Radix Glycyrrhizae extract and licochalcone a exert an anti-inflammatory action by direct suppression of toll like receptor 4

Cai, Min; Xu, Youcai; Deng, Bo; Chen, Jun-Bang; Chen, Ting-Fang; Zeng, Ke-Feng; Chen, Si; Deng, Suihui; Tan, Zhenghuai; Ding, Wenjun; Zhang, Shuang-wei; Liu, Bin; Zhang, Jingzhi

doi:10.1016/j.jep.2022.115869

Cited by 12 publications

(3 citation statements)

References 51 publications

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“…All molecular dynamics (MD) simulations were performed using AMBER 03 force field. Next, the best conformation of STAT3-IATL complex was selected for MD stimulation using YASARA as previously described (Cai et al, 2023).…”

Section: Molecular Docking and Molecular Dynamicsmentioning

confidence: 99%

Isoalantolactone exerts anti‐melanoma effects via inhibiting PI3K/AKT/mTOR and STAT3 signaling in cell and mouse models

Li,

Jiang,

Zhang

et al. 2024

Phytotherapy Research

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Background and AimAlthough the anti‐cancer activity of isoalantolactone (IATL) has been extensively studied, the anti‐melanoma effects of IATL are still unknown. Here, we have investigated the anti‐melanoma effects and mechanism of action of IATL. MTT and crystal violet staining assays were performed to detect the inhibitory effect of IATL on melanoma cell viability. Apoptosis and cell cycle arrest induced by IATL were examined using flow cytometry. The molecular mechanism of IATL was explored by Western blotting, confocal microscope analysis, molecular docking, and cellular thermal shift assay (CETSA). A B16F10 allograft mouse model was constructed to determine the anti‐melanoma effects of IATL in vivo. The results showed that IATL exerted anti‐melanoma effects in vitro and in vivo. IATL induced cytoprotective autophagy in melanoma cells by inhibiting the PI3K/AKT/mTOR signaling. Moreover, IATL inhibited STAT3 activation both in melanoma cells and allograft tumors not only by binding to the SH2 domain of STAT3 but also by suppressing the activity of its upstream kinase Src. These findings demonstrate that IATL exerts anti‐melanoma effects via inhibiting the STAT3 and PI3K/AKT/mTOR signaling pathways, and provides a pharmacological basis for developing IATL as a novel phytotherapeutic agent for treating melanoma clinically.

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Section: Molecular Docking and Molecular Dynamicsmentioning

confidence: 99%

Isoalantolactone exerts anti‐melanoma effects via inhibiting PI3K/AKT/mTOR and STAT3 signaling in cell and mouse models

Li,

Jiang,

Zhang

et al. 2024

Phytotherapy Research

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“…However, concerns regarding the adverse effects and potential health risks associated with these synthetic bioactive compounds have prompted a shift toward exploring natural alternatives . Numerous studies have been conducted on the extraction of active substances with anti-inflammatory − and antioxidant properties. − Zingiber officinale, also known as ginger, is a readily available plant that has been studied for this purpose. Zingiber Officinale var.…”

Section: Introductionmentioning

confidence: 99%

Development of Nanostructured Lipid Carrier-Loaded Flavonoid-Enriched Zingiber officinale

Shazwani,

Marlina,

Misran

2024

ACS Omega

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Flavonoids, which are bioactive molecules found in Zingiber officinale, have been widely used as antioxidant and antiinflammatory drugs. The presence of nanostructured lipid carriers (NLCs) as sophisticated delivery systems for bioactive compounds, such as flavonoids, can increase their bioavailability and stability, thus potentially producing better therapeutic effects. This study aimed to develop an anti-inflammatory topical gel using NLC-containing flavonoids derived from Zingiber officinale. The NLC formulation was prepared using stearic acid, a mixture of medium-chain triglycerides and isopropyl myristate, Tween 20, and Span 20 by using a hot homogenization method. The total flavonoid content obtained through sequential maceration stages was 4.04 mg of QUE/g of dry extract. The highest encapsulation efficiency of flavonoid-loaded NLC was observed at a flavonoid, Zingiber officinale extract (ZOE) concentration of 2%. It was found that a ZOE concentration of 0.4% provided excellent stability with a particle size of 302−344 nm and a polydispersity index of 0.14−0.23 after 28 days of observation. Morphological analysis of the ZOE-loaded NLC revealed a stable and well-developed formulation with a fairly uniform distribution. The presence of distinctive and uniformly distributed single particles suggests a promising alternative drug delivery system for conventional topical preparations. ZOE-loaded NLC gel showed solid-like properties and higher quality stability than the gel.

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“…To date, it has been discovered that LCA has excellent activity to inhibit the proliferation and motility of 17 classes of cancer cells [2,3]. Furthermore, LCA has demonstrated anti-inflammatory effects against both acute and chronic inflammation [4,5]. Moreover, LCA has been shown to have antioxidant activity and neuroprotective effects [6,7].…”

Section: Introductionmentioning

confidence: 99%

Histone Deacetylase GiSRT2 Negatively Regulates Flavonoid Biosynthesis in Glycyrrhiza inflata

Zeng,

Huang,

Zhou

et al. 2023

Cells

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Glycyrrhiza inflata Batalin is a medicinal licorice species that has been widely used by humans for centuries. Licochalcone A (LCA) is a characteristic flavonoid that accumulates in G. inflata roots with high economical value. However, the biosynthetic pathway and regulatory network of its accumulation remain largely unknown. Here we found that a histone deacetylase (HDAC) inhibitor nicotinamide (NIC) could enhance the accumulation of LCA and total flavonoids in G. inflata seedlings. GiSRT2, a NIC-targeted HDAC was functionally analyzed and its RNAi transgenic hairy roots accumulated much more LCA and total flavonoids than its OE lines and the controls, indicating a negative regulatory role of GiSRT2 in the accumulation of LCA and total flavonoids. Co-analysis of transcriptome and metabolome of RNAi-GiSRT2 lines revealed potential mechanisms in this process. An O-methyltransferase gene, GiLMT1 was up-regulated in RNAi-GiSRT2 lines and the encoded enzyme catalyzed an intermediate step in LCA biosynthesis pathway. Transgenic hairy roots of GiLMT1 proved that GiLMT1 is required for LCA accumulation. Together, this work highlights the critical role of GiSRT2 in the regulation of flavonoid biosynthesis and identifies GiLMT1 as a candidate gene for the biosynthesis of LCA with synthetic biology approaches.

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Radix Glycyrrhizae extract and licochalcone a exert an anti-inflammatory action by direct suppression of toll like receptor 4

Cited by 12 publications

References 51 publications

Isoalantolactone exerts anti‐melanoma effects via inhibiting PI3K/AKT/mTOR and STAT3 signaling in cell and mouse models

Isoalantolactone exerts anti‐melanoma effects via inhibiting PI3K/AKT/mTOR and STAT3 signaling in cell and mouse models

Development of Nanostructured Lipid Carrier-Loaded Flavonoid-Enriched Zingiber officinale

Histone Deacetylase GiSRT2 Negatively Regulates Flavonoid Biosynthesis in Glycyrrhiza inflata

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