Radix Cyathula officinalis Kuan inhibits arterial remodeling in spontaneously hypertensive rats

Abstract

There is still no resolution for arterial remodeling related with hypertension, though hypertension treatment has access to a number of pharmacological agents. The present study aimed at investigating the prevention of Cyathula officinalis Kuan's roots (C. officinalis Kuan) against in arterial remodeling in vitro. Spontaneously hypertensive rats (SHRs) were intragastrically administered 3, 6 or 12 g/kg C. officinalis Kuan or normal saline or enalapril (2.5 mg/kg) once a day for 8 weeks. Hematoxylin and eosin were used to measure blood pressure and stain carotid and arota. The serum concentration of nitric oxide (NO) was measured by NO assay kit (nitrate reductase method). The endothelin-1 transcriptional level, endothelial NO synthase of endothelium as well as angiotensin II receptor type 1 (AT1R) of aorta and carotid was tested by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and the protein level in aorta was also measured by western blotting. The blood pressure in SHR+enalapril, SHR+3 g/kg, SHR+6 g/kg and SHR+12 g/kg C. officinalis Kuan groups was significantly decreased at 4, 6 and 8 weeks post-treatment compared with SHR group. Different doses of C. officinalis Kuan and enalapril treatment showed aortic wall thinness and strengthened NO serum level, but made no impact on the transcriptional level of AT1R in aorta or endothelial NO synthase in carotid. It is suggested by such results that therapy by C. officinalis Kuan is able to fight against arterial remodeling, thus may provide a new means to treat arterial remodeling caused by hypertension.

Review of traditional uses, botany, chemistry, pharmacology, pharmacokinetics, and toxicology of Radix Cyathulae

“…Modern pharmacological studies showed that Achyranthes bidentata polysaccharides can promote cellular immunity and antitumor properties and reduce peripheral leukocyte reduction induced by cyclophosphamide [22]. CR also contains glycine, valine, citramalic acid, ribonic acid, pipecolinic acid, N -carbamylglutamate, gluconic acid, 3-hydroxypropionic, glutaric acid, cellobiose, lactic acid, gluconic acid, xylitol, glutaric acid, pipecolinic acid, ribonic acid, mannose, oxalic acid, digalacturonic acid, lactic acid, 2-deoxyerythritol, acetol, 3-hydroxybutyric acid, N -carbamylglutamate, cellobiose, and palmitic acid [23, 24].…”

JYYS Granule Mitigates Renal Injury in Clinic and in Spontaneously Hypertensive Rats by Inhibiting NF‐κB Signaling‐Mediated Microinflammation

“…JYYS granule has been a therapeutic drug for hypertension and early hypertension-caused renal damage by downregulating AngII and TNF- α level [32]. Extracts from Bidens pilosa, Cornus officinalis, Fallopia multiflora, Cyathula officinalis , and Alisma plantago-aquatica may ameliorate hypertension and renal injury [24–26] and show antioxidant activity [27], inhibit arterial remodeling in spontaneously hypertensive rats [28], and exhibit significant anti-chronic-prostatitis and anti-inflammatory activities [29–31]. In this study, the data also demonstrates that JYYS granule, in a dose dependent manner, could significantly decrease urinary albumin and β 2-MG excretion, and it ameliorates renal pathological changes and suppresses expression of profibrogenic genes and extracellular matrix genes including renal collagen-I, fibronectin, PAI-1, TGF β 1, TIMP-1, and collagen-IV.…”

“…Extracts from Fallopia multiflora show antioxidant activity [27]. Extracts from Cyathula officinalis inhibit arterial remodeling in spontaneously hypertensive rats [28]. Components from Alisma plantago-aquatica show significant anti-chronic-prostatitis activity, anti-inflammatory, and antioxidant activities [29–31].…”