Rainbow trout (Oncorhynchus mykiss) recombinant IL-1β and derived peptides induce migration of head-kidney leucocytes in vitro

Peddie, Scott; Zou, Jun; Cunningham, Charles; Secombes, Christopher J.

doi:10.1006/fsim.2001.0348

Cited by 63 publications

(38 citation statements)

References 31 publications

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“…P3 corresponded to fragment 197-206 (YRRNTGVDIS) of the trout sequence and had a MW of 1.18 kDa. Additional details of each peptide are presented in Peddie et al (2001).…”

Section: Methodsmentioning

confidence: 99%

“…In vivo studies have confirmed that intraperitoneally administered rIL-1β enhances phagocytosis and leucocyte migration in the peritoneal cavity, in addition to increasing IL-1β, COX-2 and lysozyme II gene expression in head kidney and gill tissue (Hong et al in press). Moreoever, rIL-1β has also been shown to enhance resistance to the bacterial pathogen Aeromonas salmonicida, both in vitro (Peddie 2002) and in vivo (Hong et al 2003).…”

Section: Recent Studies Have Demonstrated That Rainbow Troutmentioning

confidence: 99%

“…P3 corresponded to fragment 197-206 (YRRNTGVDIS) of the trout sequence and had a MW of 1.18 kDa. Additional details of each peptide are presented in Peddie et al (2001).Peptides were dissolved in 10 ml sterile 0.15 M phosphate buffered saline (PBS, Gibco), pH 7.2, and sonicated on ice. Stock solutions (10 mM) were stored in 0.5 ml aliquots at -80°C until required.…”

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confidence: 99%

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Effect of intraperitoneally administered IL-1b-derived peptides on resistance to viral haemorrhagic septicaemia in rainbow trout Oncorhynchus mykiss

Peddie¹,

McLauchlan²,

Ellis³

et al. 2003

Dis. Aquat. Org.

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The present work provides the first information concerning the immunostimulatory activity of trout interleukin (IL)-1β-derived peptides in vivo. Previous studies have demonstrated the ability of 2 such peptides, referred to as P1 and P3, to up-regulate a range of important immune parameters in vitro. P1 corresponds to fragment 146-157 (YVTPVPIETEAR) of the trout sequence and is analogous to a biologically active mammalian IL-1β-derived peptide, whilst P3 was synthesised to complex with the IL-1 receptor and corresponds to fragment 197-206 (YRRNTGVDIS) of the trout sequence. Optimal migration of peritoneal leucocytes, peptide induced phagocytosis and intracellular respiratory burst activity occurred following intraperitoneal injection of 3.0 µmol of P3. Furthermore, resistance to viral haemorrhagic septicaemia virus (VHSV) was soon augmented (2 d) post-injection of P3.KEY WORDS: Interleukin-1β · Phagocytosis · Respiratory burst · Disease resistance · Immunomodulation · VHS 56: 195-200, 2003 cytes (Peddie et al. 2002a). Although bioactive in its own right, the ability of P1 to enhance leucocyte migration and phagocytosis is enhanced when it is combined with P3 (Peddie et al. , 2002a. Resale or republication not permitted without written consent of the publisherDis Aquat OrgThe aim of this paper is to investigate the ability of IL-1β-derived peptides, particularly P3, to enhance leucocyte migration, phagocytic activity and superoxide anion production in the peritoneal cavity of rainbow trout. Furthermore, the ability of these peptides to enhance resistance to viral haemorrhagic septicaemia virus (VHSV) is ascertained. MATERIALS AND METHODSProduction of peptides. Three peptides, referred to as P1, P2 and P3, were synthesised at the Proteome Facility, University of Aberdeen, Scotland, UK. P1 corresponded to fragment 146-157 (YVTPVPIETEAR) of the trout IL-1β sequence (Zou et al. 1999), and had a molecular weight (MW) of 1.37 kDa. P2 was used as an immunologically irrelevant peptide and consisted of the same 12 amino acids as P1, but arranged in a random sequence (VVEEYIRAPPTT). P3 corresponded to fragment 197-206 (YRRNTGVDIS) of the trout sequence and had a MW of 1.18 kDa. Additional details of each peptide are presented in Peddie et al. (2001).Peptides were dissolved in 10 ml sterile 0.15 M phosphate buffered saline (PBS, Gibco), pH 7.2, and sonicated on ice. Stock solutions (10 mM) were stored in 0.5 ml aliquots at -80°C until required. Final concentrations were obtained by diluting the necessary volume of stock solution in sterile PBS.Fish maintenance. Rainbow trout weighing 100 to 500 g were obtained from Almondbank trout farm (Perthshire, UK), stocked in 250 l tanks at the Zoology Department, University of Aberdeen, maintained at 12 ± 1°C, with a constant flow of aerated and dechlorinated mains water. For the VHSV challenge experiment, trout (average weight 10 g) were maintained at the SEERAD Marine Laboratory, Aberdeen. Fish were fed ad libitum daily with commercial pellets (Ewos). An acclimation pe...

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“…P3 corresponded to fragment 197-206 (YRRNTGVDIS) of the trout sequence and had a MW of 1.18 kDa. Additional details of each peptide are presented in Peddie et al (2001).…”

Section: Methodsmentioning

confidence: 99%

Section: Recent Studies Have Demonstrated That Rainbow Troutmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Effect of intraperitoneally administered IL-1b-derived peptides on resistance to viral haemorrhagic septicaemia in rainbow trout Oncorhynchus mykiss

Peddie¹,

McLauchlan²,

Ellis³

et al. 2003

Dis. Aquat. Org.

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“…The chemotaxis assay was performed using rainbow trout HK primary leucocytes as described previously (Peddie et al, 2001). …”

Section: Chemotactic Activity Of CXC Chemokinesmentioning

confidence: 99%

Phylogenetic analysis of vertebrate CXC chemokines reveals novel lineage specific groups in teleost fish

Chen

Wang

et al. 2013

Developmental & Comparative Immunology

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“…At 24 h after IL-1b challenge, total RNA from liver, kidney, spleen, and head kidney leukocytes was isolated and transcribed into cDNA. The head kidney leukocytes of Tetraodon were isolated as described previously (67). Briefly, the head kidney was removed from fish under sterile conditions, washed twice with L-15 medium, and gently passed through a 100-mm nylon mesh.…”

Section: Evaluation Of Digirr In Il-1-induced Inflammationmentioning

confidence: 99%

Discovery of the DIGIRR Gene from Teleost Fish: A Novel Toll–IL-1 Receptor Family Member Serving as a Negative Regulator of IL-1 Signaling

Jin

et al. 2011

The Journal of Immunology

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Toll–IL-1R (TIR) family members play crucial roles in a variety of defense, inflammatory, injury, and stress responses. Although they have been widely investigated in mammals, little is known about TIRs in ancient vertebrates. In this study, we report a novel double Ig IL-1R related molecule (DIGIRR) from three model fish (Tetraodon nigroviridis, Gasterosteus aculeatus, and Takifugu rubripes), adding a previously unknown homolog to the TIR family. This DIGIRR molecule contains two Ig-like domains in the extracellular region, one Arg-Tyr–mutated TIR domain in the intracellular region, and a unique subcellular distribution within the Golgi apparatus. These characteristics distinguish DIGIRR from other known family members. In vitro injection of DIGIRR into zebrafish embryos dramatically inhibited LPS-induced and IL-1β–induced NF-κB activation. Moreover, in vivo knockdown of DIGIRR by small interfering RNA significantly promoted the expression of IL-1β–stimulated proinflammatory cytokines (IL-6 and IL-1β) in DIGIRR-silenced liver and kidney tissues and in leukocytes. These results strongly suggest that DIGIRR is an important negative regulator of LPS-mediated and IL-1β–mediated signaling pathways and inflammatory responses. The Arg-Tyr–mutated site disrupted the signal transduction ability of DIGIRR TIR. Evolutionally, we propose a hypothesis that DIGIRR and single Ig IL-1R related molecule (SIGIRR) might originate from a common ancient IL-1R–like molecule that lost one (in DIGIRR) or two (in SIGIRR) extracellular Ig-like domains and intracellular Ser and Arg-Tyr amino acids. DIGIRR might be an evolutionary “transitional molecule” between IL-1R and SIGIRR, representing a shift from a potent receptor to a negative regulator. These results help define the evolutionary history of TIR family members and their associated signaling pathways and mechanisms.

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Rainbow trout (Oncorhynchus mykiss) recombinant IL-1β and derived peptides induce migration of head-kidney leucocytes in vitro

Cited by 63 publications

References 31 publications

Effect of intraperitoneally administered IL-1b-derived peptides on resistance to viral haemorrhagic septicaemia in rainbow trout Oncorhynchus mykiss

Effect of intraperitoneally administered IL-1b-derived peptides on resistance to viral haemorrhagic septicaemia in rainbow trout Oncorhynchus mykiss

Phylogenetic analysis of vertebrate CXC chemokines reveals novel lineage specific groups in teleost fish

Discovery of the DIGIRR Gene from Teleost Fish: A Novel Toll–IL-1 Receptor Family Member Serving as a Negative Regulator of IL-1 Signaling

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