Raf: A key regulatory kinase for transduction of mitogenic and metabolic signals of the probiotic Saccharomyces boulardii

Buts, Jean-Paul; Dekeyser, Nadine

doi:10.1016/j.clinre.2011.04.010

Cited by 3 publications

(4 citation statements)

References 10 publications

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“…It exerts trophic effects such as stimulation of brush border membrane digestive enzymes and nutrient transporter activity [74]. Several studies have shown a wide array of trophic effects stimulated by S. boulardii: brush border sucrase, lactase, and maltase activities [44,[75][76][77][78]; iso-maltase activity [78]; glucoamylase and N-aminopeptidase activity [76]; leucine-aminopeptidase activity [79]; α,α-trehalase activities in the endoluminal fluid and intestinal mucosa; brush border α-glucosidase [80]; spermine, spermidine and putrescine levels in rat jejunal mucosa [75,77]; adenosine triphosphatase, γ-glutamyl transpeptidase, lipase, and trypsin activities and TNF-α, IL-10, transforming growth factor beta (TGF-β), and secretory IgA [5]; diamine oxidase activities, brush border sodium/glucose cotransporter 1 expression and sodium-dependent glucose uptake [74,77]; GRB2-SHC-CrkII-Ras-GAP-Raf-ERK1,2 transduction pathway in rats and decreased p38 MAPK and NF-κB [81][82][83].…”

Section: Trophic Effectsmentioning

confidence: 99%

Saccharomyces boulardii: What Makes It Tick as Successful Probiotic?

Pais

Almeida

Yılmaz

et al. 2020

JoF

146

101

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Saccharomyces boulardii is a probiotic yeast often used for the treatment of GI tract disorders such as diarrhea symptoms. It is genetically close to the model yeast Saccharomyces cerevisiae and its classification as a distinct species or a S. cerevisiae variant has long been discussed. Here, we review the main genetic divergencies between S. boulardii and S. cerevisiae as a strategy to uncover the ability to adapt to the host physiological conditions by the probiotic. S. boulardii does possess discernible phenotypic traits and physiological properties that underlie its success as probiotic, such as optimal growth temperature, resistance to the gastric environment and viability at low pH. Its probiotic activity has been elucidated as a conjunction of multiple pathways, ranging from improvement of gut barrier function, pathogen competitive exclusion, production of antimicrobial peptides, immune modulation, and trophic effects. This review summarizes the participation of S. boulardii in these mechanisms and the multifactorial nature by which this yeast modulates the host microbiome and intestinal function.

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Section: Trophic Effectsmentioning

confidence: 99%

Saccharomyces boulardii: What Makes It Tick as Successful Probiotic?

Pais

Almeida

Yılmaz

et al. 2020

JoF

146

101

View full text Add to dashboard Cite

show abstract

“…In experiments using immunoprecipitation and immunoblotting of preparations from rat intestinal tissues, it was concluded that S. boulardii CNCM I-745 acts via the pathway GRB2-SHC-CrkII-Ras-GAP-Raf-ERK1,2. 84 , 85 This MAPK pathway is known to control cellular proliferation, differentiation, and survival and may also be relevant for the upregulation of enzyme activity.…”

Section: S Boulardii Cncm I-745–induced Mitogenic and Metmentioning

confidence: 99%

“…The MAPK pathway gets activated in response to S. boulardii. 84 , 85 LPH, lactase-phlorizin hydrolase – digestive enzyme with 2 domains, one splitting, among others, lactose, cellobiose o-nitrophenyl- β-glucopyranoside, and o-nitrophenyl- β-galactopyranoside, and the other splitting, among others, phlorizin, β-glycopyranosylceramides, and m-nitrophenyl- β-glucopyranoside. 98 Upregulated in response to S. boulardii.…”

Section: S Boulardii Cncm I-745–induced Mitogenic and Metmentioning

confidence: 99%

“…Also, S. boulardii CNCM I-745 decreased activation of p38 MAPK, a kinase responsive to pro-inflammatory cytokines and environmental stress. 84 , 85 , 88 More studies in this area will improve the precise mechanistic understanding of digestive enzyme regulation by S. boulardii CNCM I-745.…”

Section: S Boulardii Cncm I-745–induced Mitogenic and Metmentioning

confidence: 99%

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Saccharomyces boulardii CNCM I-745 Improves Intestinal Enzyme Function: A Trophic Effects Review

Moré

Vandenplas

2018

Clin Med Insights Gastroenterol

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Several properties of the probiotic medicinal yeast Saccharomyces boulardii CNCM I-745 contribute to its efficacy to prevent or treat diarrhoea. Besides immunologic effects, pathogen-binding and anti-toxin effects, as well as positive effects on the microbiota, S boulardii CNCM I-745 also has pronounced effects on digestive enzymes of the brush border membrane, known as trophic effects. The latter are the focus of this review. Literature has been reviewed after searching Medline and PMC databases. All relevant non-clinical and clinical studies are summarized. S. boulardii CNCM I-745 synthesizes and secretes polyamines, which have a role in cell proliferation and differentiation. The administration of polyamines or S. boulardii CNCM I-745 enhances the expression of intestinal digestive enzymes as well as nutrient uptake transporters. The signalling mechanisms leading to enzyme activation are not fully understood. However, polyamines have direct nucleic acid–binding capacity with regulatory impact. S. boulardii CNCM I-745 induces signalling via the mitogen-activated protein kinase pathway. In addition, effects on the phosphatidylinositol-3 kinase (PI3K) pathway have been reported. As an additional direct effect, S. boulardii CNCM I-745 secretes certain enzymes, which enhance nutrient acquisition for the yeast and the host. The increased availability of digestive enzymes seems to be one of the mechanisms by which S. boulardii CNCM I-745 counteracts diarrhoea; however, also people with certain enzyme deficiencies may profit from its administration. More studies are needed to fully understand the mechanisms of trophic activation by the probiotic yeast.

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Saccharomyces boulardii CNCM I-745 — die medizinische Hefe verbessert die Funktion intestinaler Enzyme

Moré

2019

MMW - Fortschritte der Medizin

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Raf: A key regulatory kinase for transduction of mitogenic and metabolic signals of the probiotic Saccharomyces boulardii

Cited by 3 publications

References 10 publications

Saccharomyces boulardii: What Makes It Tick as Successful Probiotic?

Saccharomyces boulardii: What Makes It Tick as Successful Probiotic?

Saccharomyces boulardii CNCM I-745 Improves Intestinal Enzyme Function: A Trophic Effects Review

Saccharomyces boulardii CNCM I-745 — die medizinische Hefe verbessert die Funktion intestinaler Enzyme

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