Raise and characterization of a bread wheat hybrid line (Tulaykovskaya 10 × Saratovskaya 29) with chromosome 6Agi2 introgressed from Thinopyrum intermedium

Abstract

Wheatgrass Thinopyrum intermedium is a source of agronomically valuable traits for common wheat. Partial wheat–wheatgrass amphidiploids and lines with wheatgrass chromosome substitutions are extensively used as intermediates in breeding programs. Line Agis 1 (6Agi2/6D) is present in the cultivar Tulaykovskaya 10 pedigree. Wheatgrass chromosome 6Agi2 carries multiple resistance to fungal diseases in various ecogeographical zones. In this work, we studied the transfer of chromosome 6Agi2 in hybrid populations Saratovskaya 29×Tulaykovskaya 10 (S29×T10) and Tulaykovskaya 10×Saratovskaya 29 (T10×S29). Chromosome 6Agi2 was identified by PCR with chromosome-specific primers and by genomic in situ hybridization (GISH). According to molecular data, 6Agi2 was transmitted to nearly half of the plants tested in the F2 and F3 generations. A new breeding line 49-14 (2n = 42) with chromosome pair 6Agi2 was isolated and characterized in T10×S29 F5 by GISH. According to the results of our field experiment in 2020, the line had high productivity traits. The grain weights per plant (10.04±0.93 g) and the number of grains per plant (259.36±22.49) did not differ significantly from the parent varieties. The number of grains per spikelet in the main spike was significantly higher than in S29 (p ≤ 0.001) or T10 (p ≤ 0.05). Plants were characterized by the ability to set 3.77±0.1 grains per spikelet, and this trait varied among individuals from 2.93 to 4.62. The grain protein content was 17.91 %, and the gluten content, 40.55 %. According to the screening for fungal disease resistance carried out in the field in 2018 and 2020, chromosome 6Agi2 makes plants retain immunity to the West Siberian population of brown rust and to dominant races of stem rust. It also provides medium resistant and medium susceptible types of response to yellow rust. The possibility of using lines/varieties of bread wheat with wheatgrass chromosomes 6Agi2 in breeding in order to increase protein content in the grain, to confer resistance to leaf diseases on plants and to create multiflowered forms is discussed.

Resistance of Modern Russian Winter Wheat Cultivars to Yellow Rust

“…A similar situation with yellow rust incidence has been observed in the region of Central Chernozem [21]. The disease is observed sporadically in the Western Siberia and Volga region on spring wheat [19,20].…”

“…Over the last 5 years, the significance of Pst has increased markedly in other Russian regions. The disease has appeared in the regions North-West [18], West Siberia [19], Volga [20] and Central Chernozem regions [21]. Global climate changes contribute to changes in disease incidence and severity.…”

Genome Analysis of Thinopyrum intermedium and Its Potential Progenitor Species Using Oligo-FISH

“…Dewey; 2n = 6x = 42) is an important worldwide forage crop and a valuable gene reservoir for wheat improvement [1][2][3]. It has contributed many desirable traits such as resistance to three wheat rust diseases (leaf, stem, and stripe rust), barley yellow dwarf virus (BYDV), and wheat streak mosaic virus (WSMV), and grain quality for wheat improvement [3][4][5][6][7][8]. The IWG has also been developed into a perennial grain crop, named "Kernza" [9][10][11].…”

Virulence Diversity of Puccinia striiformis f. sp. Tritici in Common Wheat in Russian Regions in 2019–2021

“…However, significance of Pst remarkably increased over the last 5 years. The disease has appeared regularly in other Russian regions (North-West, West Siberia, Volga, and Central Black Earth) [29][30][31]. Global climate changes contribute to alteration of the disease incidence and severity.…”