RAF-like protein kinases mediate a deeply conserved, rapid auxin response

Kuhn, Andre; Roosjen, Mark; Mutte, Sumanth; Dubey, Shiv Mani; Carrillo Carrasco, Vanessa Polet; Boeren, Sjef; Monzer, Aline; Koehorst, Jasper; Kohchi, Takayuki; Nishihama, Ryuichi; Fendrych, Matyáš; Sprakel, Joris; Friml, Jiří; Weijers, Dolf

doi:10.1016/j.cell.2023.11.021

Cited by 25 publications

(7 citation statements)

References 135 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Owing to the intensified efforts to understand the origin of land plants, it becomes apparent that many of the key traits of land plants have deep evolutionary roots (Hori et al, 2014;Delaux et al, 2015;Feng et al, 2023;Dadras et al, 2023b;Bierenbroodspot et al, 2024;Kuhn et al, 2024)-and chemodiversity is a point in case. One major contributor to the specialized metabolism that makes up embryophytic chemodiversity is the CYPs.…”

Section: Discussionmentioning

confidence: 99%

Sequence similarity networks bear out hierarchical relationships of green cytochrome P450

Dhabalia Ashok,

Freitag,

Irisarri

et al. 2024

Physiologia Plantarum

View full text Add to dashboard Cite

Land plants have diversified enzyme families. One of the most prominent is the cytochrome P450 (CYP or CYP450) family. With over 443,000 CYP proteins sequenced across the tree of life, CYPs are ubiquitous in archaea, bacteria, and eukaryotes. Here, we focused on land plants and algae to study the role of CYP diversification. CYPs, acting as monooxygenases, catalyze hydroxylation reactions crucial for specialized plant metabolic pathways, including detoxification and phytohormone production; the CYPome consists of one enormous superfamily that is divided into clans and families. Their evolutionary history speaks of high substrate promiscuity; radiation and functional diversification have yielded numerous CYP families. To understand the evolutionary relationships within the CYPs, we employed sequence similarity network analyses. We recovered distinct clusters representing different CYP families, reflecting their diversified sequences that we link to the prediction of functionalities. Hierarchical clustering and phylogenetic analysis further elucidated relationships between CYP clans, uncovering their shared deep evolutionary history. We explored the distribution and diversification of CYP subfamilies across plant and algal lineages, uncovering novel candidates and providing insights into the evolution of these enzyme families. This identified unexpected relationships between CYP families, such as the link between CYP82 and CYP74, shedding light on their roles in plant defense signaling pathways. Our approach provides a methodology that brings insights into the emergence of new functions within the CYP450 family, contributing to the evolutionary history of plants and algae. These insights can be further validated and implemented via experimental setups under various external conditions.

show abstract

Section: Discussionmentioning

confidence: 99%

Sequence similarity networks bear out hierarchical relationships of green cytochrome P450

Dhabalia Ashok,

Freitag,

Irisarri

et al. 2024

Physiologia Plantarum

View full text Add to dashboard Cite

show abstract

“…Recent research has revealed that both charophytes and land plants utilize OPDA and dnOPDA in a thermotolerance response, which is distinct from the role of these compounds in jasmonate signaling that only evolved in land plants 15 , 45 . Canonical auxin signaling is likewise a land plant invention 77 , yet applied IAA elicits rapid changes in plasma membrane potential and protein phosphorylation in both land plants and charophytes; while the native function in charophytes remains unknown, it may involve a reaction to light or osmotic stress 80 . As charophytes become better explored and established as model organisms, we are likely to be updating our views regarding the evolution and origins of phytohormone responses.…”

Section: Discussionmentioning

confidence: 99%

Phytohormone profiling in an evolutionary framework

Schmidt,

Skokan,

Depaepe

et al. 2024

Nat Commun

View full text Add to dashboard Cite

The genomes of charophyte green algae, close relatives of land plants, typically do not show signs of developmental regulation by phytohormones. However, scattered reports of endogenous phytohormone production in these organisms exist. We performed a comprehensive analysis of multiple phytohormones in Viridiplantae, focusing mainly on charophytes. We show that auxin, salicylic acid, ethylene and tRNA-derived cytokinins including cis-zeatin are found ubiquitously in Viridiplantae. By contrast, land plants but not green algae contain the trans-zeatin type cytokinins as well as auxin and cytokinin conjugates. Charophytes occasionally produce jasmonates and abscisic acid, whereas the latter is detected consistently in land plants. Several phytohormones are excreted into the culture medium, including auxin by charophytes and cytokinins and salicylic acid by Viridiplantae in general. We note that the conservation of phytohormone biosynthesis and signaling pathways known from angiosperms does not match the capacity for phytohormone biosynthesis in Viridiplantae. Our phylogenetically guided analysis of established algal cultures provides an important insight into phytohormone biosynthesis and metabolism across Streptophyta.

show abstract

“…ABL1 and ABL2 regulate rapid auxin responses and various plant developmental processes through distinct and overlapping functions with ABP1. 4 To verify roles of ABP1 in auxin responses, a dominant negative form of ABP1-5 was overexpressed in a null abp1-TD1 mutant. Similar to the tmk1 tmk4, tmk1 tmk2 tmk3 tmk4, and pin1 mutants, the ABP1-5;abp1 transgenic line exhibits a series of developmental deficiencies and shows impaired auxin responses, indicating that accumulation of the mutant protein ABP1-5 suppresses TMK-modulated auxin responses.…”

Section: Complexesmentioning

confidence: 99%

“…Importantly, the TMK1-ex also performed specific auxin binding activity, and the mixture of TMK1-ex with ABP1 or ABL1 exhibited significantly greater IAA binding affinity than ABP1/ABL1 or TMK1-ex itself, demonstrating that TMK1 and ABP1/ABL1 are co-receptors of apoplastic auxin in plants. 4…”

Section: Complexesmentioning

confidence: 99%

ABP1/ABLs and TMKs form receptor complexes to perceive extracellular auxin and trigger fast phosphorylation responses

Cheng,

Li,

Wang

2024

TIL

View full text Add to dashboard Cite

RAF-like protein kinases mediate a deeply conserved, rapid auxin response

Cited by 25 publications

References 135 publications

Sequence similarity networks bear out hierarchical relationships of green cytochrome P450

Sequence similarity networks bear out hierarchical relationships of green cytochrome P450

Phytohormone profiling in an evolutionary framework

ABP1/ABLs and TMKs form receptor complexes to perceive extracellular auxin and trigger fast phosphorylation responses

Contact Info

Product

Resources

About