Radix Pueraria Flavonoids Assisted Green Synthesis of Reduced Gold Nanoparticles: Application for Electrochemical Nonenzymatic Detection of Cholesterol in Food Samples

Abstract

Using radix pueraria flavonoids (RPFs) as a reducing and stabilizing agent, we report a simple, cost-effective, and ecologically friendly green synthesis technique for gold nanoparticles (AuNPs) in the present study. Ultraviolet−visible (UV) spectroscopy, transmission electron microscopy (TEM), Fourier transform infrared (FTIR), and X-ray diffraction (XRD) investigations were used to characterize the AuNPs. The results demonstrated that the produced AuNPs were nearly spherical and that their particle sizes had a mean diameter of 4.85 ± 0.75 nm. The "Green" AuNPs, exhibiting remarkable peroxidase-like activity and Michaelis−Menten kinetics with high affinity for H 2 O 2 and 3,3′,5,5′-tetramethylbenzidine (TMB), were effectively applied to the fabrication of a sensitive nonenzymatic enhanced electrochemical sensor for the detection of cholesterol (Cho). Under optimum circumstances, it was possible to establish two linear ranges of 1−100 and 250−5000 μmol/L with a detection limit of 0.259 μmol/L (signal/noise ratio (S/N) = 3). The suggested sensor was utilized with satisfactory findings to determine the amount of Cho in food samples.

Biosensors in Food and Healthcare Industries: Bio-Coatings Based on Biogenic Nanoparticles and Biopolymers

“…al. [143] described the green synthesis of reduced gold nanoparticles (AuNPs) using the extract of Radix Pueraria flavonoids as a reducing and stabilising agent. The performance of the synthesised AuNPs was evaluated for their electrochemical sensing applications, particularly for the non-enzymatic detection of cholesterol in food samples.…”

The impact of pH and temperature on the green gold nanoparticles preparation using Jeju Hallabong peel extract for biomedical applications

“…In general, the green synthesis method, also known as biosynthesis, employs natural resources such as microorganisms, viruses, fungi, algae, and plant extracts to facilitate the production of nanoparticles. [17][18][19][20][21] Plant extracts, in particular, have demonstrated efficacy as stabilizing and capping agents in the synthesis of AuNPs. This is attributed to the presence of bioactive compositions such as sugars, phenolic compounds, alkaloids, avonoids, green terpenoids, and proteins, which aid in the synthesis of AuNPs with desirable characteristics such as ne shapes, size, and biocompatibility.…”

Comparison of Blueberry and Dandelion Aqueous Extracts in the Synthesis of Gold and Silver Nanostructures, and Their Applications as Anti‐Pseudomonas aeruginosa Agent

“…Metallic nanostructures (MNSs) are under massive investigation owing to their unlimited applications from biomedical sciences [1] to electronics [2] . Despite of the fact that precise control on size, morphology and even crystallinity of the MNSs have been achieved using synthetic approaches, [3,4] still their precisely controlled synthesis by sustainable and green methods are challenging.…”

“…Metallic nanostructures (MNSs) are under massive investigation owing to their unlimited applications from biomedical sciences [1] to electronics. [2] Despite of the fact that precise control on size, morphology and even crystallinity of the MNSs have been achieved using synthetic approaches, [3,4] still their precisely controlled synthesis by sustainable and green methods are challenging. Such green-approaches as plant extracts, [2,5] microorganisms, [6] benign organic compounds [7,8] and physical methods [9] have been proposed to improve the sustainable MNSs production.…”

“…Despite of the fact that precise control on size, morphology and even crystallinity of the MNSs have been achieved using synthetic approaches, [3,4] still their precisely controlled synthesis by sustainable and green methods are challenging. Such green‐approaches as plant extracts, [2,5] microorganisms, [6] benign organic compounds [7,8] and physical methods [9] have been proposed to improve the sustainable MNSs production. Particularly, plant extracts provide synthesis of unique shaped and sized MNSs [10–12] .…”