Radiosensitivity of blood lymphocytes from basocellular carcinoma patients, as detected by the micronucleus assay

Ochi-Lohmann, Tania; Okazaki, Kazuichi; Madruga, Maria Regina; Pereira, Carlos Alberto de Bragança; Rabello-Gay, Maria Nazareth

doi:10.1016/0027-5107(96)00089-9

Cited by 5 publications

(2 citation statements)

References 28 publications

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“…Chromosome analysis has gradually become a major diagnose procedure in several areas of clinical medicine [38]. Also, the human peripheral blood lymphocyte culture constitutes an excellent system to test the capacity of physical or chemical agents to cause DNA damage and can be used to monitor individuals exposed to them [39]. All the methods employed in the primary evaluation of DNA damage include the comet assay, which presents a potential to detect cytotoxic and genotoxic agents [32,37,[40][41][42].…”

Section: Discussionmentioning

confidence: 99%

Mutagenic and Genotoxic Effects of cis-(Dichloro)tetraammineruthenium(III) Chloride on Human Peripheral Blood Lymphocytes

Ribeiro

Silva

Pereira

et al. 2009

Biol Trace Elem Res

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Chemotherapeutic agents play an important role in cancer treatment mostly due their systemic action on human organism allowing access to liquid tumors and even metastases. Among these drugs, ruthenium compounds have been showing promising results to treat tumors and represent an important development of new antitumor therapy. This study presents the evaluation of cis-(dichloro)tetraammineruthenium(III) chloride, cis-[RuCl(2)(NH(3))(4)]Cl, genotoxic effects using human peripheral blood lymphocytes cultured in vitro. Mitotic index (MI), chromosome aberrations (CA), and DNA damage using the comet assay were analyzed. MI in human peripheral blood lymphocyte cultures treated with 1, 10, 100, and 1,000 microg mL(-1) cis-[RuCl(2)(NH(3))(4)]Cl were 5.9%, 4.6%, 3.9%, and 0%, respectively. Doxorubicin chloridate was used as the positive control. CA derived from 1, 10, and 100 microg mL(-1) concentrations were defined as spontaneous when compared with the negative control, and at the concentration of 1,000 microg mL(-1), the cell cycle was inhibited (IM = 0%). Results obtained for the comet assay using cis-[RuCl(2)(NH(3))(4)]Cl suggest that this compound has no genotoxic activity against cultured human peripheral blood lymphocytes.

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Section: Discussionmentioning

confidence: 99%

Mutagenic and Genotoxic Effects of cis-(Dichloro)tetraammineruthenium(III) Chloride on Human Peripheral Blood Lymphocytes

Ribeiro

Silva

Pereira

et al. 2009

Biol Trace Elem Res

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“…It is currently accepted that DNA damage directly or indirectly caused by mutagenic and carcinogenic agents is primarily responsible for chromosomal abnormalities. Therefore, DNA damage deserves special attention from health centers and the scientific community, and its monitoring is useful for routine testing to prevent different pathologies [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

DNA damage and cellular abnormalities in tuberculosis, lung cancer and chronic obstructive pulmonary disease

Silva

Bresciani

Karnopp

et al. 2015

Multidiscip Respir Med

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BackgroundTuberculosis (TB), Lung Cancer (LC) and Chronic Obstructive Pulmonary Diseases (COPD) affect millions of individuals worldwide. Monitoring of DNA damage in pathological situations has been investigated because it can add a new dimension to clinical expression and may represent a potential target for therapeutic intervention. The aim of this study was to evaluate DNA damage and the frequency of cellular abnormalities in TB, LC and COPD patients by comparing them to healthy subjects.MethodsThe detection of DNA damage by a buccal micronucleus cytome assay was investigated in patients with COPD (n = 28), LC (n = 18) and TB (n = 22) and compared to control individuals (n = 17).ResultsThe COPD group had a higher frequency of apoptotic cells compared to TB and LC group. The TB group showed a higher frequency of DNA damage, defect in cytokinesis, apoptotic and necrotic cells. Patients with LC had low frequency of chromosomal aberrations than TB and COPD patients.ConclusionCOPD patients showed cellular abnormalities that corresponded to cell death by apoptosis and necrosis, while patients with TB presented defects in cytokinesis and dysfunctions in DNA repair that resulted in the formation of micronucleus (MN) besides apoptotic and necrotic cells. Patients with COPD, TB and LC had a low frequency of permanent DNA damage.

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Micronuclei frequency in peripheral blood lymphocytes of cancer patients: A meta-analysis

Iarmarcovai

Ceppi²,

Botta

et al. 2008

Mutation Research/Reviews in Mutation Research

135

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Radiosensitivity of blood lymphocytes from basocellular carcinoma patients, as detected by the micronucleus assay

Cited by 5 publications

References 28 publications

Mutagenic and Genotoxic Effects of cis-(Dichloro)tetraammineruthenium(III) Chloride on Human Peripheral Blood Lymphocytes

Mutagenic and Genotoxic Effects of cis-(Dichloro)tetraammineruthenium(III) Chloride on Human Peripheral Blood Lymphocytes

DNA damage and cellular abnormalities in tuberculosis, lung cancer and chronic obstructive pulmonary disease

Micronuclei frequency in peripheral blood lymphocytes of cancer patients: A meta-analysis

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