Ralstonia pickettii colonization of patients in an obstetric ward caused by a contaminated irrigation system

Yoneyama, A.; Yano, Hisako; Hitomi, Shigemi; Okuzumi, Katsuko; Suzuki, Riéko; Kimura, Shige

doi:10.1053/jhin.2000.0791

Cited by 18 publications

(10 citation statements)

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“…Still, the distribution in the size fractions is interesting, since in most positive samples, only the larger fraction was positive. One of the cultivated species targeted by the probe, Ralstonia pickettii, is occasionally found in clinical specimens (18,38) and is known to survive in aqueous solutions, both distilled water and saline (17,18). Other species from this cluster, such as Ralstonia solanacearum, are associated with plant disease (11,30).…”

Section: Discussionmentioning

confidence: 99%

Rapid Screening for Freshwater Bacterial Groups by Using Reverse Line Blot Hybridization

Zwart

Hannen

Agterveld

et al. 2003

Appl Environ Microbiol

102

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The identification of phylogenetic clusters of bacteria that are common in freshwater has provided a basis for probe design to target important freshwater groups. We present a set of 16S ribosomal RNA gene-based oligonucleotide probes specific for 15 of these freshwater clusters. The probes were applied in reverse line blot hybridization, a simple method that enables the rapid screening of PCR products from many samples against an array of probes. The optimized assay was made stringent to discriminate at approximately the singlemismatch level. This made 10 of the probes highly specific, with at least two mismatches to the closest noncluster member in the global database. Screening of PCR products from bacterioplankton of 81 diverse lakes from Belgium, The Netherlands, Denmark, Sweden, and Norway showed that the respective probes were reactive against 5 to 100% of the lake samples. Positive reactivity of six highly specific probes showed that bacteria from actinobacterial clusters ACK-M1 and Sta2-30 and from verrucomicrobial cluster CLO-14 occurred in at least 90% of the investigated lakes. Furthermore, bacteria from alpha-proteobacterial cluster LD12 (closely related to the marine SAR11 cluster), beta-proteobacterial cluster LD28 and cyanobacterial cluster Synechococcus 6b occurred in more than 70% of the lakes. Reverse line blot hybridization is a new tool in microbial ecology that will facilitate research on distribution and habitat specificity of target species at relatively low costs.The introduction of molecular methods into microbial ecology and the selection of rRNA genes as tools for bacterial classification (28,29,35,36) initiated a massive effort to describe bacterial diversity in the environment (15). Random cloning of PCR-amplified genes, the most common procedure for describing diversity, has added thousands of sequences of 16S rRNA genes to the nucleotide databases. These data, which could not have been retrieved through cultivation of bacteria (3,15), are gradually generating a better and more complete view of composition and diversity in several habitats. For instance, it is now clear that both ocean and inland waters have limited bacterial diversity. That is, the evenness in these aquatic systems is relatively low because a few groups of bacteria dominate the systems (10,12,13,23,24,39). Moreover, these dominating groups appear to be widespread over the globe. For freshwater, we recently analyzed all of the available 16S rRNA gene sequences from clone libraries and identified 34 phylogenetic groups that appeared in more than one data set from different lakes or rivers (39). The identification of relatively narrow groups such as these (91 to 99% sequence similarity) creates a starting point for exploration of the functions and ecology of bacteria and allows the design of probes for rapid screening and comparison of bacterial communities in many different freshwater ecosystems.In the present study we describe molecular probes targeting 16S rRNA genes from 15 of the freshwater clusters and explain ...

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Section: Discussionmentioning

confidence: 99%

Rapid Screening for Freshwater Bacterial Groups by Using Reverse Line Blot Hybridization

Zwart

Hannen

Agterveld

et al. 2003

Appl Environ Microbiol

102

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“…It has been identified in several outbreaks due to the contamination of intravenous products, 'sterile' water, saline, chlorhexidene solutions, respiratory therapy solutions and intravenous catheters [56][57][58]. It has also been associated with pseudobacteraemias and asymptomatic colonisation [59,60]. R. pickettii may be recovered from the respiratory tract of patients with cystic fibrosis [61].…”

Section: Ralstonia Pickettiimentioning

confidence: 99%

Antimicrobial susceptibility of uncommonly isolated non-enteric Gram-negative bacilli

Sader

Jones

2005

International Journal of Antimicrobial Agents

164

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“…Both bacteria are ubiquitous in the environment but rarely cause symptomatic infection in healthy individuals [29]. However, they have been associated with nosocomial infections [30][31][32][33] and with infections of the respiratory system in patients with cystic fibrosis [34][35][36]. One can hypothesize that the in vitro immunization with gag peptides led to the priming of naive cells that cross-react with peptides from these two pathogens.…”

Section: S a R W N T P T A M32253mentioning

confidence: 99%

Identification of peptides from human pathogens able to cross‐activate an HIV‐1‐gag‐specific CD4⁺ T cell clone

Venturini¹,

Allicotti²,

Zhao³

et al. 2005

Eur J Immunol

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Antigen recognition by T cells is degenerate both at the MHC and the TCR level. In this study, we analyzed the cross‐reactivity of a human HIV‐1 gag p24‐specific CD4+ T cell clone obtained from an HIV‐1‐seronegative donor using a positional scanning synthetic combinatorial peptide library (PS‐SCL)‐based biometrical analysis. A number of decapeptides able to activate the HIV‐1 gag‐specific clone were identified and shown to correspond to sequences found in other human pathogens. Two of these peptides activated the T cell clone with the same stimulatory potency as the original HIV‐1 gag p24 peptide. These findings show that an HIV‐1‐specific human T helper clone can react efficiently with peptides from other pathogens and suggest that cellular immune responses identified as being specific for one human pathogen (HIV‐1) could arise from exposure to other pathogens.

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Ralstonia pickettii colonization of patients in an obstetric ward caused by a contaminated irrigation system

Cited by 18 publications

References 1 publication

Rapid Screening for Freshwater Bacterial Groups by Using Reverse Line Blot Hybridization

Rapid Screening for Freshwater Bacterial Groups by Using Reverse Line Blot Hybridization

Antimicrobial susceptibility of uncommonly isolated non-enteric Gram-negative bacilli

Identification of peptides from human pathogens able to cross‐activate an HIV‐1‐gag‐specific CD4⁺ T cell clone

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Ralstonia pickettii colonization of patients in an obstetric ward caused by a contaminated irrigation system

Cited by 18 publications

References 1 publication

Rapid Screening for Freshwater Bacterial Groups by Using Reverse Line Blot Hybridization

Rapid Screening for Freshwater Bacterial Groups by Using Reverse Line Blot Hybridization

Antimicrobial susceptibility of uncommonly isolated non-enteric Gram-negative bacilli

Identification of peptides from human pathogens able to cross‐activate an HIV‐1‐gag‐specific CD4+ T cell clone

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Identification of peptides from human pathogens able to cross‐activate an HIV‐1‐gag‐specific CD4⁺ T cell clone