of a 454 full run resulted in 205,591 quality-filtered reads. De novo assembly yielded 24,922 contigs and 11,875 singletons. Similarity searches of the S. torvum transcript tags catalogue produced 12,344 annotations. A 30,0000 features custom combimatrix chip was then designed and microarray hybridizations were conducted for both control and 14 dpi (day post inoculation) with Meloidogyne incognita-infected roots samples resulting in 390 differentially expressed genes (DEG). We also tested the chip with samples from the phylogenetically-related nematode-susceptible eggplant species Solanum melongena. An in-silico validation strategy was developed based on assessment of sequence similarity among Torvum probes and eggplant expressed sequences available in public repositories. GO term enrichment analyses with the 390 Torvum DEG revealed enhancement of several processes as chitin catabolism and sesquiterpenoids biosynthesis, while no GO term enrichment was found with eggplant DEG.The genes identified from S. torvum catalogue, bearing high similarity to known nematode resistance genes, were further investigated in view of their potential role in the nematode resistance mechanism.ConclusionsBy combining 454 pyrosequencing and microarray technology we were able to conduct a cost-effective global transcriptome profiling in a non-model species. In addition, the development of an in silico validation strategy allowed to further extend the use of the custom chip to a related species and to assess by comparison the expression of selected genes without major concerns of artifacts. The expression profiling of S. torvum responses to nematode infection points to sesquiterpenoids and chitinases as major effectors of nematode resistance. The availability of the long sequence tags in S. torvum catalogue will allow precise identification of active nematocide/nematostatic compounds and associated enzymes posing the basis for exploitation of these resistance mechanisms in other species.

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Ralstonia solanacearum induces soluble amine-oxidase activity in Solanum torvum stem calli

Cited by 4 publications

References 43 publications

Metabolomics of tomato xylem sap during bacterial wilt reveals Ralstonia solanacearum produces abundant putrescine, a metabolite that accelerates wilt disease

Metabolomics of tomato xylem sap during bacterial wilt reveals Ralstonia solanacearum produces abundant putrescine, a metabolite that accelerates wilt disease

Genome‐wide identification of chromium stress‐responsive micro RNAs and their target genes in tobacco (Nicotiana tabacum) roots

Solanum torvum responses to the root-knot nematode Meloidogyne incognita

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