Radiosensitizing properties of magnetic hyperthermia mediated by superparamagnetic iron oxide nanoparticles (SPIONs) on human cutaneous melanoma cell lines

Rybka, Jakub Dalibor

doi:10.1016/j.rpor.2019.01.002

Cited by 44 publications

(30 citation statements)

References 65 publications

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“…The RF heating is caused by Joule heating or magnetic heating 63. So far, there are no optimal conditions for effective RF-heating of NPs.…”

Section: Cancer Treatmentmentioning

confidence: 99%

<p>Emerging medical applications based on non-ionizing electromagnetic fields from 0 Hz to 10 THz</p>

Mattsson¹,

Simkó²

2019

MDER

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The potential for using non-ionizing electromagnetic fields (EMF; at frequencies from 0 Hz up to the THz range) for medical purposes has been of interest since many decades. A number of established and familiar methods are in use all over the world. This review, however, provides an overview of applications that already play some clinical role or are in earlier stages of development. The covered methods include modalities used for bone healing, cancer treatment, neurological conditions, and diathermy. In addition, certain other potential clinical areas are touched upon. Most of the reviewed technologies deal with therapy, whereas just a few diagnostic approaches are mentioned. None of the discussed methods are having such a strong impact in their field of use that they would be expected to replace conventional methods. Partly this is due to a knowledge base that lacks mechanistic explanations for EMF effects at low-intensity levels, which often are used in the applications. Thus, the possible optimal use of EMF approaches is restricted. Other reasons for the limited impact include a scarcity of well-performed randomized clinical trials that convincingly show the efficacy of the methods and that standardized user protocols are mostly lacking. Presently, it seems that some EMF-based methods can have a niche role in treatment and diagnostics of certain conditions, mostly as a complement to or in combination with other, more established, methods. Further development and a stronger impact of these technologies need a better understanding of the interaction mechanisms between EMF and biological systems at lower intensity levels. The importance of the different physical parameters of the EMF exposure needs also further investigations.

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“…The RF heating is caused by Joule heating or magnetic heating 63. So far, there are no optimal conditions for effective RF-heating of NPs.…”

Section: Cancer Treatmentmentioning

confidence: 99%

<p>Emerging medical applications based on non-ionizing electromagnetic fields from 0 Hz to 10 THz</p>

Mattsson¹,

Simkó²

2019

MDER

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“…Superparamagnetic Iron Oxide Nanoparticles (SPIONs) have been studied for a range of different treatments, from drug delivery to antibody-binding (48), however, they are primarily investigated for their ability to provide magnetic hyperthermia treatments (49)(50)(51)(52).…”

Section: Introductionmentioning

confidence: 99%

“…The superparamagnetic properties of SPIONs mean that, when placed in an alternating magnetic field, they vibrate and heat up, but once removed from the magnetic field, this magnetism is lost (50). When placed inside tissues, it is thought that this hyperthermia effect induces cell death (48).…”

Section: Introductionmentioning

confidence: 99%

Impact of Superparamagnetic Iron Oxide Nanoparticles on In Vitro and In Vivo Radiosensitisation of Cancer Cells

Russell

Dunne

Russell

et al. 2020

Preprint

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Purpose The recent implementation of MR-Linacs has highlighted theranostic opportunities of contrast agents in both imaging and radiotherapy. There is a lack of data exploring the potential of superparamagnetic iron oxide nanoparticles (SPIONs) as radiosensitisers. This study aimed to characterise the uptake and radiobiological effects of SPIONs in tumour cell models in vitro and to provide proof-of-principle application in a xenograft tumour model. Methods SPION uptake was measured using ICP-MS in 6 cancer cell lines; H460, MiaPaCa2, DU145, MCF7, U87 and HEPG2. The impact of SPIONs on radiobiological response was determined by measuring DNA damage using 53BP1 immunofluorescence and cell survival. Measured dose enhancement factors (DEFs) were with the predicted DEFs based on physical absorption estimations. In vivo efficacy was demonstrated using a subcutaneous H460 xenograft tumour model in SCID mice by following intra-tumoural injection of SPIONs. Results SPIONs significantly increased DNA damage in all cell lines with the exception of U87 cells at a dose of 1 Gy, 1 hr post-irradiation. Levels of DNA damage correlated with the cell survival, in which all cell lines except U87 cells showed an increased sensitivity (P < 0.05) in the linear quadratic curve fit for 1 hr exposure to 0.1 mM SPIONs. There was also a 30.1 % increase in the number of DNA damage foci found for HEPG2 cells at 2 Gy. No strong correlation was found between SPION uptake and DNA damage at any dose, yet the biological consequences of SPIONs on radiosensitisation were found to be much greater, with DEFs up to 1.28 ± 0.03, compared with predicted physical dose enhancement levels of 1.0001. In vivo , intra-tumoural injection of SPIONs combined with radiation showed significant tumour growth delay compared to animals treated with radiation or SPIONs alone (p < 0.05). Conclusions SPIONs showed radiosensitising effects in 5 out of 6 cancer cell lines. No correlation was found between the cell-specific uptake of SPIONs into the cells and DNA damage levels. The in vivo study found a significant decrease in the tumour growth rate.

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“…Magnetic iron oxide particles are used for in vitro diagnostics for nearly 40 years. Due to the unique physical, chemical, thermal and mechanical properties of iron oxide nanoparticles, as well as their biocompatibility and low toxicity in the human body, they have been used in many biomedical applications [1][2][3][4][5][6], such as contrast agents [7] for magnetic resonance imaging (MRI), carriers for controlled drug delivery and immunoassays [8][9][10][11][12][13], and also in magnetic hyperthermia [14][15][16][17][18][19][20][21][22][23]. All these applications require the particles to be superparamagnetic at room temperature.…”

Section: Introductionmentioning

confidence: 99%

The Influence of Synthesis Parameters on Structural and Magnetic Properties of Iron Oxide Nanomaterials

Cursaru

Piticescu

Dragut

et al. 2019

Preprint

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Magnetic iron oxide particles are used for in vitro diagnostics for nearly 40 years. Due to their unique physical, chemical, thermal and mechanical properties, as well as biocompatibility and low toxicity in the human body, iron oxide nanoparticles have been used in many biomedical applications, such as contrast agents for magnetic resonance imaging, carriers for controlled drug delivery and immunoassays, but also in magnetic hyperthermia. Our aim is to investigate the effect of pressure and temperature on the structural, thermal and magnetic properties of iron oxide nanomaterials prepared by hydrothermal synthesis. Iron oxide nanoparticles were synthesized at temperatures of 100-200°C and pressures of 20-1000 bar. It has been found that pressure influences the type of iron oxide crystalline phase. Thus, for lower pressure values (< 100 bar), iron oxide is predominantly formed as hematite, while at pressures > 100 bar, the major crystalline phase is goethite. The complex thermal analysis revealed the polymorphic changes of iron oxides at different temperatures. The existence of specific magnetite and hematite phases in all thermally treated samples are evidenced through the specific Verwey and Morin transitions highlighted by ZFC-FC (Zero Field Cooled-Field Cooled) measurements, whereas their relative content is precisely provided by Mössbauer spectroscopy.

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Radiosensitizing properties of magnetic hyperthermia mediated by superparamagnetic iron oxide nanoparticles (SPIONs) on human cutaneous melanoma cell lines

Cited by 44 publications

References 65 publications

<p>Emerging medical applications based on non-ionizing electromagnetic fields from 0 Hz to 10 THz</p>

<p>Emerging medical applications based on non-ionizing electromagnetic fields from 0 Hz to 10 THz</p>

Impact of Superparamagnetic Iron Oxide Nanoparticles on In Vitro and In Vivo Radiosensitisation of Cancer Cells

The Influence of Synthesis Parameters on Structural and Magnetic Properties of Iron Oxide Nanomaterials

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